Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 98
Filtrar
1.
J Dent Res ; 94(3): 446-54, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25576470

RESUMO

Thermal and mechanical hypersensitivity in the injured region is a common complication. Although it is well known clinically that thermal and mechanical sensitivity of the oral mucosa is different from that of the skin, the mechanisms underlying injured pain of the oral mucosa remain poorly understood. The transient receptor potential (TRP) vanilloid 1 (TRPV1) and TRP ankyrin 1 (TRPA1) in primary afferent neurons are known to contribute to pathological pain. Therefore, we investigated whether TRPV1 and/or TRPA1 contribute to thermal and mechanical hypersensitivity following oral mucosa or whisker pad skin incision. Strong heat and mechanical and cold hypersensitivity was caused in the buccal mucosa and whisker pad skin following incisions. On day 3 after the incisions, the number of TRPV1-immunoreactive (IR) and TRPA1-IR trigeminal ganglion (TG) neurons innervating the buccal mucosa and whisker pad skin was significantly increased, and the number of TRPV1/TRPA1-IR TG neurons innervating whisker pad skin, but not the buccal mucosa, was significantly increased. Administration of the TRPV1 antagonist, SB366791, to the incised site produced a significant suppression of heat hyperalgesia in both the buccal mucosa and whisker pad skin, as well as mechanical allodynia in the whisker pad skin. Administration of the TRPA1 antagonist, HC-030031, to the incised site suppressed mechanical allodynia and cold hyperalgesia in both the buccal mucosa and whisker pad skin, as well as heat hyperalgesia in the whisker pad skin. These findings indicate that altered expressions of TRPV1 and TRPA1 in TG neurons are involved in thermal and mechanical hypersensitivity following the buccal mucosa and whisker pad skin incision. Moreover, diverse changes in the number of TRPV1 and TRPA1 coexpressed TG neurons in whisker pad skin-incised rats may contribute to the intracellular interactions of TRPV1 and TRPA1 associated with whisker pad skin incision, whereas TRPV1 and TRPA1 expression in individual TG neurons is involved in buccal mucosa-incised pain.


Assuntos
Dor Facial/fisiopatologia , Mucosa Bucal/lesões , Dor/fisiopatologia , Canais de Cátion TRPC/fisiologia , Canais de Cátion TRPV/fisiologia , Acetanilidas/farmacologia , Anilidas/farmacologia , Animais , Cinamatos/farmacologia , Temperatura Baixa , Eletromiografia/métodos , Temperatura Alta , Hiperalgesia/fisiopatologia , Masculino , Mucosa Bucal/inervação , Neurônios/citologia , Neurônios/fisiologia , Purinas/farmacologia , Ratos , Ratos Sprague-Dawley , Canal de Cátion TRPA1 , Canais de Cátion TRPC/análise , Canais de Cátion TRPC/antagonistas & inibidores , Canais de Cátion TRPV/análise , Canais de Cátion TRPV/antagonistas & inibidores , Gânglio Trigeminal/fisiopatologia , Vibrissas/lesões , Vibrissas/inervação
2.
Br J Neurosurg ; 27(2): 259-61, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23163298

RESUMO

Radiation-induced gliomas are uncommon and therapeutic options are limited due to prior exposure to radiotherapy. Meanwhile, the chemotherapeutic response of anaplastic ependymoma, another rare entity in adults, is often disappointing. We report on the first recorded case of radiation-induced anaplastic ependymoma, in which an excellent clinical response to temozolomide was demonstrated.


Assuntos
Antineoplásicos Alquilantes/uso terapêutico , Dacarbazina/análogos & derivados , Ependimoma/tratamento farmacológico , Neoplasias Induzidas por Radiação/tratamento farmacológico , Segunda Neoplasia Primária/tratamento farmacológico , Adulto , Astrocitoma/radioterapia , Astrocitoma/cirurgia , Neoplasias Cerebelares/radioterapia , Neoplasias Cerebelares/cirurgia , Dacarbazina/uso terapêutico , Feminino , Humanos , Temozolomida
4.
Minim Invasive Neurosurg ; 48(3): 132-5, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16015488

