RESUMO
Novel N-substituted tetrahydro-2,4-dioxoquinazolin-1-yl acetic acids characterized by formal replacement of the substituted benzyl moiety by cyclohexylmethyl and n-heptyl residues, respectively, were synthesized and evaluated as aldose reductase inhibitors.
Assuntos
Acetatos/síntese química , Acetatos/farmacologia , Aldeído Redutase/antagonistas & inibidores , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/farmacologia , Quinazolinas/síntese química , Quinazolinas/farmacologia , Animais , Bovinos , Dimetil Sulfóxido , Cristalino/enzimologia , Espectroscopia de Ressonância Magnética , Solventes , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
Starting from substituted 3,6-dichloropyridazine-4-carboxamides (2, 3) tri- and tetracyclic compounds (4, 5) could be smoothly prepared. Structural modifications of interest with regard to biological activity were performed by N-alkylation and reductive dehalogenation. The new substituted heterocyclic compounds were screened as antimycobacterial agents; the influence of the substitution pattern on activity is discussed.
Assuntos
Piridazinas/síntese química , Piridazinas/farmacologia , Antibacterianos/síntese química , Antibacterianos/química , Antibacterianos/farmacologia , Antituberculosos/síntese química , Antituberculosos/química , Antituberculosos/farmacologia , Compostos Heterocíclicos/síntese química , Compostos Heterocíclicos/química , Compostos Heterocíclicos/farmacologia , Lipídeos/química , Lipídeos/farmacologia , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Estrutura Molecular , Mycobacterium tuberculosis/efeitos dos fármacos , Piridazinas/química , Relação Estrutura-AtividadeRESUMO
Starting from 3-(3-chloro-1H-pyrazol-5-yl)-1H-quinoxalin-2-one (2) a series of substituted [1,2,4]triazolo[4,3-a]quinoxalines (3a-f) was prepared via a multistep reaction sequence. Affinities of the novel derivatives 3a-f for benzodiazepine as well as for adenosine A1- and A2A-receptors of rat brain were determined by radioligand binding assays. 1-Methyl-4-(3-chloro-1H-pyrazol-5-yl) derivative 3a exhibited submicromolar affinity for the benzodiazepine binding site of GABAA receptors (Ki = 340 nM) and was less potent at A1-(Ki = 7.85 microM) and A2A-(Ki = 1.43 microM) adenosine receptors (AR). Derivatives with larger substituents in the 1-position showed reduced binding to benzodiazepine and A2A-AR, but increased A1-AR affinity, the 2-thienylmethyl derivative 3f being the most potent and selective A1-AR ligand of the present series (Ki = 200 nM).
Assuntos
Antagonistas de Receptores de GABA-A , Antagonistas de Receptores Purinérgicos P1 , Pirazóis/síntese química , Quinoxalinas/síntese química , Triazóis/síntese química , Animais , Ligantes , Pirazóis/farmacologia , Quinoxalinas/farmacologia , Ratos , Relação Estrutura-Atividade , Triazóis/farmacologiaRESUMO
Starting from amino(di)azines and 2-chloro-6-methyliso(thio)cyanate a series of aryl-substituted urea and thiourea derivatives was prepared and screened as potential antiepileptics. Among the new derivatives tested, only 2b and 3c exhibited adequate anticonvulsant effects, whereas 3d and 4d were found to be convulsants per se.
Assuntos
Anticonvulsivantes/síntese química , Anticonvulsivantes/farmacologia , Tioureia/análogos & derivados , Ureia/análogos & derivados , Animais , Masculino , Camundongos , Relação Estrutura-Atividade , Tioureia/síntese química , Tioureia/farmacologia , Ureia/síntese química , Ureia/farmacologiaRESUMO
Starting from 3,6-dichloro-N-(2-chloro-5-nitrophenyl)-pyridazine-4-carboxamide (7) a series of 6,11-dialkylated pyridazino- [3,4-b][1,5]benzodiazepin-5-ones with a 3-chloro-8-nitro, 8-amino, 8-acetylamino, or 8-chloro substitution pattern was prepared via N-alkyl-3-alkylamino-6-chloro-N-(2-chloro-5-nitrophenyl) -pyridazine-4-carboxamides. The new compounds were screened as non-nucleoside reverse transcriptase inhibitors. The influence of the substitution pattern in compounds 10-13 on inhibitory potency is discussed.
Assuntos
Fármacos Anti-HIV/síntese química , Fármacos Anti-HIV/farmacologia , Benzodiazepinonas/síntese química , Benzodiazepinonas/farmacologia , Transcriptase Reversa do HIV/efeitos dos fármacos , Inibidores da Transcriptase Reversa/síntese química , Inibidores da Transcriptase Reversa/farmacologia , Transcriptase Reversa do HIV/metabolismoRESUMO
The synthesis of a variety of novel compounds structurally related to the antimicrobial natural product pyridazomycin via alkylation of appropriate azine and diazine derivatives is reported. Based on the results of preliminary antimicrobial tests the dependence of antimicrobial activity from several structural features of pyridazomycin is discussed.
Assuntos
Aminoácidos/síntese química , Anti-Infecciosos/síntese química , Antifúngicos/química , Antifúngicos/farmacologia , Aminoácidos/farmacologia , Anti-Infecciosos/farmacologia , Fenômenos Químicos , Físico-Química , Cromatografia Líquida de Alta Pressão , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Piridazinas/química , Piridazinas/farmacologiaRESUMO
Preparation of series of pyridine-, pyridazine-, and pyrazine-derived carboxamides bearing at the ring N-atom an alkyl side-chain with a terminal carboxylic group (7-11) or with a terminal acetylamino malonic ester moiety (13-17, 19-23) is described. Two desaza-pyridazomycin derivatives (24, 26) and homologs thereof (25, 27) were synthesised. The novel compounds which are structurally related to the antifungal antibiotic pyridazomycin were screened for antifungal activity: preliminary in vitro tests showed no activity.