1.
Biotechnol Bioeng
; 57(2): 216-9, 1998 Jan 20.
Artigo
em Inglês
| MEDLINE
| ID: mdl-10099196
RESUMO
Liposomes were prepared from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), which contained the water soluble proteinase alpha-chymotrypsin. This liposome entrapped enzyme showed selectivity for externally added substrates in that only small substrates (benzoyl-l-Tyr-p-nitroanilide or acetyl-l-Phe-p-nitro-anilide)-for which the liposome bilayer was permeable-were transformed into products. Large substrates (succinyl-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide or casein) could not penetrate from the external aqueous phase into the liposomes, and were not hydrolyzed. This substrate selectivity is entirely based on the compartimentation and permeability properties of the liposome microreactor.
Assuntos
Quimotripsina/metabolismo , Biotecnologia , Caseínas , Quimotripsina/química , Estabilidade Enzimática , Enzimas Imobilizadas , Técnicas In Vitro , Cinética , Bicamadas Lipídicas/química , Lipossomos , Permeabilidade , Fosfatidilcolinas , Especificidade por Substrato
2.
J Chronic Dis
; 27(3): 163-9, 1974 Mar.
Artigo
em Inglês
| MEDLINE
| ID: mdl-4830843
3.
Lancet
; 1(7852): 289-91, 1974 Feb 23.
Artigo
em Inglês
| MEDLINE
| ID: mdl-4130474