RESUMO

Sessile hypothalamic hamartoma (HH) often causes intractable epilepsy, which is difficult to control even by microsurgical resection and gamma knife surgery (GKS), especially when the hamartoma is intrahypothalamic, large, or irregularly shaped. We successfully applied radiofrequency ablation (RFA) to reduce its epileptogenicity and to disconnect seizure propagation. The patient was a 26-year-old man who presented with refractory epilepsy and severe mental retardation from age 6 months. He had undergone three surgeries yielding partial resection and conventional irradiation treatments. The residual HH was thin and shaped like a bent plate, attached widely to the floor of the third ventricle. He underwent open RFA via the transcallosal sub-choroidal approach under strict image guidance, which resulted in immediate and remarkable seizure remission without complications. This suggests that open RFA is a minimally invasive technique for an irregularly shaped HH that is difficult to treat by other modalities.


Assuntos
Eletrocoagulação/métodos , Epilepsias Parciais/etiologia , Epilepsias Parciais/cirurgia , Hamartoma/cirurgia , Doenças Hipotalâmicas/cirurgia , Radiocirurgia/métodos , Adulto , Epilepsias Parciais/patologia , Hamartoma/complicações , Hamartoma/patologia , Humanos , Doenças Hipotalâmicas/complicações , Doenças Hipotalâmicas/patologia , Masculino
5.
Clin Neuropathol ; 24(2): 64-8, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-15803805

RESUMO

We report here the case of a 29-year-old woman with cerebellar glioblastma. In the present case, tumor lesions were observed in each cerebellar hemisphere. The left-side lesion was diagnosed as glioblastoma, and the right-side lesion as malignant astrocytoma by histopathology. Immunohistochemistry revealed that the tumor cells of the left-side lesion was positive for p53, whereas epidermal growth factor receptors (EGFR) were negative in tumor cells from both sides. Genetic alterations were investigated using a genome DNA microarray (GenoSensor Array 300), which has led us to define this tumor as a secondary glioblastoma. The clinical presentation and genetic findings of this relatively rare entity are discussed.


Assuntos
Neoplasias Cerebelares/genética , Glioblastoma/genética , Segunda Neoplasia Primária/genética , Adulto , Neoplasias Cerebelares/diagnóstico , Neoplasias Cerebelares/terapia , Feminino , Glioblastoma/diagnóstico , Glioblastoma/terapia , Humanos , Segunda Neoplasia Primária/diagnóstico , Segunda Neoplasia Primária/terapia , Análise de Sequência com Séries de Oligonucleotídeos
6.
Acta Neurochir (Wien) ; 147(5): 543-50; discussion 550, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15812596

RESUMO

BACKGROUND: Image-guided and temperature-controlled radiofrequency thermal ablation techniques were applied to reduce tumor volume and relieve the symptoms caused by extracranial extension of recurrent meningioma. METHOD: We treated two patients with recurrent meningioma, an 81-year-old woman presenting with bulging of the temple and a 68-year-old woman presenting with visual disturbance, facial disfigurement, and sensory disturbance. Neuroimaging in both patients, revealed a large tumor extending extracranially and involving the infratemporal fossa. To avoid injury to important anatomical structures either compressed or entrapped by the tumor, the spatial relation between the planned ablation volume and these structures was confirmed by 3-D reconstruction of the ablation target. During the ablation procedure, local temperatures over the tissue being cauterized were continuously monitored to limit the ablation area to that within the planned volume adjusting RF power. FINDING: Radiofrequency ablation produced tumor necrosis as planned without adverse effects and resulted in swift relief of symptoms and signs with shrinkage of the tumor. CONCLUSION: This technique may be an effective alternative for recurrent meningiomas extending extracranially and for which radical surgical procedures are not indicated.


Assuntos
Ablação por Cateter/métodos , Neoplasias Meníngeas/cirurgia , Meningioma/cirurgia , Recidiva Local de Neoplasia/cirurgia , Neoplasias da Base do Crânio/cirurgia , Idoso , Idoso de 80 Anos ou mais , Exoftalmia/etiologia , Exoftalmia/patologia , Exoftalmia/cirurgia , Feminino , Humanos , Imageamento por Ressonância Magnética , Neoplasias Meníngeas/patologia , Meningioma/patologia , Recidiva Local de Neoplasia/patologia , Neoplasias Orbitárias/secundário , Neoplasias Orbitárias/cirurgia , Neoplasias dos Seios Paranasais/secundário , Neoplasias dos Seios Paranasais/cirurgia , Crânio/patologia , Crânio/cirurgia , Neoplasias da Base do Crânio/patologia , Cirurgia Assistida por Computador/métodos , Resultado do Tratamento
7.
J Clin Pathol ; 58(2): 166-71, 2005 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15677537

