Enzymatic pretreatment for cellulose nanofiber production: Understanding morphological changes and predicting reducing sugar concentration.

Enzymatic pretreatment plays a crucial role in producing cellulose nanofibers (CNFs) before fibrillation. While previous studies have explored how treatment severity affects CNF characteristics, there remains a lack of suitable parameters to monitor real-time enzymatic processes and fully comprehend the link between enzymatic action, fibrillation, and CNF properties. This study focuses on evaluating the impact of enzyme charge (using a monocomponent endoglucanase) and treatment time on cellulose fiber morphology and reducing sugar generation. For the first time, a random forest (RF) model is developed to predict reducing sugar concentration based on easily measurable process conditions (e.g., stirrer power consumption) and fiber/suspension characteristics like fines content and apparent viscosity. Polarized light optical microscopy was found to be a suitable technique to evaluate the morphological changes that fibers experience during enzymatic pretreatment. The research also revealed that endoglucanases initially induce surface fibrillation, releasing fine fibers into the suspension, followed by fiber swelling and shortening. Furthermore, the effect of enzymatic pretreatment on resulting CNF characteristics was studied at two fibrillation intensities, indicating that a high enzyme charge and short treatment times (e.g., 90 min) are sufficient to produce CNFs with a nanofibrillation yield of 19-23 % and a cationic demand ranging from 220 to 275 µeq/g. This work introduces a well-modeled enzymatic pretreatment process, unlocking its potential and reducing uncertainties for future upscaling endeavors.

Durable Nanocellulose-Stabilized Emulsions of Dithizone/Chloroform in Water for Hg2+ Detection: A Novel Approach for a Classical Problem.

The use of dithizone (DTZ) for colorimetric heavy-metal detection is approximately one century old. However, its pending stability issues and the need for simple indicators justify further research. Using cellulose nanofibers, we attained DTZ-containing emulsions with high stability. These emulsions had water (at least 95 wt %) and acetic acid (1-8 mL/L) conforming the continuous phase, while dispersed droplets of diameter <1 µm contained chloroform-solvated DTZ (3 wt %). The solvation cluster was computed by molecular dynamics simulations, suggesting that chloroform slightly reduces the dihedral angle between the two sides of the thiocarbazone chain. Nanocellulose concentrations over 0.2 wt % sufficed to obtain macroscopically homogeneous mixtures with no phase separation. Furthermore, the rate of degradation of DTZ in the nanocellulose-stabilized emulsion did not differ significantly from a DTZ/chloroform solution, outperforming DTZ/toluene and DTZ/acetonitrile. Not only is the emulsion readily and immediately responsive to mercury(II), but it also decreases interferences from other ions and from natural samples. Unexpectedly, neither lead(II) nor cadmium(II) triggered a visual response at trace concentrations. The limit of detection of these emulsions is 15 µM or 3 mg/L, exceeding WHO limits for mercury(II) in drinking water, but they could be effective at raising alarms.

Improving the Barrier Properties of Paper to Moisture, Air, and Grease with Nanocellulose-Based Coating Suspensions.

Food packaging manufacturers often resort to lamination, typically with materials which are neither non-biodegradable nor biobased polymers, to confer barrier properties to paper and cardboard. The present work considers a greener solution: enhancing paper's resistance to moisture, grease, and air by aqueous coating suspensions. For hydrophobization, a combined approach between nanocellulose and common esterifying agents was considered, but the water vapor transmission rate (WVTR) remained excessively high for the goal of wrapping moisture-sensitive products (>600 g m−2 d−1). Nonetheless, oil-repellant surfaces were effectively obtained with nanocellulose, illite, sodium alginate, and/or poly(vinyl alcohol) (PVA), reaching Kit ratings up to 11. Regarding air resistance, mineral-rich coatings attained values above 1000 Gurley s. In light of these results, nanocellulose, minerals, PVA, pullulan, alginate, and a non-ionic surfactant were combined for multi-purpose coating formulations. It is hypothesized that these materials decrease porosity while complementing each other's flaws, e.g., PVA succeeds at decreasing porosity but has low dimensional stability. As an example, a suspension mostly constituted by nanocellulose, sizing agents, minerals and PVA yielded a WVTR of roughly 100 g m−2 d−1, a Kit rating of 12, and an air resistance above 300 s/100 mL. This indicates that multi-purpose coatings can be satisfactorily incorporated into paper structures for food packaging applications, although not as the food contact layer.