Dynamics of Nanosecond Laser Pulse Propagation and of Associated Instabilities in a Magnetized Underdense Plasma.

The propagation and energy coupling of intense laser beams in plasmas are critical issues in inertial confinement fusion. Applying magnetic fields to such a setup has been shown to enhance fuel confinement and heating. Here we report on experimental measurements demonstrating improved transmission and increased smoothing of a high-power laser beam propagating in a magnetized underdense plasma. We also measure enhanced backscattering, which our kinetic simulations show is due to magnetic confinement of hot electrons, thus leading to reduced target preheating.

A simple score derived from bone marrow immunophenotyping is important for prognostic evaluation in myelodysplastic syndromes.

Immunophenotyping of bone marrow (BM) precursors has been used as an ancillary diagnostic tool in myelodysplastic syndromes (MDS), but there is no general agreement about which variables are the most relevant for prognosis. We developed a parsimonious prognostic model based on BM cell populations well-defined by phenotype. We analyzed 95 consecutive patients with primary MDS diagnosed at our Institution between 2005 and 2012 where BM immunophenotyping had been performed at diagnosis. Median follow-up: 42 months (4-199). Median age: 67 years (33-79). According to IPSS-R, 71 cases were low or intermediate risk. Flow variables significant in the univariate Cox analysis: "%monocytes/TNCs", "% CD16+ monocytes/TNCs", "total alterations in monocytes", "% myeloid CD34+ cells", "number of abnormal expressions in myeloblasts" and "% of B-cell progenitors". In the multivariate model remained independent: "% myeloid CD34+ cells", B-cell progenitors" and "% CD16+ monocytes/TNCs". These variables were categorized by the extreme quartile risk ratio strategy in order to build the score: % myeloid CD34+ cells" (≥ 2.0% = 1 point), B-cell progenitors" (< 0.05% 1 point) and "CD16+ monocytes/TNCs" (≥ 1.0% 1 point). This score could separate patients with a different survival. There was a weak correlation between the score and IPSS-R. Both had independent prognostic values and so, the flow score adds value for the prognostic evaluation in MDS.

Productive characteristics in dairy buffalo (Bubalus bubalis) in the Eastern Amazon / Características produtivas em búfalos leiteiros (Bubalus bubalis) na Amazônia

The objective of this study was to determine factors affecting phenotypic characteristics related to milk production and reproductive efficiency of dairy buffalo. Phenotypic records of 2,459 Murrah, Mediterranean and crossbred buffaloes from the Embrapa Eastern Amazônia herd, from 1969 to 2013 were used. Traits included: total milk production during lactation (TMP), milk production corrected for 305 days (P305); cow weight at calving (CW), fat milk percentage (F), age at first calving (AFC), calving interval (CI), calf birth weight (BW), as well as lactation (LP) and service periods (SP). Most traits were affected by genetic group as well as year and season or interaction between them. Genetic group affected F, CI, SP and CW. Very young and older cows showed poorer production traits. There was interaction between season and year for F, CI and CW. Correlations traits were generally medium to low. The principal component analysis showed that higher fat milk percentage was associated with higher percentage of Murrah breed, longer lactation period and older cows. Phenotypic characteristics in buffaloes reared in the Brazilian Amazon were significantly affected by genetic and management factors. This information can be used to help build sustainable production systems for the region and guide farmer decisions.(AU)

O objetivo deste estudo foi determinar os fatores que afetam as características fenotípicas relacionadas à produção de leite e a eficiência reprodutiva de búfalas leiteiras. Foram utilizados os registros fenotípicos de 2.459 búfalas das raças Murrah, Mediterrâneo e seus cruzamentos do rebanho da Embrapa Amazônia Oriental, de 1969 a 2013. As características incluídas foram: produção total de leite durante a lactação (PTL), produção de leite corrigida para 305 dias (P305), peso da búfala ao parto (PV), percentual de gordura do leite (G), idade ao primeiro parto (IPP), intervalo entre partos (IP), peso do bezerro ao nascimento (PN), assim como os períodos de lactação (PL) e de serviço (PS). A maioria das características foi afetada pelo grupo genético, bem como pelo ano e pela estação ou pela interação entre eles. O grupo genético afetou o G, IP, PS e PV. Búfalas muito jovens e mais velhas mostraram características de produção mais baixas. Houve interação entre estação e o ano para G, IP e PV. As correlações entre as características foram geralmente de médias a baixas. A análise de componentes principais mostrou que a maior porcentagem de gordura no leite foi associada com a maior porcentagem de sangue Murrah, com o maior período de lactação e com búfalas mais velhas. As características fenotípicas das búfalas criadas na Amazônia brasileira foram significativamente afetadas por fatores genéticos e de manejo. Essas informações podem ser usadas para ajudar a construir sistemas de produção sustentáveis para a região e orientar as decisões dos produtores.(AU)