RESUMO

BACKGROUND/AIMS: In patients with gliomatosis cerebri (GC), glial fibrillary acidic protein (GFAP) positive cells invade the entire brain, particularly the white matter. Because the nosological definition and histogenesis of GC remain controversial, the morphology and immunohistochemical staining patterns of neoplastic GC cells were compared with those of other gliomas. METHODS: An immunohistochemical analysis of neoplastic cells from four patients with GC and 20 with astrocytic tumours using antibodies against Ki-67, GFAP, and L1, the last of which is a neural cell adhesion molecule putatively related to glioma invasion. RESULTS: GC tumour cells can be divided into two types, those mainly composed of strongly GFAP and L1 positive gemistocytic cells, the other composed of small, GFAP and L1 negative spindle shaped cells. The two types did not differ with respect to Ki-67 positivity. Cells from patients with other gliomas were positive for GFAP but concurrent L1 expression was negative or weakly positive. CONCLUSION: The strong expression of L1 in patients with GC and its poor expression in the 20 patients with other types of glioma, including those with GFAP positive gemistocytic astrocytomas, suggest that L1 expression may play a role in the histogenesis of GC.


Assuntos
Neoplasias Encefálicas/patologia , Neoplasias Neuroepiteliomatosas/patologia , Molécula L1 de Adesão de Célula Nervosa/análise , Adulto , Idoso , Anticorpos Antineoplásicos/análise , Astrocitoma/química , Astrocitoma/patologia , Química Encefálica , Divisão Celular/fisiologia , Feminino , Proteína Glial Fibrilar Ácida/análise , Glioblastoma/química , Glioblastoma/patologia , Humanos , Imuno-Histoquímica/métodos , Antígeno Ki-67/imunologia , Imageamento por Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Neoplasias Neuroepiteliomatosas/química
8.
J Neurol Neurosurg Psychiatry ; 75(3): 466-71, 2004 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-14966166

RESUMO

OBJECTIVE: Application of spatially filtered magnetoencephalography (MEG) to investigate changes in the mechanism of cerebral motor control in patients with tumours around the central sulcus. METHODS: MEG records were made during a repetitive hand grasping task in six patients with gliomas around the central sulcus and in four control subjects. Power decreases in the alpha (8-13 Hz), beta (13-30 Hz), and low gamma bands (30-50 Hz) during the motor tasks (event related desynchronisation, ERD) were analysed statistically with synthetic aperture magnetometry. The tomography of ERD was superimposed on the individual's magnetic resonance image. RESULTS: beta ERD was consistently localised to the contralateral primary sensorimotor cortex (MI/SI) in control subjects, whereas the alpha and low gamma ERD showed considerable intersubject variability. beta ERD in patients during non-affected side hand movement was also localised to the contralateral MI/SI, but exclusively to the ipsilateral hemisphere during affected side hand movement. CONCLUSIONS: The altered pattern of ERD in the patient group during affected side hand movement suggests recruitment of diverse motor areas, especially the ipsilateral MI/SI, which may be required for the effective movement of the affected hand.


Assuntos
Neoplasias Encefálicas/complicações , Glioma/complicações , Transtornos das Habilidades Motoras/etiologia , Adulto , Neoplasias Encefálicas/patologia , Potenciais Evocados , Feminino , Lateralidade Funcional , Glioma/patologia , Mãos/fisiologia , Humanos , Magnetoencefalografia , Masculino , Pessoa de Meia-Idade , Transtornos das Habilidades Motoras/fisiopatologia , Reprodutibilidade dos Testes , Análise e Desempenho de Tarefas
9.
Acta Neurochir (Wien) ; 145(8): 697-700; discussion 700-1, 2003 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-14520551

RESUMO

BACKGROUND: Radiosurgery has been advocated as a primary treatment for hypothalamic hamartoma (HH), but it has a risk of damaging the surrounding structures and does not have an immediate effect for refractory epilepsy, endocrinological and mental disorders. METHOD: We report on a 13-year-old boy with a large and sessile HH who presented with intractable seizures, precocious puberty and aggressiveness. Stereotactic radiofrequency ablation (SRA) combined with an image fusion technique was performed to make a maximum ablative lesion within the HH via multiple trajectories. FINDINGS: After surgery, we observed rapid cessation of the gelastic seizures and aggressiveness. The ophthalmological function did not get worse, and the hypothalamopituitary function improved. INTERPRETATION: SRA in combination with an image fusion technique is a viable alternative treatment for HH, because it provides precise preoperative simulation and immediate improvement of symptoms can be obtained.


