TOXOPLASMA GONDII PREVALENCE, PARTIAL GENOTYPES, AND SPATIAL VARIATION IN NORTH AMERICAN RIVER OTTERS (LONTRA CANADENSIS) IN THE UPPER PENINSULA OF MICHIGAN, USA.

Toxoplasma gondii is a ubiquitous parasitic protozoan that poses a health threat to wildlife and human health worldwide. Oocysts shed into the environment in felid host feces may persist for several years. Runoff from rainfall and snowmelt may carry the oocysts into waterways. Semiaquatic mammals such as the Northern American river otter (Lontra canadensis) are particularly at risk of exposure, as they may encounter infective stages in both terrestrial and aquatic environments. Despite this risk, only a small number of studies have examined the prevalence of T. gondii in US river otter populations. Tongue tissue was sampled from 124 otters from the Upper Peninsula of Michigan submitted by trappers to the Michigan Department of Natural Resources in the 2018-19 harvest season. Following DNA extraction, a portion of the B1 T. gondii gene was amplified with PCR. A subset of positive samples was genotyped for comparison with known T. gondii sequences. Of the 124 tongue samples, 35 (28%) were positive for T. gondii. Prevalence did not differ significantly between sexes or age classes across the entire study area. Most (53.8%) of the genotyped samples were type 4 (type 12), a genotype commonly found in North American wildlife. Genotypes 127 and 197 were also found. Three clusters of T. gondii prevalence were identified through SaTScan analysis, although they were not significant. When modeling prevalence of T. gondii with covariates at individual otter locations, the top three models included the presence of Sarcocystis, area of exotic plants, area of agriculture, and sex of the otter. Our results suggest that T. gondii is widespread in otter populations in the Upper Peninsula of Michigan.

The Hydrologic Role of Urban Green Space in Mitigating Flooding (Luohe, China).

Even if urban catchments are adequately drained by sewer infrastructures, flooding hotspots develop where ongoing development and poor coordination among utilities conspire with land use and land cover, drainage, and rainfall. We combined spatially explicit land use/land cover data from Luohe City (central China) with soil hydrology (as measured, green space hydraulic conductivity), topography, and observed chronic flooding to analyze the relationships between spatial patterns in pervious surface and flooding. When compared to spatial-structural metrics of land use/cover where flooding was commonly observed, we found that some areas expected to remain dry (given soil and elevation characteristics) still experienced localized flooding, indicating hotspots with overwhelmed sewer infrastructure and a lack of pervious surfaces to effectively infiltrate and drain rainfall. Next, we used curve numbers to represent the composite hydrology of different land use/covers within both chronic flooding and dry (non-flooding) circles of 750 m diameter, and local design storms to determine the anticipated average proportion of runoff. We found that dry circles were more permeable (curve number (mean ± std. error) = 74 ± 2, n = 25) than wetter, flooded circles (curve number = 87 ± 1). Given design storm forcing (20, 50, 100 years' recurrence interval, and maximum anticipated storm depths), dry points would produce runoff of 26 to 35 percent rainfall, and wet points of 52 to 61 percent of applied rainfall. However, we estimate by simulation that runoff reduction benefits would decline once infiltration-excess (Hortonian) runoff mechanisms activate for storms with precipitation rates in excess of an average of 21 mm/h, contingent on antecedent moisture conditions. Our spatial metrics indicate that larger amounts and patches of dispersed green space mitigate flooding risk, while aggregating buildings (roofs) and green space into larger, separate areas exacerbates risk.

Hybridization, agency discretion, and implementation of the U.S. Endangered Species Act.

The U.S. Endangered Species Act (ESA) requires that the "best available scientific and commercial data" be used to protect imperiled species from extinction and preserve biodiversity. However, it does not provide specific guidance on how to apply this mandate. Scientific data can be uncertain and controversial, particularly regarding species delineation and hybridization issues. The U.S. Fish and Wildlife Service (FWS) had an evolving hybrid policy to guide protection decisions for individuals of hybrid origin. Currently, this policy is in limbo because it resulted in several controversial conservation decisions in the past. Biologists from FWS must interpret and apply the best available science to their recommendations and likely use considerable discretion in making recommendations for what species to list, how to define those species, and how to recover them. We used semistructured interviews to collect data on FWS biologists' use of discretion to make recommendations for listed species with hybridization issues. These biologists had a large amount of discretion to determine the best available science and how to interpret it but generally deferred to the scientific consensus on the taxonomic status of an organism. Respondents viewed hybridization primarily as a problem in the context of the ESA, although biologists who had experience with hybridization issues were more likely to describe it in more nuanced terms. Many interviewees expressed a desire to continue the current case-by-case approach for handling hybridization issues, but some wanted more guidance on procedures (i.e., a "flexible" hybrid policy). Field-level information can provide critical insight into which policies are working (or not working) and why. The FWS biologists' we interviewed had a high level of discretion, which greatly influenced ESA implementation, particularly in the context of hybridization.

