RESUMO
Two novel 11(9 â 7)-abeo-ergostane-type steroids, named pleurocins A (1) and B (2), a 13,14-seco-13,14-epoxy ergostane, named eringiacetal B (3), and an ergostane steroid (4) were isolated from the fruiting bodies of Pleurotus eryngii (Pleurotaceae). Their structures were determined by spectroscopic data and X-ray crystallography. A possible biogenesis pathway for 1-3 was also described. Compounds 1-3 exhibited inhibitory activities against NO production with almost no cytotoxicity at concentrations lower than 30 µM.
Assuntos
Basidiomycota/química , Ergosterol/análogos & derivados , Carpóforos/química , Óxido Nítrico/antagonistas & inibidores , Animais , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Cristalografia por Raios X , Relação Dose-Resposta a Droga , Ergosterol/química , Ergosterol/isolamento & purificação , Ergosterol/farmacologia , Camundongos , Modelos Moleculares , Conformação Molecular , Óxido Nítrico/biossíntese , Células RAW 264.7 , Relação Estrutura-AtividadeRESUMO
Six new ergostane-type steroids; (22E)-3ß,5α,6α,11-tetrahydroxy-9(11)-seco-ergosta-7,22-dien-9-one (1), (22E)-8,14-epoxyergosta-6,22-diene-3ß,5α,9α-triol (2), (22E)-4α,5α-epoxyergosta-7,22-diene-3ß,6ß-diol (3), (22E)-3ß,4ß,5α-trihydroxyergosta-7,22-dien-6-one (4), (22E)-ergosta-7,22-diene-3ß,5ß,6α-triol (5), and (22E)-6ß-methoxyergosta-7,22-diene-3ß,5α-diol 3-O-ß-d-glucopyranoside (6) were isolated from the fruiting bodies of king trumpet mushroom (Pleurotus eryngii), along with fourteen known compounds (7-20). All isolated compounds were evaluated for their inhibitory effects on macrophage activation using a nitric oxide production inhibition assay.