Assuntos
Ablação por Cateter/métodos , Hamartoma/complicações , Hamartoma/cirurgia , Doenças Hipotalâmicas/complicações , Doenças Hipotalâmicas/cirurgia , Radiocirurgia/métodos , Convulsões/etiologia , Convulsões/cirurgia , Adolescente , Circulação Cerebrovascular/fisiologia , Hamartoma/fisiopatologia , Humanos , Doenças Hipotalâmicas/fisiopatologia , Imageamento por Ressonância Magnética , Masculino , Convulsões/fisiopatologia , Tomografia Computadorizada por Raios X
10.
Gene Ther ; 9(24): 1653-8, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12457278

RESUMO

A silicone formulation of ganciclovir (GCV-pellet) was developed to enhance the cytotoxic effects of herpes simplex virus thymidine kinase suicide gene therapy. The effectiveness of this drug delivery system was assessed in a rat 9L gliosarcoma model. The GCV-pellets (1 mm in length and in diameter) used in this experiment contained a total amount of 0.15 mg of GCV. In vitro experiments demonstrated that GCV was gradually released over a period of 7 days. Five days after stereotactic tumor inoculation into the right caudate nucleus, a herpes simplex virus type 1 (HSV-1) vector expressing herpes simplex virus thymidine kinase (HSV-tk) (T1, 2x10(6) pfu) was administered at the same location. The survival rate of the group treated with the GCV-pellet was compared with that of the T1 group injected intraperitoneally (IP) with GCV (30 mg/kg/day for 7 days). The GCV-pellet-treated group had a significantly prolonged survival (a median of more than 80 days) compared with the GCV IP group (a median of 65 days) and with control groups (P<0.05). The control groups (untreated or receiving only the virus vector) had a survival of 35-38 days. The survival rate of the GCV-pellet group over 80 days was 75%, and all the rats that survived more than 80 days and did not show tumors upon histological examination of the brain were deemed cured. No toxic effects or immunological reactions were observed histologically around the pellet in brain sections from the rats treated with the GCV-pellet. After GCV-pellet inoculation into the tumor, drug concentrations were kept at 1-10 microg/g tissue for 3-4 days. When the same dose of GCV (0.15 mg) in aqueous solution was injected into the tumor, GCV concentrations reached a peak of 0.5 mg/g tissue after 30 min and decreased below measurable level within 12 h. After IP injections of 3 mg GCV, GCV concentrations in the tumor reached a peak of 5.7 microg/g tissue after 30 min and also decreased below measurable level within 12 h. This sustained release of a low and effective GCV dose with the silicone formulation significantly prolonged survival in combinations with HSV-tk expression if compared to IP administration of GCV. Histological examination suggests that the treatment appears to be safe.


Assuntos
Antivirais/administração & dosagem , Neoplasias Encefálicas/terapia , Ganciclovir/administração & dosagem , Terapia Genética/métodos , Gliossarcoma/terapia , Animais , Antivirais/análise , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/mortalidade , Efeito Espectador , Núcleo Caudado , Preparações de Ação Retardada , Esquema de Medicação , Feminino , Ganciclovir/análise , Vetores Genéticos/administração & dosagem , Gliossarcoma/metabolismo , Gliossarcoma/mortalidade , Herpesvirus Humano 1/enzimologia , Herpesvirus Humano 1/genética , Injeções Intralesionais , Injeções Intraperitoneais , Modelos Animais , Ratos , Ratos Endogâmicos F344 , Silicones , Taxa de Sobrevida , Timidina Quinase/genética
11.
J Neurooncol ; 53(1): 21-6, 2001 May.
Artigo em Inglês | MEDLINE | ID: mdl-11678426

