The role of microglia in early neurodevelopment and the effects of maternal immune activation.

Activation of the maternal immune system during gestation has been associated with an increased risk for neurodevelopmental disorders in the offspring, particularly schizophrenia and autism spectrum disorder. Microglia, the tissue-resident macrophages of the central nervous system, are implicated as potential mediators of this increased risk. Early in development, microglia start populating the embryonic central nervous system and in addition to their traditional role as immune responders under homeostatic conditions, microglia are also intricately involved in various early neurodevelopmental processes. The timing of immune activation may interfere with microglia functioning during early neurodevelopment, potentially leading to long-term consequences in postnatal life. In this review we will discuss the involvement of microglia in brain development during the prenatal and early postnatal stages of life, while also examining the effects of maternal immune activation on microglia and neurodevelopmental processes. Additionally, we discuss recent single cell RNA-sequencing studies focusing on microglia during prenatal development, and hypothesize how early life microglial priming, potentially through epigenetic reprogramming, may be related to neurodevelopmental disorders.

Assessing anterior cruciate ligament injuries: the association and differential value of questionnaires, clinical tests, and functional tests.

It is important to examine the package of questionnaires and clinical and functional tests as used in anterior cruciate ligament (ACL)-injured patients in order to gain insight on the patient's present status. Nine measuring systems in three categories were examined: four questionnaires, three clinical tests, and two functional tests. Differences between sports activity rating system, factor occupational rating system scale, and Tegner scores pre- and post-injury and the differences between the affected and unaffected knee in the clinical and functional tests were calculated using the Wilcoxon test for paired observations. These differences proved to be significant (p < 0.05). The association between the various tests was also examined. None of the associations satisfied the preset standards. Based on these low levels of association, it does not seem possible to reduce the package of tests to one questionnaire, one clinical test, and one functional test as all questionnaires and tests seem to be related to different aspects of the injured ACL. Based upon these results, the total package should be used to gain insight in both impairment and disability level in patients with an injured ACL.