Investigation and Assessment for an effective approach to the reclamation of Polycyclic Aromatic Hydrocarbon (PAHs) contaminated site: SIN Bagnoli, Italy.

Native plant species were screened for their remediation potential for the removal of Polycyclic Aromatic Hydrocarbons (PAHs) contaminated soil of Bagnoli brownfield site (Southern Italy). Soils at this site contain all of the PAHs congeners at concentration levels well above the contamination threshold limits established by Italian environmental legislation for residential/recreational land use, which represent the remediation target. The concentration of 13 High Molecular Weight Polycyclic Aromatic Hydrocarbons in soil rhizosphere, plants roots and plants leaves was assessed in order to evaluate native plants suitability for a gentle remediation of the study area. Analysis of soil microorganisms are provides important knowledge about bioremediation approach. Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria are the main phyla of bacteria observed in polluted soil. Functional metagenomics showed changes in dioxygenases, laccase, protocatechuate, and benzoate-degrading enzyme genes. Indolacetic acid production, siderophores release, exopolysaccharides production and ammonia production are the key for the selection of the rhizosphere bacterial population. Our data demonstrated that the natural plant-bacteria partnership is the best strategy for the remediation of a PAHs-contaminated soil.

Data matrix of site-specific environmental variables: Phytomanagement of a contaminated brownfield site.

This article offers statistical analyses of trace elements (TEs) in soils and plants through a Pearson correlation matrix. The main objectives were the assessment of soil TEs sources and the evaluation of native plant response to physical and chemical characteristics of a TEs contaminated soil. Data were collected from Bagnoli brownfield site (Southern Italy). Interpretation of the data, can be found in "Identification of native-metal tolerant plant species in-situ: environmental implications and functional traits" [1]. The correlations in the matrix are based on over 76 samples and 31 site-specific environmental variables.

Molecular and environmental analysis of Campania (Italy) sweet cherry (Prunus avium L.) cultivars for biocultural refugia identification and conservation.

Conservation of agrobiodiversity is a major concern worldwide. Several strategies have been designed and programmed to reduce biodiversity erosion due to anthropic and non-anthropic causes. To this end, we set up a multidisciplinary approach based on the genetic analysis of selected cultivars and recognition of the environmental parameters. We genotyped the sweet cherry cultivars of Campania region in southern Italy by using simple sequence repeats and further investigated them by cluster analysis, disclosing a homogeneous genetic constitution, different from that of commercial accessions. By structure analysis we identified three distinct genetic clusters, each characterized by common and distinct alleles. Survey of the cultivars' geographical distribution by quartic kernel function identified four preferred districts further characterized for soil origin, pedologic, agronomic features and urbanization impact. We correlated these environmental parameters, typical of the identified areas, with the three genetic pools and found a statistically significant association for each cluster. When we overlaid the cultivation traditions and cultural heritage, we found they have a dominant role; on these premises, we generated new territorial maps. In conclusion, we propose a novel methodological approach based on molecular, geo-pedological and cultural parameters with the aim to recognize biocultural refugia and preserve endangered or valuable cultivars.

Identification of native-metal tolerant plant species in situ: Environmental implications and functional traits.

A study was undertaken to identify suitable native plants for the phytoremediation of the second largest integrated steelworks in Italy (Bagnoli brownfield site). A phytoecological survey allowed us to identify 139 plant taxa belonging to 58 different families. The most represented families were in the decreasing order Poaceae > Fabaceae > Asteraceae > Apiaceae. The biological spectrum showed a predominance of Therophytes > Hemycriptophytes > Phanerophyte. Seventy-six sites were selected on the presence of colonist's plants and vegetation assemblage patterns. At each site, roots and leaves of the dominant plant and rhizosphere soils were sampled. Total content of metal(loid)s in soils and plant parts were determined. Agronomic soil parameters were studied. Anthropogenic sourced metal(loid)s were discriminated from geochemical ones, and plant metal(loid) accumulation and translocation efficiency were evaluated. The role of many native plant species in terms of TEs phytomanagement strategy was recognizable inside the investigated area. According to this survey of structural plant diversity, several combinations between plants and microorganisms are being further investigated to identify relevant biological system for the phytomanagement of this contaminated area.