RESUMO

A putative tumor suppressor, the PTEN gene at chromosome 10q23. was identified and found to be mutated in many different human tumors. PTEN was recently found to be also involved in focal cell adhesion and cell migration. To identify the role of PTEN gene in malignant gliomas. we used PCR-SSCP and direct sequencing methods to examine 44 malignant gliomas comprising 29 cases without and 15 cases with meningeal gliomatosis. In malignant gliomas without meningeal gliomatosis, 2/29 (7%) of the cases showed alteration of the PTEN gene. In contrast, 5/15 (33%) of malignant gliomas with meningeal gliomatosis cases showed this alteration. These findings indicate that PTEN gene mutation contributes not only to the neoplastic evolution in gliomas but also to the meningeal dissemination of glioma cells.


Assuntos
Neoplasias Encefálicas/genética , Glioma/genética , Neoplasias Meníngeas/genética , Mutação/genética , Monoéster Fosfórico Hidrolases/genética , Proteínas Supressoras de Tumor/genética , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Análise Mutacional de DNA , Enzimas de Restrição do DNA/metabolismo , DNA de Neoplasias/genética , Feminino , Genes Supressores de Tumor , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , PTEN Fosfo-Hidrolase , Polimorfismo Conformacional de Fita Simples
12.
J Dermatol Sci ; 26(3): 217-32, 2001 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-11390207

RESUMO

This study was conducted to determine whether exposing mice to ultraviolet (UV) radiation would alter the pathogenesis of infection with Leishmania (Leishmania) amazonensis (L. amazonensis) which causes progressive cutaneous disease in susceptible mouse strains. BALB/c mice were irradiated with 10 and 30 J/cm(2) UVA on shaved skin of the back from Dermaray (M-DMR-100) for 4 consecutive days before infection with Leishmania promastigotes. The course of disease was recorded by measuring the size of lesions at various times after infection. Mice groups irradiated with UVA 10 and 30 J/cm(2) showed significantly suppressed lesion development compared with the non-irradiated mice. Light and electron microscopy revealed a few parasites at the site of inoculation in UVA-irradiated subjects. Sandwich enzyme-linked-immunosorbent-assay (ELISA) examination of sera showed dose dependently upregulated interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL)-12, and downregulated interleukin (IL)-4 and interleukin (IL)-10 levels in UVA-irradiated as compared with the non-irradiated mice. Positive signals for IFN-gamma mRNA in irradiated mice were obtained by RT-PCR, while non-irradiated mice showed negative results. None of the examined samples showed signal for IL-4 mRNA. The present study disclosed that exposure of mice to different low-doses of UVA irradiation prior to infection may interfere with immunity to L. amazonensis in the murine model. This indicates that the cell-mediated response switch from Th2 to Th1 pattern suppressed the cutaneous lesions of L. amazonensis.


Assuntos
Leishmaniose/imunologia , Leishmaniose/patologia , Células Th1/fisiologia , Células Th1/efeitos da radiação , Raios Ultravioleta , Animais , Relação Dose-Resposta à Radiação , Regulação para Baixo , Sistema Imunitário/efeitos da radiação , Interferon gama/genética , Interferon gama/metabolismo , Interleucina-10/metabolismo , Interleucina-12/metabolismo , Interleucina-4/genética , Interleucina-4/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Microscopia Eletrônica , RNA Mensageiro/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Regulação para Cima
13.
Neurosurg Rev ; 24(1): 48-52, 2001 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11339470

RESUMO

A 22-year-old woman presented with acute onset of headache and vomiting. Computed tomography (CT) demonstrated hydrocephalus and a huge midline mass with heterogeneous density involving both lateral ventricles. A small amount of hematoma was detected at the bottom of the left trigone. On magnetic resonance imaging (MRI), the mass appeared grossly isointense on T1-weighted images and slightly hyperintense on T2-weighted images with a clearly demarcated low intensity area at its center. These CT and MRI findings were suggestive of an acute hemorrhagic event within the tumor. The presence of hemorrhage was confirmed at surgery. Sudden hemorrhages within the tumor were considered to cause the acute onset of symptoms. Although central neurocytoma is not commonly known as a tumor-producing intracranial hemorrhage or to cause abrupt clinical deterioration, we found five similar cases in the literature. After reviewing these cases, we concluded that the information on the possible hemorrhagic complication of central neurocytoma is important for correct diagnosis and thus for proper management of this tumor.