Bioenergy and Biodiversity: Key Lessons from the Pan American Region.

Understanding how large-scale bioenergy production can affect biodiversity and ecosystems is important if society is to meet current and future sustainable development goals. A variety of bioenergy production systems have been established within different contexts throughout the Pan American region, with wide-ranging results in terms of documented and projected effects on biodiversity and ecosystems. The Pan American region is home to the majority of commercial bioenergy production and therefore the region offers a broad set of experiences and insights on both conflicts and opportunities for biodiversity and bioenergy. This paper synthesizes lessons learned focusing on experiences in Canada, the United States, and Brazil regarding the conflicts that can arise between bioenergy production and ecological conservation, and benefits that can be derived when bioenergy policies promote planning and more sustainable land-management systems. We propose a research agenda to address priority information gaps that are relevant to biodiversity concerns and related policy challenges in the Pan American region.

How much is enough? Minimal responses of water quality and stream biota to partial retrofit stormwater management in a suburban neighborhood.

Decentralized stormwater management approaches (e.g., biofiltration swales, pervious pavement, green roofs, rain gardens) that capture, detain, infiltrate, and filter runoff are now commonly used to minimize the impacts of stormwater runoff from impervious surfaces on aquatic ecosystems. However, there is little research on the effectiveness of retrofit, parcel-scale stormwater management practices for improving downstream aquatic ecosystem health. A reverse auction was used to encourage homeowners to mitigate stormwater on their property within the suburban, 1.8 km(2) Shepherd Creek catchment in Cincinnati, Ohio (USA). In 2007-2008, 165 rain barrels and 81 rain gardens were installed on 30% of the properties in four experimental (treatment) subcatchments, and two additional subcatchments were maintained as controls. At the base of the subcatchments, we sampled monthly baseflow water quality, and seasonal (5×/year) physical habitat, periphyton assemblages, and macroinvertebrate assemblages in the streams for the three years before and after treatment implementation. Given the minor reductions in directly connected impervious area from the rain barrel installations (11.6% to 10.4% in the most impaired subcatchment) and high total impervious levels (13.1% to 19.9% in experimental subcatchments), we expected minor or no responses of water quality and biota to stormwater management. There were trends of increased conductivity, iron, and sulfate for control sites, but no such contemporaneous trends for experimental sites. The minor effects of treatment on streamflow volume and water quality did not translate into changes in biotic health, and the few periphyton and macroinvertebrate responses could be explained by factors not associated with the treatment (e.g., vegetation clearing, drought conditions). Improvement of overall stream health is unlikely without additional treatment of major impervious surfaces (including roads, apartment buildings, and parking lots). Further research is needed to define the minimum effect threshold and restoration trajectories for retrofitting catchments to improve the health of stream ecosystems.

Strengths and weaknesses of common sustainability indices for multidimensional systems.

Sustainability is rapidly moving from an abstract concept to a measurable state of dynamic human-ecological systems. The large number of economic, social, and environmental indicators currently available provides an unwieldy view of system sustainability. To aid policy decisions, these indicators are therefore either presented in the context of a conceptual framework, or quantitatively aggregated into indices. Due to the quantitative nature of sustainability indices, their results may be given more weight by scientists and policy-makers. However, policy decisions can be ineffective or even counterproductive if they do not consider factors which influence index behavior: the scale of the available data and choice of system boundaries; the inclusion, transformation, and weighting of indicator data; and the aggregation method used. As previous reviews have demonstrated, sustainability indices do not rank countries consistently, even when using some of the same indicator data. Several improvements would increase the utility of sustainability indices for policy decisions, particularly the identification of biases introduced by the index methodology and data inclusion. The discrepancy of current sustainability indices due to theoretical or methodological differences supports the use of several complementary indices.