Laser-Plasma Interaction Experiment for Solar Burst Studies.

A new experimental platform based on laser-plasma interaction is proposed to explore the fundamental processes of wave coupling at the origin of interplanetary radio emissions. It is applied to the study of electromagnetic (EM) emission at twice the plasma frequency (2ω_{p}) observed during solar bursts and thought to result from the coalescence of two Langmuir waves (LWs). In the interplanetary medium, the first LW is excited by electron beams, while the second is generated by electrostatic decay of Langmuir waves. In the present experiment, instead of an electron beam, an energetic laser propagating through a plasma excites the primary LW, with characteristics close to those at near-Earth orbit. The EM radiation at 2ω_{p} is observed at different angles. Its intensity, spectral evolution, and polarization confirm the LW-coalescence scenario.

Genetic evaluation of milking buffaloes (Bubalus bubalis): bull ranking / Avaliação genética de búfalas leiteiras (Bubalus bubalis): classificação de touros

The objective of this study was to evaluate genetic aspects related to production and reproductive efficiency of Murrah and Mediterranean buffaloes and their crosses. A ranking of bulls from Embrapa Eastern Amazonia was also composed to guide assisted mating. Birth records of 2,322 Murrah, Mediterranean, and crossbred buffaloes from the Embrapa Eastern Amazon herd, from 1953 to 2013, as well as information on production and reproductive traits were used. Genetic analyzes were performed in the WOMBAT software using the animal model with two-trait analysis. While heritability (h2) for total milk production (TMP) and fat milk percentage (F) were generally high, for reproductive traits h2 tended to be low. Genetic correlations for TMP with the other traits were low and negative, except for TMP with calving interval (CI) and service period (SP) in the Mediterranean breed and with age at first calving (AFC) and SP in crossbred, which were positive and high. Bull 1001 had high predicted transmitting ability (PTA) for TMP, so it should transmit a greater volume of milk to his offspring, although it had a lower PTA for F. There was sufficient variability within the herd to work with genetic management for both production and reproductive efficiency.(AU)

O objetivo deste estudo foi avaliar os aspectos genéticos relacionados à produção e à eficiência reprodutiva de búfalas das raças Murrah, Mediterrâneo e suas cruzas. Uma classificação de touros da Embrapa Amazônia Oriental também foi composta para orientar os cruzamentos assistidos. Foram utilizados 2.322 registros de nascimento de búfalas das raças Murrah, Mediterrâneo e cruzadas do rebanho da Embrapa Amazônia Oriental, de 1953 a 2013, bem como características produtivas e reprodutivas. As análises genéticas foram realizadas pelo software Wombat, utilizando-se o modelo animal com análise de duas características. Enquanto a herdabilidade (h 2 ) para a produção total de leite (PTL) e para a porcentagem de gordura (G) foi alta, para as características reprodutivas a h 2 tendeu a ser baixa. As correlações genéticas da PTL com as demais características foram baixas e negativas, exceto para a PTL com intervalo entre partos (IP) e período de serviço (PS) na raça Mediterrâneo e com idade ao primeiro parto (IPP) e PS nas cruzadas, que foram positivas e altas. O touro 1001 apresentou alta capacidade de transmissão predita (CTP) para a PTL, então deve transmitir um maior volume de leite para seus descendentes, embora com um menor conteúdo transmissível de CTP para G. Portanto, existe variabilidade suficiente dentro do rebanho para trabalhar com o manejo genético para a produção e a eficiência reprodutiva.(AU)

Development of gas jet targets for laser-plasma experiments at near-critical density.