Assuntos
Hemorragia Cerebral/cirurgia , Neoplasias do Ventrículo Cerebral/cirurgia , Neurocitoma/cirurgia , Adulto , Hemorragia Cerebral/diagnóstico , Hemorragia Cerebral/patologia , Neoplasias do Ventrículo Cerebral/diagnóstico , Neoplasias do Ventrículo Cerebral/patologia , Ventrículos Cerebrais/patologia , Ventrículos Cerebrais/cirurgia , Diagnóstico Diferencial , Feminino , Humanos , Imageamento por Ressonância Magnética , Neurocitoma/diagnóstico , Neurocitoma/patologia , Tomografia Computadorizada por Raios X
14.
Blood ; 97(10): 2972-8, 2001 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-11342419

RESUMO

The properties of 9 delta-aminolevulinate dehydratase (ALAD) mutants from patients with ALAD porphyria (ADP) were examined by bacterial expression of their complementary DNAs and by enzymologic and immunologic assays. ALADs were expressed as glutathione-S-transferase (GST) fusion proteins in Escherichia coli and purified by glutathione-affinity column chromatography. The GST-ALAD fusion proteins were recognized by anti-ALAD antibodies and were enzymatically active as ALAD. The enzymatic activities of 3 ALAD mutants, K59N, A274T, and V153M, were 69.9%, 19.3%, and 41.0% of that of the wild-type ALAD, respectively, whereas 6 mutants, G133R, K59N/G133R, F12L, R240W, V275M, and delTC, showed little activity (< 8%). These variations generally reflect the phenotype of ALAD in vivo in patients with ADP and indicate that GST-ALAD fusion protein is indeed useful for predicting of the phenotype of ALAD mutants. The location of F12L mutation in the enzyme's molecular structure indicates that its disturbance of the quaternary contact of the ALAD dimer appears to have a significant influence on the enzymatic activity. Mouse monoclonal antibodies to human ALAD were developed that specifically recognized a carboxy terminal portion of ALAD, or other regions in the enzyme. This study represents the first complete analysis of 9 mutants of ALAD identified in ADP and indicates the highly heterogeneous nature of mutations in this disorder.


Assuntos
Mutação , Sintase do Porfobilinogênio/deficiência , Sintase do Porfobilinogênio/genética , Porfirias/enzimologia , Adolescente , Animais , Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos , Escherichia coli/genética , Feminino , Expressão Gênica , Glutationa Transferase/genética , Humanos , Immunoblotting , Recém-Nascido , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Pessoa de Meia-Idade , Fenótipo , Sintase do Porfobilinogênio/metabolismo , Porfirias/genética , Proteínas Recombinantes de Fusão/análise , Proteínas Recombinantes de Fusão/isolamento & purificação , Proteínas Recombinantes de Fusão/metabolismo
15.
Am J Hematol ; 65(3): 234-8, 2000 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11074541

RESUMO

The first case of B-cell lymphoma of brain in a patient with myelodysplastic syndrome (MDS) was reported. A 68-year-old man was admitted because of anemia, fever, and thrombocytopenia and was diagnosed as having MDS (refractory anemia with excess of blasts) on the basis of the findings of bone marrow aspiration and chromosomal analysis. The patient was followed up without chemotherapy, but a brain tumor appeared after 3 years. Histologic and immunohistologic examinations revealed diffuse large B-cell lymphoma. Mutations of the c-kit proto-oncogene (stem cell factor receptor) and the p53 tumor-suppressor gene were examined in the MDS lesion and malignant lymphoma (ML) by the polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) method followed by direct sequencing. The p53 mutation was not found in either MDS or ML, but a nonsense mutation (Try-557 --> stop) in exon 11 of the c-kit, which might lead to dysfunction of tyrosine kinase activity, was detected in MDS. This is the first report of c-kit mutation in MDS. Epstein-Barr virus (EBV) genome was demonstrated in the nucleus of brain ML cells by in situ hybridization with EBV-encoded RNA-1 probe. Immunohistochemistry showed that the tumor cells expressed latent infection gene products, including EBV nuclear antigen-2 and latent membrane protein-1. This pattern of latent gene expression was Lat III, which is usually found in malignant lymphomas developing in immunocompromised hosts. These findings suggest that a profound pancytopenia in MDS resulted in an immunodeficient condition, after which EBV-positive B-cell lymphoma of brain developed.


