RESUMO
A label-free-based fiber optic biosensor based on etched tilted Bragg fiber grating (TFBG) is proposed and practically demonstrated. Conventional phase mask technic has been utilized to inscribe tilted fiber Bragg grating with a tilt angle of 10°, while the etching has been accomplished with hydrofluoric acid. A composite of polyethylenimine (PEI)/poly(acrylic acid) (PAA) has been thermally deposited on the etched TFBG, followed by immobilization of probe DNA (pDNA) on this deposited layer. The hybridization of pDNA with the complementary DNA (cDNA) has been monitored using wavelength-dependent interrogation. The reproducibility of the probes has been demonstrated by fabricating three identical probes and their response has been investigated for cDNA concentration ranging from 0 µM to 3 µM. The maximum sensitivity has been found to be 320 pm/µM, with the detection limit being 0.65 µM. Furthermore, the response of the probes towards non-cDNA has also been investigated in order to establish its specificity.
