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1.
Opt Express ; 31(6): 10775-10793, 2023 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-37157617

RESUMO

We present an empirical measurement of the dark count rate seen in a large-format MKID array identical to those currently in use at observatories such as Subaru on Maunakea. This work provides compelling evidence for their utility in future experiments that require low-count rate, quiet environments such as dark matter direct detection. Across the bandpass from 0.946-1.534 eV (1310-808 nm) an average count rate of (1.847 ± 0.003) × 10-3 photons/pixel/s is measured. Breaking this bandpass into 5 equal-energy bins based on the resolving power of the detectors we find the average dark count rate seen in an MKID is (6.26 ± 0.04) × 10-4 photons/pixel/s from 0.946-1.063 eV and (2.73 ± 0.02) × 10-4 photons/pixel/s at 1.416-1.534eV. Using lower-noise readout electronics to read out a single MKID pixel we demonstrate that the events measured while the detector is not illuminated largely appear to be a combination of real photons, possible fluorescence caused by cosmic rays, and phonon events in the array substrate. We also find that using lower-noise readout electronics on a single MKID pixel we measure a dark count rate of (9.3 ± 0.9) × 10-4 photons/pixel/s over the same bandpass (0.946-1.534 eV) With the single-pixel readout we also characterize the events when the detectors are not illuminated and show that these responses in the MKID are distinct from photons from known light sources such as a laser, likely coming from cosmic ray excitations.

2.
Phys Rev Lett ; 129(1): 017701, 2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35841577

RESUMO

Microwave kinetic inductance detectors (MKIDs) sensitive to light in the ultraviolet to near-infrared wavelengths are superconducting microresonators that are capable of measuring photon arrival times to microsecond precision and estimating each photon's energy. The resolving power of nonmembrane MKIDs has remained stubbornly around 10 at 1 µm despite significant improvements in the system noise. Here we show that the resolving power can be roughly doubled with a simple bilayer design without needing to place the device on a membrane, avoiding a significant increase in fabrication complexity. Based on modeling of the phonon propagation, we find that the majority of the improvement comes from the inability of high energy phonons to enter the additional layer due to the lack of available phonon states.

3.
Rev Sci Instrum ; 91(12): 124705, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33379935

RESUMO

We present the development of a second generation digital readout system for photon counting microwave kinetic inductance detector (MKID) arrays operating in the optical and near-infrared wavelength bands. Our system retains much of the core signal processing architecture from the first generation system but with a significantly higher bandwidth, enabling the readout of kilopixel MKID arrays. Each set of readout boards is capable of reading out 1024 MKID pixels multiplexed over 2 GHz of bandwidth; two such units can be placed in parallel to read out a full 2048 pixel microwave feedline over a 4 GHz-8 GHz band. As in the first generation readout, our system is capable of identifying, analyzing, and recording photon detection events in real time with a time resolution of order a few microseconds. Here, we describe the hardware and firmware, and present an analysis of the noise properties of the system. We also present a novel algorithm for efficiently suppressing IQ mixer sidebands to below -30 dBc.

4.
BMC Plant Biol ; 19(1): 202, 2019 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-31096905

RESUMO

BACKGROUND: The Fertilization-related kinases (FRK) form a class that belongs to the MEKK subfamily of plant MAPKKKs. It was recently shown that FRK class kinases expanded during angiosperm evolution, reaching their maximum numbers in the lineage leading to solanaceous species and culminating in the Solanum genus where they account for more than 40% of the total MEKKs. The first members studied, ScFRK1 and ScFRK2 were shown to play a pivotal role in gametophyte development in the wild potato species Solanum chacoense. RESULTS: ScFRK3 is also involved in gametophyte development. ScFRK3 is expressed in developing pollen and young ovules, reaching its highest level immediately after meiosis and during the mitosis steps in both gametophytes. Hence, three independent lines of ScFRK3 RNAi mutant plants showed decreased number of seeds per fruit. We also observed an important number of degenerated embryo sac in mature ovary. Analysis of ovule development showed that most embryo sac did not enter mitosis I in ScFRK3 RNAi mutant plants. Severe lethality was also observed during male gametophyte development, pollen being arrested before mitosis I, as observed in the female gametophyte. Obvious defects in vegetative organs were not observed, emphasizing the reproductive roles of the FRK class kinases. To isolate MAP kinases acting downstream of ScFRK3, a de novo S. chacoense transcriptome from male and female reproductive organs was assembled. Of the five ScMKKs and 16 ScMPKs retrieved, only the ScMKK3 interacted with ScFRK3, while only the ScMPK13 interacted with ScMKK3, leading to an apparent single three-tiered canonical MAP kinase cascade combination involving ScFRK3-ScMKK3-ScMPK13. CONCLUSIONS: The ScFRK3 MAPKKK is involved in a signaling cascade that regulates both male and female gamete development, and most probably act upstream of ScMKK3 and ScMPK13.


