A study to evaluate the effectiveness of various disinfectants for extracted human teeth - A comparative study.

Objectives: The aim of this study was to evaluate the effectiveness of various disinfectants for extracting human teeth. The objectives were to assess the effectiveness of 5% vinegar, 3% hydrogen peroxide, 70% alcohol, and 5.25% sodium hypochlorite for disinfection of extracted human teeth. Methods: The teeth were divided into one of the following four groups consisting of 10 teeth in each group. All teeth were immersed in separate bottles containing 10 ml of the disinfectant for 7 days at 25°C. No visible growth in the broth was considered effective disinfection. The Institutional Ethics Committee (IEC) Number is SVIEC/ON/DENT/SRP/15186, and the IEC Clearance number is SVIEC/ON/DENT/SRP/15040. Results: Vinegar was most effective for bacterial disinfection, and 5.25% sodium hypochlorite was most effective for fungal disinfection. The result was statistically significant with Chi-square values of 0.015 and 0.057, respectively, and P < 0.001. Conclusions: Extracted teeth should be handled with extreme care as these are a potential source of infection. The present study documented the role of various disinfectants. Vinegar can be used as an effective disinfectant medium for extracting human teeth. Sterilisation and autoclaving are superior options while vinegar is cheap, readily available, and relatively non-toxic. All these qualities plus the fact that it has given encouraging results as an antibacterial disinfectant should necessitate its usage on a more regular basis than it is used at present.

Effect of Octreotide in stopping post surgical chyle leak in neck Dissection-A systematic review.

Objective: Neck dissection causes an unusual and challenging complication called Chyle Leak. Octreotide, a long-acting somatostatin analogue, is one way Chyle Leak can be managed faster and effectively. The objective of the review was to evaluate the efficiency of Octreotide in stopping post surgical Chyle Leak in neck dissection. Methods: Three electronic database and manual search was undertaken to identify the literature. All the published articles, which included data about Octreotide used to stop post-surgical Chyle leak in neck dissection, published in the English language between January 1, 2010 to May 31, 2022 were included. Joanna Briggs critical assessment tool was used to assess the included studies. Results: Preliminary screening of 206 studies from data sources and ten from additional sources was done. After necessary exclusion, ten studies were included for qualitative synthesis. The data included 65 patients with neck dissections followed by Chyle leak postoperatively. Chyle leak was presented from 0-8th POD (range of 150 ml-2500 ml). 100mcg-eight hourly subcutaneously was given in maximum studies for 2-14 days. A gradual reduction in chyle leak started 2-4 days after the administration. The chyle leak completely resolved within 2-11 days in the majority of cases with conservative treatment and Octreotide. Conclusion: Octreotide Therapy with primary treatment is an effective way of stopping Chyle Leak in Neck Dissection. However, well-designed and robust randomized controlled studies are needed to confirm the results in the future.

Staining the Ground Section of the Tooth Using an Innovative Plant Stain Found in the Pravara Region, India.

AIMS: To assess the staining efficacy of the innovative "Pravara" stain on ground sections of human teeth under a stereomicroscope. In this study, the ground sections were stained with the innovative stain. The stain is obtained from a leafless tree called Terminalia chebula. Terminalia chebula, commonly known as Harad, is found in South Asia from India. Few species are found in the Pravara region of Maharashtra, India. MATERIALS AND METHODS: A total of 40 extracted teeth were collected and ground sections of each tooth were prepared. The samples were randomly divided into two groups: group I-control (without stain), group II-study group (the samples in this group were stained with the help of innovative "Pravara" stain). The sections were graded from I to III according to the differentiation and contrast of tissues. RESULTS: The ground sections in group II stained with Pravara stain demonstrated better contrast for structures within sections under a microscope in comparison with group I (control group). CONCLUSION: The study demonstrates the efficacy of a new innovative Pravara stain, which is a simple, economical, quick, and effective staining method for the ground sections of the teeth. CLINICAL SIGNIFICANCE: This innovative Pravara stain is cost-effective, saves time, ecofriendly, and highlights the anatomical structures more effectively and does not fade easily with time.