Computational fluid dynamics simulations are performed to design gas nozzles, associated with a 1000 bars backing pressure system, capable of generating supersonic gas jet targets with densities close to the critical density for 1053 nm laser radiation (1021 cm-3). Such targets should be suitable for laser-driven ion acceleration at a high repetition rate. The simulation results are compared to the density profiles measured by interferometry, and characterization of the gas jet dynamics is performed using strioscopy. Proton beams with maximum energies up to 2 MeV have been produced from diatomic hydrogen gas jet targets in a first experiment.

Experimental evidence for the enhanced and reduced stopping regimes for protons propagating through hot plasmas.

Our understanding of the dynamics of ion collisional energy loss in a plasma is still not complete, in part due to the difficulty and lack of high-quality experimental measurements. These measurements are crucial to benchmark existing models. Here, we show that such a measurement is possible using high-flux proton beams accelerated by high intensity short pulse lasers, where there is a high number of particles in a picosecond pulse, which is ideal for measurements in quickly expanding plasmas. By reducing the energy bandwidth of the protons using a passive selector, we have made proton stopping measurements in partially ionized Argon and fully ionized Hydrogen plasmas with electron temperatures of hundreds of eV and densities in the range 1020-1021 cm-3. In the first case, we have observed, consistently with previous reports, enhanced stopping of protons when compared to stopping power in non-ionized gas. In the second case, we have observed for the first time the regime of reduced stopping, which is theoretically predicted in such hot and fully ionized plasma. The versatility of these tunable short-pulse laser based ion sources, where the ion type and energy can be changed at will, could open up the possibility for a variety of ion stopping power measurements in plasmas so long as they are well characterized in terms of temperature and density. In turn, these measurements will allow tests of the validity of existing theoretical models.

Guiding of relativistic electron beams in dense matter by laser-driven magnetostatic fields.

Intense lasers interacting with dense targets accelerate relativistic electron beams, which transport part of the laser energy into the target depth. However, the overall laser-to-target energy coupling efficiency is impaired by the large divergence of the electron beam, intrinsic to the laser-plasma interaction. Here we demonstrate that an efficient guiding of MeV electrons with about 30 MA current in solid matter is obtained by imposing a laser-driven longitudinal magnetostatic field of 600 T. In the magnetized conditions the transported energy density and the peak background electron temperature at the 60-µm-thick target's rear surface rise by about a factor of five, as unfolded from benchmarked simulations. Such an improvement of energy-density flux through dense matter paves the ground for advances in laser-driven intense sources of energetic particles and radiation, driving matter to extreme temperatures, reaching states relevant for planetary or stellar science as yet inaccessible at the laboratory scale and achieving high-gain laser-driven thermonuclear fusion.

Exposure to crack cocaine causes adverse effects on marine mussels Perna perna.

Our study aimed to evaluate crack cocaine effects in different life stages of the marine mussel Perna perna. For this purpose, fertilization rate, embryo-larval development, lysosomal membrane stability and DNA strand breaks were assessed. Effect concentrations in gametes and in larval development were found after 1h (IC50=23.53mg·L-1) and 48h (IC50=16.31mg·L-1), respectively. The highest tested concentration showing no acute toxicity (NOEC) was 10mg·L-1, while the lowest observed effect concentration (LOEC) was 20mg·L-1. NOEC concerning embryo-larval development was 0.625mg·L-1, while the LOEC was 1.25mg·L-1. Cyto-genotoxic effects were evidenced in mussels exposed to crack cocaine concentrations ranging from 5 to 500µg·L-1. Our results report the first data on effects of an illicit drug to marine organisms and should encourage further ecotoxicological studies of these contaminants of emerging concern in coastal ecosystems.

Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon.

