RESUMO
No disponible
Assuntos
Pessoa de Meia-Idade , Feminino , Humanos , Articulação do Quadril , Fatores de Tempo , Tuberculose Osteoarticular , DorRESUMO
With the hypothesis of a possible helpful effect of the liver on islets transplanted into it, we have performed experiments that suggest some effect of hepatic cells for islet tolerance. We have studied 6 groups of Wistar rats made diabetic with streptozotocin and transplanted in sham conditions and with a mixture of islets and hepatic cells (allo-co-transplantation) in several conditions, all of them via the portal vein, and observed them over 30 days. Groups were as follows: Group A had a sham transplantation with saline. Group B was transplanted with hepatic cells alone. Group C was transplanted with islets alone without hepatic cells. Group D was co-transplanted with cultured islets and fresh hepatic cells (ratio 1:100). Group E was as group D with a ratio of 1:200. Group F also had co-transplantation, but after co-culture of islets and hepatic cells for 24 hours. Results show reversion of diabetes in group D for 4-5 days, and thereafter, a fall of blood glucose during the period observed. The effect was less marked in group F after co-culture of islets and hepatic cells. Paradoxically, when the ratio of islets and hepatic cell were 1:200, the results were not so good. These results suggest that hepatic cells have some helpful effect on islets when co-transplanted in the liver via the portal vein. More studies are needed to clarify if this effect can be related to some hepatic cell subpopulation; also if the effect is a membrane one, cell-to-cell contact, or through some secreted product.
Assuntos
Hepatócitos/fisiologia , Imunossupressores/farmacologia , Transplante das Ilhotas Pancreáticas/fisiologia , Animais , Glicemia/metabolismo , Transplante de Células , Técnicas de Cocultura , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/metabolismo , Masculino , Ratos , Ratos WistarRESUMO
The incidence and clinical characteristics of mycobacteriosis in patients attending the nephrology department in a hospital in Madrid, Spain, during a 30-month period were analysed retrospectively. Twenty-two new cases of tuberculosis were detected among 525 patients studied. No cases of clinically significant atypical mycobacteriosis were found. The estimated overall annual incidence of tuberculosis in these patients was 259 cases per 100,000 population, which is much higher than the national annual incidence in Spain of 35 per 100,000 population. Most cases were asymptomatic when diagnosed and extrapulmonary involvement was the rule (86% of all patients). In areas with a high prevalence of tuberculosis, renal patients in high-risk groups (renal transplant recipients, haemodialysis and CAPD patients) should be examined periodically to exclude silent infection.
Assuntos
Falência Renal Crônica/complicações , Tuberculose/epidemiologia , Humanos , Incidência , Transplante de Rim , Diálise Peritoneal Ambulatorial Contínua , Diálise Renal , Estudos Retrospectivos , Fatores de Risco , Espanha/epidemiologia , Tuberculose/complicaçõesRESUMO
A total of 67 cases of tuberculosis was diagnosed in the first 100 cases of AIDS, diagnosed according to the former CDC criteria, at a hospital in Madrid, Spain. This is the highest known prevalence of tuberculosis in AIDS patients both within and outside Spain. The clinical manifestations of tuberculosis were very variable and atypical. The rate of isolation of Mycobacterium tuberculosis from blood was particularly high: of 25 patients in whom blood cultures were performed, 16 were positive. In a third of the patients with proven mycobacteremia, blood was the first or the only positive specimen. In general, therapy resulted in rapid clinical improvement, but in some cases mycobacteria were isolated from clinical or necroscopy specimens months after what was considered adequate therapy.