Assuntos
Neoplasias Encefálicas/etiologia , Linfoma de Células B/etiologia , Síndromes Mielodisplásicas/complicações , Proteínas Proto-Oncogênicas c-kit/genética , Idoso , Linfoma de Burkitt/genética , Genes p53/genética , Humanos , Imuno-Histoquímica , Hibridização In Situ , Masculino , Mutação Puntual , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita Simples , Proto-Oncogene Mas
16.
Am J Dermatopathol ; 22(5): 447-52, 2000 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11048983

RESUMO

We report a case of verruciform xanthoma (VX) associated with human papillomavirus (HPV) in a 67-year-old male. The patient had a pale-reddish, granular and verrucous tumor on the right side of his scrotum for four years. Histopathologic examination showed typical features of VX. HPV was detected by immunohistochemistry, electron microscopy, and PCR examinations. Ultrastructural examination revealed virus-like particles of 40-50 nm in the nucleus of the upper epidermal keratinocytes. HPV type 6a DNA was detected in lesional tissue by polymerase chain reaction and sequence analysis. To the best of our knowledge, this is the first case report of VX associated with HPV.


Assuntos
Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/virologia , Doenças Testiculares/virologia , Infecções Tumorais por Vírus/virologia , Xantomatose/virologia , Idoso , Sequência de Bases , DNA Viral/análise , Humanos , Masculino , Dados de Sequência Molecular , Papillomaviridae/genética , Papillomaviridae/ultraestrutura , Infecções por Papillomavirus/patologia , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico , Doenças Testiculares/patologia , Infecções Tumorais por Vírus/patologia , Xantomatose/patologia
18.
19.
Brain Tumor Pathol ; 17(1): 21-7, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10982006

RESUMO

Frozen sections of 35 astrocytic tumors of various histologic malignancies were analyzed by comparative genomic hybridization in an attempt to characterize the profile of genetic aberrations. Over 94% of the samples revealed DNA copy number aberrations, which increased with higher histological malignancy grades, and also involvement of more than one chromosome was seen in 85% of instances. The aberrations observed were mainly deletions and most frequently incorporated chromosomes 1p, 10, 19q, and 22q. On the other hand, gains or amplifications were detected only in glioblastomas. Additionally, such gains or amplifications were present in all tumor samples where the initial histopathological diagnosis was glioblastoma and immunohistochemical study disclosed p53 tumor suppressor protein negative and epidermal growth factor receptor positive immunoreactivity; such glioblastomas possessing p53 tumor suppressor protein positive and epidermal growth factor receptor negative immunoreactivity seldom displayed any gain. Thus, glioblastomas exhibiting two different profiles of genetic aberrations were recognized--one with and the other without any gains/ amplifications. We speculate that the former variety is de novo glioblastoma.


Assuntos
Astrocitoma/genética , Neoplasias Encefálicas/genética , Genoma , Glioblastoma/genética , Adulto , Idoso , DNA/genética , Receptores ErbB/metabolismo , Amplificação de Genes , Deleção de Genes , Dosagem de Genes , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade , Hibridização de Ácido Nucleico , Proteína Supressora de Tumor p53/metabolismo
20.
Neurol Res ; 22(5): 478-80, 2000 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-10935220

RESUMO

Choroid plexus carcinoma in an adult case is a very rare tumor and difficult to differentiate from metastatic tumors. The authors report a case of a 49-year-old female with choroid plexus carcinoma who previously had multiple carcinomas. In this case, synaptophysin immunohistochemistry proved to be extremely helpful for the histological diagnosis.


Assuntos
Carcinoma/diagnóstico , Carcinoma/metabolismo , Neoplasias do Plexo Corióideo/diagnóstico , Neoplasias do Plexo Corióideo/metabolismo , Sinaptofisina/metabolismo , Carcinoma/patologia , Carcinoma/cirurgia , Neoplasias do Plexo Corióideo/patologia , Neoplasias do Plexo Corióideo/cirurgia , Feminino , Humanos , Imuno-Histoquímica , Imageamento por Ressonância Magnética , Pessoa de Meia-Idade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...