Water buffaloes (Bubalus bubalis) are quite well adapted to climatic conditions in the Amazon, and in this biome, they are noted for the considerable amount of meat and milk they produce and how hard they are able to work. Because of a lack of research dedicated to improving the rearing of buffaloes in the Amazon, the objective of this study was to genetically characterize the Murrah and Mediterranean breeds, as well as a mixed-breed population, based on polymorphisms in the diacylglycerol O-acyltransferase 1 gene (DGAT1), and associate the genotypes with milk production. By using the polymerase chain reaction-single-strand conformation polymorphism technique, the alleles A (0.79), B (0.20), and D (0.01) were found in the Murrah breed. In the Mediterranean and mixed-breed buffaloes, we found alleles A (0.69) and (0.77) and B (0.31) and (0.23), respectively. The Murrah breed had the genotypes AA (0.63), AB (0.29), BB (0.05), and AD (0.03), and the Mediterranean and mixed-breed buffaloes had the genotypes AA (0.44) and (0.61), AB (0.50) and (0.31), and BB (0.06) and (0.08), respectively. For the Murrah, Mediterranean, and mixed-breed buffaloes, respectively, the expected heterozygosity values were 0.34, 0.43, and 0.35, the inbreeding coefficients were 0.78, -0.15, and 0.17, and the Hardy-Weinberg probabilities were 0.70, 0.67, and 0.52. The genotypes evaluated did not have an effect on milk production; however, the single nucleotide polymorphisms can be used in studies on genetic variability.

Signatures of the Self-Similar Regime of Strongly Coupled Stimulated Brillouin Scattering for Efficient Short Laser Pulse Amplification.

Plasma-based laser amplification is considered as a possible way to overcome the technological limits of present day laser systems and achieve exawatt laser pulses. Efficient amplification of a picosecond laser pulse by stimulated Brillouin scattering (SBS) of a pump pulse in a plasma requires to reach the self-similar regime of the strongly coupled (SC) SBS. In this Letter, we report on the first observation of the signatures of the transition from linear to self-similar regimes of SC-SBS, so far only predicted by theory and simulations. With a new fully head-on collision geometry, subpicosecond pulses are amplified by a factor of 5 with energy transfers of few tens of mJ. We observe pulse shortening, frequency spectrum broadening, and down-shifting for increasing gain, signatures of SC-SBS amplification entering the self-similar regime. This is also confirmed by the power law dependence of the gain on the amplification length: doubling the interaction length increases the gain by a factor 1.4. Pump backward Raman scattering (BRS) on SC-SBS amplification has been measured for the first time, showing a strong decrease of the BRS amplitude and frequency bandwidth when SBS seed amplification occurs.

Monochromatic short pulse laser produced ion beam using a compact passive magnetic device.

High-intensity laser accelerated protons and ions are emerging sources with complementary characteristics to those of conventional sources, namely high charge, high current, and short bunch duration, and therefore can be useful for dedicated applications. However, these beams exhibit a broadband energy spectrum when, for some experiments, monoenergetic beams are required. We present here an adaptation of conventional chicane devices in a compact form (10 cm × 20 cm) which enables selection of a specific energy interval from the broadband spectrum. This is achieved by employing magnetic fields to bend the trajectory of the laser produced proton beam through two slits in order to select the minimum and maximum beam energy. The device enables a production of a high current, short duration source with a reproducible output spectrum from short pulse laser produced charged particle beams.

Amplification of ultrashort laser pulses by brillouin backscattering in plasmas.

Plasma media, by exciting Raman (electron) or Brillouin (ion) waves, have been used to transfer energy from moderately long, high-energy light pulses to short ones. Using multidimensional kinetic simulations, we define here the optimum window in which a Brillouin scheme can be exploited for amplification and compression of short laser pulses over short distances to very high power. We also show that shaping the plasma allows for increasing the efficiency of the process while minimizing other unwanted plasma processes. Moreover, we show that, contrary to what was traditionally thought (i.e., using Brillouin in gases for nanosecond pulse compression), this scheme is able to amplify pulses of extremely short duration.

Índices de eficiência reprodutiva de búfalos da Amazônia Oriental do Brasil / Indices of reproductive efficiency of buffaloes of the Brazilian Eastern Amazon

Foram avaliados registros de manejo reprodutivo de búfalos das raças Jafarabadi, Murrah, Mediterrâneo, Carabao e Tipo Baio, bem como seus mestiços, no período de 1983 a 2005. Estudaram-se as características reprodutivas: idade à primeira cria (média de 1052,52±120,45 dias), intervalo de partos (média de 399,69±23,78 dias), eficiência reprodutiva (média de 91,09±1,89%), fertilidade real adaptada (média de 29,30±4,40 quilogramas de bezerro parido por ano), produtividade ao primeiro parto adaptada (média de 33,75±6,89 quilogramas) e produtividade acumulada adaptada (média de 22,86±6,55 quilogramas de bezerro parido por ano). Foram verificadas influências do ano de parto e o grau de sangue da fêmea sobre as características estudadas, o que indica que tais efeitos devem ser incluídos na avaliação genética e na seleção dos animais. Os índices produtivos utilizados mostraram-se de grande valia para a seleção, devendo ser mais estudadas suas interações relativas ao desempenho geral do rebanho.

