Amino acid availability of protein meals of different quality for adult and growing mink (Neovison vison).

Protein and amino acid (AA) availability of three protein meals of expected different quality were evaluated in young growing and adult mink. Lamb meal (LM), poultry meal (PM) or fishmeal (FM) were used as main protein sources in three extruded diets investigated by determining apparent total tract digestibility (ATTD) and nitrogen balance in 12 growing mink males aged 8-11 weeks in a Latin square design. In adult mink, ATTD of the diets was determined. The diets had lower protein content than recommended for growing mink, protein contributing 23% of total metabolisable energy (ME), to ensure differences in growth response. The LM diet with expected low protein quality revealed lower content of essential AA than the PM and FM diets. The ATTD of major nutrients and essential AA was significantly affected by diet, with the poorest values for LM, intermediate for PM and the highest values for FM. Mink kits revealed lower ATTD values than adults for protein, AA and especially fat, resulting in lower dietary ME content for kits than for adults. The mean difference was greatest for the LM diet with lowest ATTD and smallest for the FM diet with the highest ATTD. Nitrogen retention and growth rate differed significantly among diets and was lowest for diet LM and highest for diet FM, respectively. Different dietary essential AA contents and ATTD, especially of methionine, were the main factors to explain the difference in growth response. Generally, plasma essential AA concentrations did not clearly reflect the different dietary supply and the different growth response. The study shows that a recommended level of 0.31 g apparent total tract digestible methionine per MJ ME covers the minimum requirement with a safety margin. To obtain optimal growth, the lower digestive capacity in young mink kits should be considered when choosing feed ingredients.

Foetal life protein provision of mink (Neovison vison) changes the relative mRNA abundance of some hepatic enzymes regulating fat metabolism.

The nutrient provision to pregnant females has high impact on the growth and metabolism of their offspring. The objective was to investigate if the expression of hepatic enzymes regulating the fat metabolism was affected in foetuses and adult female mink born by dams fed either a low or an adequate level of protein during late gestation. The relative abundances of acetyl coenzyme A carboxylase (ACC), fatty acid synthase (FAS) and carnitine palmitoyl transferase 1 (CPT1) mRNA were determined by qualitative polymerase chain reaction in the livers of F0- and F1-generation dams and in F1-generation foetuses. Low protein provision during foetal life resulted in a lower expression of FAS in foetal liver but a tendency towards increased expression in the liver of adult dams. There was a tendency towards an effect of life stage of the animal on the expression of ACC resulting in a higher expression among F1 foetuses exposed to low protein during foetal life than F0 dams fed a low protein diet during late gestation. The expression of CPT1 was significantly lower among dams exposed to low protein provision during foetal life than controls, possibly indicating a lower rate of mitochondrial ß-oxidation. Further investigations are needed to clarify the consequences of these changes for the fat metabolism.

Metabolic and growth response of mink (Neovison vison) kits until 10 weeks of age when exposed to different dietary protein provision.

Growth performance and metabolism were investigated in mink kits (n = 210) exposed to the same dietary treatment as their dams (n = 30), i.e. high (HP; 61% of metabolisable energy, ME), medium (MP; 48% of ME) or low (LP; 30% of ME) protein supply, from birth until 10 weeks of age. The kits were weighed weekly, and were measured by means of balance experiment and indirect calorimetry, in weeks eight and nine post-partum (p.p.). At weaning (seven weeks p.p.) and 10 weeks p.p. one kit per litter was killed and blood, liver and kidneys were collected. Plasma amino acid profiles, and hepatic abundance of mRNA for phosphoenolpyruvate carboxykinase (PEPCK), fructose 1,6-biphosphatase, pyruvate kinase and glucose-6-phosphatase (G-6-Pase) by q-PCR, were determined. There were no differences in live weights among kits the first four weeks of life when kits solely consumed milk, but male LP kits were the heaviest. After transition to solid feed MP kits weighed most at nine weeks of age (p < 0.05). At eight weeks of age, the kits fed the LP diet retained less (p < 0.05) N than HP and MP kits. Heat production did not differ among kits, although protein oxidation was higher (p < 0.001) in HP kits than in LP kits. Kits fed the LP diet had lower (p < 0.05) plasma concentrations of lysine, methionine and leucine than MP kits. Dietary treatment was not reflected in the relative abundance of any of the studied mRNAs, but kits had significantly lower abundance of all studied mRNA than their dams, ranging from 83% less PEPCK abundance to 40% less for G-6-Pase. The kidney mass was smallest (p < 0.01) in kits fed the LP diet, and liver masses were largest (p < 0.001) in HP kits. The results indicate that the LP diet did not meet the protein requirements for mink kits in the transition period from milk to solid feed. The capacity to regulate the rate of gluconeogenesis was even more limited in young mink kits than in adult dams. However, young mink kits can regulate protein oxidation in response to dietary protein supply, probably by adapting the size of the liver and kidneys to the level of protein supply.

Effect of late gestation low protein supply to mink (Mustela vison) dams on reproductive performance and metabolism of dam and offspring.

Protein malnutrition in utero that induces permanent changes in metabolism has been investigated intensively in various animals in recent years, but to the best of our knowledge, not yet in the mink, a strict carnivore. In the present study, minks were fed either a low-protein (LP) diet, i.e., with a protein:fat:carbohydrate ratio of 14:51:35% of metabolisable energy (ME), or an adequate-protein diet (AP), i.e. 29:56:15% of ME, from when implantation was completed until parturition (17.9 +/- 3.6 days). Respiration and balance experiments were performed during both gestation and lactation. Plasma concentrations of leptin, IGF-1, and insulin were determined by radioimmunoassay; the relative abundances of glucose-6-phosphatase (G-6-Pase), fructose-1,6-bisphosphatase (Fru-1,6-P2ase), phosphoenol-pyruvate carboxykinase (PEPCK), and pyruvate kinase (PKM2) were determined in liver, and abundances of adiponectin and leptin in adipose tissue were determined by real-time quantitative PCR (q PCR). The protein supply only affected quantitative metabolism traits during the period of differentiated feeding. The dietary composition was reflected in the nitrogen metabolism and substrate oxidation, but no effects remained during lactation. The LP dams tended to have a smaller liver mass in relation to body weight than did AP dams (2.5% vs. 2.9%; p = 0.09), significantly less leptin mRNA (p < 0.05), and 30.6% fewer kits per mated female (p = 0.03). Furthermore, F1-generation kits exposed to protein restriction during foetal life (FLP1; 10.3 g) had a lower birth weight (p = 0.004) than did F1-generation kits exposed to adequate protein (FAP1; 11.3 g). Differences remained significant until 21 days of age (120.4 g vs. 127.6 g; p = 0.005). The FLP1 foetuses displayed a lower abundance of Fru-1,6-P2ase mRNA (p = 0.007) and of PKM2 mRNA (p = 0.002) than did FAP1 foetuses. Whether these changes during foetal life cause permanent changes in the glucose homeostasis of the offspring and result in the transmission of epigenetic phenotypic changes, as seen in the rat, needs further investigation.