RESUMO
Twenty-one exopolysaccharide-producing strains were isolated from the 5-20 microns fraction of a vertisol in the south-east of the island of Martinique in the French West Indies. Although these strains were phenotypically identified as Burkholderia cepacia or as Burkholderia glathei using BIOLOG microplates, they did not cluster genotypically by amplified rDNA restriction analysis (ARDRA) with any described Burkholderia species. A phylogenetic analysis revealed that the rrs (16S rDNA) sequences of three representative strains clustered in a single branch within the genus Burkholderia and distantly from all of the previously described species of Burkholderia for which rrs sequences were available. DNA-DNA hybridization data as well as phenotypic analyses indicated that the 21 isolates represented a single and new species for which the name Burkholderia caribensis sp. nov. is proposed (type strain MWAP64T = LMG 18531T).
Assuntos
Burkholderia/classificação , Burkholderia/fisiologia , Polissacarídeos Bacterianos/biossíntese , Microbiologia do Solo , Burkholderia/isolamento & purificação , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Genes de RNAr , Martinica , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Fenótipo , Filogenia , Reação em Cadeia da Polimerase/métodos , RNA Ribossômico 16S/genética , Mapeamento por Restrição , Análise de Sequência de DNARESUMO
The marginal opening of a fixed splint on movable and firm abutments was investigated. Regular-body poly(vinyl siloxane) was used as a luting material to cement a three-unit, cast complete crown splint on three metal dies in an acrylic resin model. The middle abutment was set first in acrylic resin and subsequently in a displaceable material. The cement replica was recovered, measured, and compared. The mean cement thickness for the mobile displaceable abutment was significantly greater (P less than 0.001) than that of nonmobile teeth.
