RESUMO
Many Neglected Tropical Diseases (NTDs) have recently been subject of increased focus, particularly with relation to high-throughput screening (HTS) initiatives. These vital endeavours largely rely of two approaches, in vitro target-directed screening using biochemical assays or cell-based screening which takes no account of the target or targets being hit. Despite their successes both of these approaches have limitations; for example, the production of soluble protein and a lack of cellular context or the problems and expense of parasite cell culture. In addition, both can be challenging to miniaturize for ultra (u)HTS and expensive to utilize. Yeast-based systems offer a cost-effective approach to study and screen protein targets in a direct-directed manner within a eukaryotic cellular context. In this review, we examine the utility and limitations of yeast cell-based, target-directed screening. In particular we focus on the currently under-explored possibility of using such formats in uHTS screening campaigns for NTDs.
Assuntos
Bioensaio/estatística & dados numéricos , Avaliação Pré-Clínica de Medicamentos , Ensaios de Triagem em Larga Escala/estatística & dados numéricos , Saccharomyces cerevisiae/genética , Bioensaio/economia , Doenças Transmissíveis/tratamento farmacológico , Sistemas de Liberação de Medicamentos , Descoberta de Drogas , Drogas em Investigação/farmacologia , Expressão Gênica , Engenharia Genética , Ensaios de Triagem em Larga Escala/economia , Humanos , Doenças Negligenciadas/tratamento farmacológico , Proteínas de Protozoários/genética , Proteínas de Protozoários/metabolismo , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/metabolismo , Medicina TropicalRESUMO
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