RESUMO
The influence of exogenous 8-oxo-2'-deoxyguanosine on the development of Drosophila melanogaster under normal conditions, and under the influence of short-term heat shock was studied. It was shown that 8-oxo-2'-deoxyguanosine was not toxic at concentrations of up to 1⯵M. A tendency to accelerate larval development and fly emergence was observed under the influence of this compound in our experiments. Short-term heat shock causes a 50-80% decrease in the number of larvae that complete development. The addition of exogenous 8-oxo-2'-deoxyguanosine to the food before thermal influence negates this effect and brings the levels of the imago emergence indicators back to the basal level. The obtained results are further evidence of the possible bioregulatory and adaptogen functions of 8-oxo-2'-deoxyguanosine.
Assuntos
8-Hidroxi-2'-Desoxiguanosina/farmacologia , Drosophila melanogaster/efeitos dos fármacos , Resposta ao Choque Térmico/efeitos dos fármacos , Larva/efeitos dos fármacos , Animais , DNA/metabolismo , Drosophila melanogaster/crescimento & desenvolvimento , Drosophila melanogaster/metabolismo , Temperatura Alta/efeitos adversos , Larva/crescimento & desenvolvimento , Larva/metabolismoRESUMO
According to one hypothesis, Parkinson's disease pathogenesis is largely caused by dopamine catabolism that is catalyzed on mitochondrial membranes by monoamine oxidase. Reactive oxygen species are formed as a byproduct of these reactions, which can lead to mitochondrial damage followed by cell degeneration and death. In this study, we investigated the effects of administration of the mitochondrial antioxidant SkQ1 on biochemical, immunohistochemical, and behavioral parameters in a Parkinson-like condition caused by protoxin MPTP injections in C57BL/6 mice. SkQ1 administration increased dopamine quantity and decreased signs of sensory-motor deficiency as well as destruction of dopaminergic neurons in the substantia nigra and ventral tegmental area in mice with the Parkinson-like condition.