We evaluated records of reproductive management Jaffarabadi buffalo breeds, Murrah, Mediterranean, Carabao and Baio types, and their crosses, in the period from 1983 to 2005. We studied the following reproductive characteristics: Age at first calving (mean 1052.52±120.45 days), calving interval (399.69±23.78 mean days), Reproductive Efficiency (average 91.09±1, 89%), Real Adapted Fertility (average 29.30±4.40kg calf calved each year), the First Labor Productivity Adapted (mean 33.75±6.89kg) and Accumulated Productivity Adapted (mean 22.86±6.55kg calf calved each year). Influences were observed in the year of delivery and degree of blood on the female traits, suggesting that such effects should be included in genetic evaluation and selection of animals. The production indices used were of great value for the selection, and should be well studied for their interactions with the overall performance of the herd.

Genetic characterization of Latin-American Creole cattle using microsatellite markers.

Genetic diversity in and relationships among 26 Creole cattle breeds from 10 American countries were assessed using 19 microsatellites. Heterozygosities, F-statistics estimates, genetic distances, multivariate analyses and assignment tests were performed. The levels of within-breed diversity detected in Creole cattle were considerable and higher than those previously reported for European breeds, but similar to those found in other Latin American breeds. Differences among breeds accounted for 8.4% of the total genetic variability. Most breeds clustered separately when the number of pre-defined populations was 21 (the most probable K value), with the exception of some closely related breeds that shared the same cluster and others that were admixed. Despite the high genetic diversity detected, significant inbreeding was also observed within some breeds, and heterozygote excess was detected in others. These results indicate that Creoles represent important reservoirs of cattle genetic diversity and that appropriate conservation measures should be implemented for these native breeds in order to minimize inbreeding and uncontrolled crossbreeding.

Variabilidade genética de búfalos em rebanho-núcleo com base na análise de pedigree / Genetic variability in water buffalo from nucleous herd by pedigree analysis

Parâmetros baseados na probabilidade de origem do gene foram usados para descrever a variabilidade em uma população de búfalos da Embrapa Amazônia Oriental. A magnitude dos resultados foi de média a baixa (por volta de 20 animais), sugerindo que poucos fundadores contribuiriam para a formação da população. Dentre os 20 ancestrais que mais aportam genes aos machos - representando ao todo 71 por cento dos alelos -, 39 por cento, 26 por cento e 5 por cento, respectivamente, são as contribuições marginais das raças Murrah e Mediterrâneo e seus mestiços. Para as fêmeas, em que os 20 ancestrais aportam 67,5 por cento dos genes, 42 por cento e 26 por cento, respectivamente, são as contribuições marginais das raças Murrah e Mediterrâneo.

Parameters based on the probability of gene origin were used to describe genetic variability in a buffalo population from the Embrapa Amazônia Oriental, Belém, Pará, Brazil. The parameters generated medium to low values (around 20 animals) and suggested low founder representativeness. From the 20 ancestors that gave more genes to males (with 71 percent of alleles), genetic contributions were 39 percent, 26 percent, and 5 percent, respectively, for Murrah, Mediterraneo, and crossbreds. For females, these values were 42 percent and 26 percent for Murrah and Mediterraneo breeds.

Estimação de parâmetros genéticos para características produtivas em búfalos na Amazônia Oriental / Estimation of genetic parameters of dairy buffaloes productive characteristics Eastern Amazon

