GPR Mapping of Cavities in Complex Scenarios with a Combined Time-Depth Conversion.

The paper deals with a combined time-depth conversion strategy able to improve the reconstruction of voids embedded in an opaque medium, such as cavities, caves, empty hypogeal rooms, and similar targets. The combined time-depth conversion accounts for the propagation velocity of the electromagnetic waves both in free space and in the embedding medium, and it allows better imaging and interpretation of the underground scenario. To assess the strategy's effectiveness, ground penetrating radar (GPR) data referred to as an experimental test in controlled conditions are accounted for and processed by two different approaches to achieve focused images of the scenario under test. The first approach is based on a classical migration algorithm, while the second one faces the imaging as a linear inverse scattering approach. The results corroborate that the combined time-depth conversion improves the imaging in both cases.

Multi-Sensors Geophysical Monitoring for Reinforced Concrete Engineering Structures: A Laboratory Test.

Non-destructive tests are strongly required in engineering applications for monitoring civil structures. The use of compared and integrated innovative approaches based on geophysical methodologies represents an effective tool for the characterization and monitoring of reinforced concrete (RC) structures. Therefore, the main aim of the work was to improve the knowledge on the potentiality and limitations of the Ground Penetrating Radar (GPR) and the Electrical Resistivity Tomography (ERT) with electrodes disposed both on the surface and in the boreholes. The work approach was adopted on an analog model of a reinforced concrete frame built ad hoc at the Hydrogeosite Laboratory (CNR-IMAA), where simulated experiments on full-size physical models are defined. Results show the ability of an accurate use of GPR to reconstruct the rebar dispositions and detect in detail possible constructive defects, both highlighting the lack of reinforcements into the nodes and providing useful information about the safety assessment of the realized structure. The results of the ERT method defined the necessity to develop ad-hoc electrical resistivity methods to support the characterization and monitoring of buried foundation structures for civil engineering applications. Finally, the paper introduces a new approach based on the use of cross-hole ERTs (CHERTs) for the engineering structure monitoring, able to reduce the uncertainties usually affecting the indirect results.

Isolation and characterization of environmental bacteria capable of extracellular biosorption of mercury.

Accumulation of toxic metals in the environment represents a public health and wildlife concern. Bacteria resistant to toxic metals constitute an attractive biomass for the development of systems to decontaminate soils, sediments, or waters. In particular, biosorption of metals within the bacterial cell wall or secreted extracellular polymeric substances (EPS) is an emerging process for the bioremediation of contaminated water. Here the isolation of bacteria from soil, effluents, and river sediments contaminated with toxic metals permitted the selection of seven bacterial isolates tolerant to mercury and associated with a mucoid phenotype indicative of the production of EPS. Inductively coupled plasma-optical emission spectroscopy and transmission electron microscopy in conjunction with X-ray energy dispersive spectrometry revealed that bacteria incubated in the presence of HgCl2 sequestered mercury extracellularly as spherical or amorphous deposits. Killed bacterial biomass incubated in the presence of HgCl2 also generated spherical extracellular mercury deposits, with a sequestration capacity (40 to 120 mg mercury per g [dry weight] of biomass) superior to that of live bacteria (1 to 2 mg mercury per g [dry weight] of biomass). The seven strains were shown to produce EPS, which were characterized by Fourier transform-infrared (FT-IR) spectroscopy and chemical analysis of neutral-carbohydrate, uronic acid, and protein contents. The results highlight the high potential of Hg-tolerant bacteria for applications in the bioremediation of mercury through biosorption onto the biomass surface or secreted EPS.