RESUMO
A combined experimental and theoretical approach has been used to disentangle the fundamental mechanisms of the fragmentation of the three isomers of nitroimidazole induced by vacuum ultra-violet (VUV) radiation, namely, 4-, 5-, and 2-nitroimidazole. The results of mass spectrometry as well as photoelectron-photoion coincidence spectroscopy display striking differences in the radiation-induced decomposition of the different nitroimidazole radical cations. Based on density functional theory (DFT) calculations, a model is proposed which fully explains such differences, and reveals the subtle fragmentation mechanisms leading to the release of neutral species like NO, CO, and HCN. Such species have a profound impact in biological media and may play a fundamental role in radiosensitising mechanisms during radiotherapy.
Assuntos
Nitroimidazóis/química , Processos Fotoquímicos , Isomerismo , Modelos Moleculares , Conformação Molecular , Teoria QuânticaRESUMO
Allele frequencies of 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 163 unrelated individuals from the Republic of Macedonia. AmpFISTR Identifiler Kit (Applied Biosystems) was used for PCR amplification. For all 15 loci, the combined matching chance is 6.6 x 10(18) and the power of exclusion is 99.999954%.
Assuntos
Frequência do Gene , Genética Populacional , Sequências de Repetição em Tandem , Impressões Digitais de DNA , Humanos , Reação em Cadeia da Polimerase , República da Macedônia do NorteRESUMO
Sera of 1255 individuals from Novi Sad, varying in age from less than 1 month to 69 years, have been tested for neutralization antibodies to Herpes implex virus type 1. The eight newborns tested and 97% of the 507 adults were positive, with titres ranging from 1/4 to 1/256. The titres in newborns were significantly lower than the titres in adults. After birth the maternal antibodies declined rapidly and 94% of infants at the age of greater than 6 months and less than 2 years were negative. After the first year infants in Novi Sad start to acquire herpes-neutralizing antibodies actively, reaching a 50% incidence of positives between the 2nd and 3rd year of age. Age-specific incidence rates of herpes positives found in Novi Sad have been compared with those reported from Edinburgh, Freiburg i. Br. and Louisiana. Possible influences of several circumstances upon the incidence rate of positives detected by the neutralization test are discussed.
Assuntos
Anticorpos Antivirais/análise , Simplexvirus/imunologia , Adolescente , Adulto , Fatores Etários , Idoso , Criança , Pré-Escolar , Alemanha Ocidental , Humanos , Lactente , Recém-Nascido , Louisiana , Pessoa de Meia-Idade , Testes de Neutralização , Reino Unido , IugosláviaRESUMO
In the province of Voyvodina 18 cases of post-vaccinal encephalitis have been recorded, three of which were fatal.The estimated morbidity rate was 0.6 per 10(5) after revaccination, and 3.9 per 10(5) after primary vaccination. The virological studies described, as well as other findings referred to in this report, are consistent with the possibility of an autoimmune aetiology of the post-vaccinal encephalitis cases observed.