Dados de 1.182 registros de produção de fêmeas bubalinas da raça Murrah e seus mestiços, parindo no período de 1967 a 2005, foram utilizados para estimação de parâmetros genéticos utilizando-se o método de máxima verossimilhança restrita. O modelo animal utilizado para estimação de componentes de variância incluiu os efeitos fixos de rebanho, ano e época de parto, ordem de parto e duração da lactação e os efeitos aleatórios do animal, e ambiente permanente e temporário. As estimativas de herdabilidade foram 0,25, 0,18, 0,08 e 0,09, para produção de leite, produção de gordura, duração da lactação e produção de leite por dia de intervalo de parto, respectivamente. As estimativas de repetibilidade foram 0,33, 0,29 e 0,10 para produção de leite, produção de gordura e duração da lactação, respectivamente. As correlações genéticas entre produções de leite e gordura, produção de leite com duração da lactação, produção de leite com produção de leite por dia de intervalo de partos, produção da gordura com duração da lactação, produção de gordura com produção de leite por dia de intervalo de partos e duração da lactação com produção de leite por dia de intervalo de partos foram 0,93; 0,76; 0,99; 0,89; 0,87 e -0,27, respectivamente. Os resultados demonstram que ganhos genéticos podem ser obtidos pela seleção das produções de leite e gordura.

Data from 1,182 records of Murrah females buffaloes and their crossbreds, calving from 1967 to 2005, were used to estimate genetic parameters by maximum restricted likelihood method. An animal model used to estimate variance components, included the fixed effects of herds, year and season of calving, order of parity, and length of lactation as covariables; and random effects of animal, permanent, and temporary environment. The estimate of heritability to milk yield, fat yield, length of lactation, and milk yield per day of calving interval were 0.25, 0.18, 0.08, and 0.09 respectively. Estimate of repeatability for milk yield, fat yield, and length of lactation were 0.33, 0.29, and 0.10, respectively. The genetic correlations between milk yield and fat yield, milk yield and length of lactation, milk yield and milk yield per day of calving interval, fat yield and length of lactation, fat yield and milk yield per day of calving interval, and length of lactation and milk yield per day of calving interval were 0.93, 0.76, 0.99, 0.89, 0.87, and -0.27, respectively. These results showed that genetic gains may be obtained by selecting to milk and fat yields.

Experimental evidence of short light pulse amplification using strong-coupling stimulated brillouin scattering in the pump depletion regime.

The energy transfer from a long (3.5 ps) pump pulse to a short (400 fs) seed pulse due to stimulated Brillouin backscattering in the strong-coupling regime is investigated. The two pulses, both at the same wavelength of 1.057 microm are quasicounterpropagating in a preformed underdense plasma. Relative amplification factors for the seed pulse of up to 32 are obtained. The maximum obtained amplified energy is 60 mJ. Simulations are in agreement with the experimental results and suggest paths for further improvement of the amplification scheme.

Effects of gustatory stimulants of salivary secretion on salivary pH and flow: a randomized controlled trial.

OBJECTIVES: To compare salivary pH changes and stimulation efficacy of two different gustatory stimulants of salivary secretion (GSSS). SETTING: Portuguese Dental Faculty Clinic. DESIGN: Double blind randomized controlled trial. SUBJECTS: One hundred and twenty volunteers were randomized to two intervention groups. Sample sized was calculated using an alpha error of 0.05 and a beta of 0.20. MATERIALS AND METHODS: Participants were randomly assigned to receive a new gustatory stimulant of secretory secretion containing a weaker malic acid, fluoride and xylitol or a traditionally citric acid-based one. Saliva collection was obtained by established methods at different times. The salivary pH of the samples was determined with a pH meter and a microelectrode. MAIN OUTCOME MEASURES: Salivary pH variations and counts of subjects with pH below 5.5 for over 1 min and stimulated salivary flow were the main outcome measures. RESULTS: Both GSSS significantly stimulated salivary output without significant differences between the two groups. The new gustatory stimulant of salivary secretion presented a risk reduction of 80 +/- 10.6% (95% CI) when compared with the traditional one. CONCLUSIONS: Gustatory stimulants of salivary secretion with fluoride, xylitol and lower acid content maintain similar salivary stimulation capacity while reducing significantly the dental erosion predictive potential.

Intense gamma-ray source in the giant-dipole-resonance range driven by 10-TW laser pulses.

A gamma-ray source with an intense component around the giant dipole resonance for photonuclear absorption has been obtained via bremsstrahlung of electron bunches driven by a 10-TW tabletop laser. 3D particle-in-cell simulation proves the achievement of a nonlinear regime leading to efficient acceleration of several sequential electron bunches per each laser pulse. The rate of the gamma-ray yield in the giant dipole resonance region (8