4-({[(E)-Pyridin-3-yl-methyl-idene]amino}-meth-yl)cyclo-hexa-necarb-oxy-lic acid.

The title compound, C(14)H(18)N(2)O(2), contains two geometrically different mol-ecules in the asymmetric unit: the basal plane of the cyclo-hexane chair and the N-[pyridin-3-yl-methyl-idene]methanamine moiety are oriented at dihedral angles of 71.77 (7)° and 83.42 (8)°. In the crystal, the mol-ecules are linked by O-H⋯N hydrogen bonds, generating C(13) head-to-tail chains extending along the base vector [103]. R(2) (2)(26) ring motifs are formed due to the C-H⋯·O inter-actions that link neighbouring chains. There also exist π-π inter-actions [centroid-centroid separation = 3.6925 (12) Å] between the symmetry-related pyridine rings of one of the independent mol-ecules.

Antibacterial, antifungal, insecticidal, cytotoxicity and phytotoxicity studies on Indigofera gerardiana.

The antibacterial, antifungal, acute cytotoxicity, phytotoxicity and insecticidal profile of the crude extract and various fractions of Indigofera gerardiana have been studied. Six bacterial and fungal strains were used, of which Samonella typhi and Microsporum canis were the most susceptible strains with MICs 0.37 mg/mL and 0.09 mg/mL, respectively. The crude extract and the fractions showed low insecticidal activity against Sitophilus oryzae, Rhyzopertha dominica and Callosbruchus analis but no activity against Tribolium castaneum. The brine shrimp lethality assay showed absence of any measurable cytotoxicity of the crude extract and fractions, showing a good safety profile at a preliminary level. All the fractions except crude extract revealed profound and highly significant herbicidal activity against Lemna minor at the concentration of 1000 microg/mL. Indigofera gerardiana was shown by in-vitro assays to be a potential source for natural antifungal, antibacterial and herbicidal agents.