Assuntos
Óvulo Vegetal/crescimento & desenvolvimento , Proteínas de Plantas/metabolismo , Pólen/crescimento & desenvolvimento , Proteínas Quinases/metabolismo , Solanum/crescimento & desenvolvimento , Hibridização In Situ , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia , Proteínas Quinases/genética , Proteínas Quinases/fisiologia , RNA de Plantas/metabolismo , Solanum/enzimologia , Solanum/genética , Técnicas do Sistema de Duplo-Híbrido
5.
Plant Signal Behav ; 14(8): 1620059, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31131700

RESUMO

Fertilization-related kinase (FRK) is a group of the mitogen-activated protein kinase kinase kinase (MAP3K or MEKK) that has proliferated in Solanaceae species. Studies on the wild potato Solanum chacoense have shown that three ScFRKs are directly involved in female gametophyte development. Decreasing the expression of ScFRK1 and ScFRK3 by RNA interference lead to embryonic sac development arrest at the functional megaspore (FM) stage. As for ScFRK2, the first FRK studied, antisense and co-suppression lines showed no abnormality, while overexpression lines lead to a drastic decrease in seed numbers, presumably caused by a conversion of the ovule into a carpel-like structure. Here we show that in ScFRK2 overexpression lines, carpel-like structures from the ovule cannot explain the drastic decrease in seeds considering the low percentage of these carpel-like structures but occurs in early ovule development as observed in Scfrk1 and Scfrk3 knockdown mutants were most ovules are arrested at the FM stage. The highly similar phenotype from knockdown mutants (Scfrk1 and Scfrk3) and ScFRK2 overexpression lines suggests that these MAP kinases could operate antagonistically through a balance between ScFRK1 and 3 on one side and ScFRK2 on the other. This study strongly suggests the importance of the FRK family expression levels during early stages of ovule development in Solanum chacoense embryo sac.


Assuntos
MAP Quinase Quinase Quinases/metabolismo , Plantas Geneticamente Modificadas/embriologia , Plantas Geneticamente Modificadas/enzimologia , Solanum/embriologia , Solanum/enzimologia , Regulação da Expressão Gênica de Plantas , MAP Quinase Quinase Quinases/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Sementes/embriologia , Sementes/enzimologia , Sementes/metabolismo , Solanum/metabolismo
6.
Rev Sci Instrum ; 83(4): 044702, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22559560

RESUMO

Microwave kinetic inductance detectors (MKIDs) are superconducting detectors capable of counting single photons and measuring their energy in the UV, optical, and near-IR. MKIDs feature intrinsic frequency domain multiplexing (FDM) at microwave frequencies, allowing the construction and readout of large arrays. Due to the microwave FDM, MKIDs do not require the complex cryogenic multiplexing electronics used for similar detectors, such as transition edge sensors, but instead transfer this complexity to room temperature electronics where they present a formidable signal processing challenge. In this paper, we describe the first successful effort to build a readout for a photon counting optical/near-IR astronomical instrument, the ARray Camera for Optical to Near-infrared Spectrophotometry. This readout is based on open source hardware developed by the Collaboration for Astronomy Signal Processing and Electronics Research. Designed principally for radio telescope backends, it is flexible enough to be used for a variety of signal processing applications.

7.
Opt Express ; 20(2): 1503-11, 2012 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-22274494

RESUMO

Microwave Kinetic Inductance Detectors, or MKIDs, have proven to be a powerful cryogenic detector technology due to their sensitivity and the ease with which they can be multiplexed into large arrays. A MKID is an energy sensor based on a photon-variable superconducting inductance in a lithographed microresonator, and is capable of functioning as a photon detector across the electromagnetic spectrum as well as a particle detector. Here we describe the first successful effort to create a photon-counting, energy-resolving ultraviolet, optical, and near infrared MKID focal plane array. These new Optical Lumped Element (OLE) MKID arrays have significant advantages over semiconductor detectors like charge coupled devices (CCDs). They can count individual photons with essentially no false counts and determine the energy and arrival time of every photon with good quantum efficiency. Their physical pixel size and maximum count rate is well matched with large telescopes. These capabilities enable powerful new astrophysical instruments usable from the ground and space. MKIDs could eventually supplant semiconductor detectors for most astronomical instrumentation, and will be useful for other disciplines such as quantum optics and biological imaging.


Assuntos
Fenômenos Astronômicos , Raios Infravermelhos , Óptica e Fotônica/métodos , Telescópios , Raios Ultravioleta , Condutividade Elétrica , Desenho de Equipamento , Espectroscopia de Luz Próxima ao Infravermelho/métodos
8.
Nature ; 425(6960): 817-21, 2003 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-14574407

RESUMO

Cryogenic detectors are extremely sensitive and have a wide variety of applications (particularly in astronomy), but are difficult to integrate into large arrays like a modern CCD (charge-coupled device) camera. As current detectors of the cosmic microwave background (CMB) already have sensitivities comparable to the noise arising from the random arrival of CMB photons, the further gains in sensitivity needed to probe the very early Universe will have to arise from large arrays. A similar situation is encountered at other wavelengths. Single-pixel X-ray detectors now have a resolving power of DeltaE < 5 eV for single 6-keV photons, and future X-ray astronomy missions anticipate the need for 1,000-pixel arrays. Here we report the demonstration of a superconducting detector that is easily fabricated and can readily be incorporated into such an array. Its sensitivity is already within an order of magnitude of that needed for CMB observations, and its energy resolution is similarly close to the targets required for future X-ray astronomy missions.

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