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Sci Rep ; 8(1): 13575, 2018 09 11.
Artigo em Inglês | MEDLINE | ID: mdl-30206304

RESUMO

Catechins, including catechin (C) and epicatechin (E), are the main type of flavonoids in cacao seeds. They play important roles in plant defense and have been associated with human health benefits. Although flavonoid biosynthesis has been extensively studied using in vitro and in vivo models, the regulatory mechanisms controlling their accumulation under light/dark conditions remain poorly understood. To identify differences in flavonoid biosynthesis (particularly catechins) under different light treatments, we used cacao cell suspensions exposed to white-blue light and darkness during 14 days. RNA-Seq was applied to evaluate differential gene expression. Our results indicate that light can effectively regulate flavonoid profiles, inducing a faster accumulation of phenolic compounds and shifting E/C ratios, in particular as a response to switching from white to blue light. The results demonstrated that HY5, MYB12, ANR and LAR were differentially regulated under light/dark conditions and could be targeted by overexpression aiming to improve catechin synthesis in cell cultures. In conclusion, our RNA-Seq analysis of cacao cells cultured under different light conditions provides a platform to dissect key aspects into the genetic regulatory network of flavonoids. These light-responsive candidate genes can be used further to modulate the flavonoid production in in vitro systems with value-added characteristics.


Assuntos
Cacau/genética , Catequina/biossíntese , Flavonoides/biossíntese , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Sementes/genética , Fatores de Transcrição/genética , Cacau/citologia , Cacau/metabolismo , Cacau/efeitos da radiação , Catequina/genética , Flavonoides/genética , Redes Reguladoras de Genes , Luz , Fotoperíodo , Células Vegetais/metabolismo , Células Vegetais/efeitos da radiação , Proteínas de Plantas/classificação , Proteínas de Plantas/metabolismo , Sementes/citologia , Sementes/metabolismo , Sementes/efeitos da radiação , Análise de Sequência de RNA , Fatores de Transcrição/classificação , Fatores de Transcrição/metabolismo , Transcriptoma
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