Sediment DNA metabarcoding and morphology provide complementary insight into macrofauna and meiobenthos response to environmental gradients in an Arctic glacial fjord.

Arctic fjords ecosystems are highly dynamic, with organisms exposed to various natural stressors along with productivity clines driven by advection of water masses from shelves. The benthic response to these environmental clines has been extensively studied using traditional, morphology-based approaches mostly focusing on macroinvertebrates. In this study we analyse the effects of glacially mediated disturbance on the biodiversity of benthic macrofauna and meiobenthos (meiofauna and Foraminifera) in a Svalbard fjord by comparing morphology and eDNA metabarcoding. Three genetic markers targeting metazoans (COI), meiofauna (18S V1V2) and Foraminifera (18S 37f) were analyzed. Univariate measures of alpha diversity and multivariate compositional dissimilarities were calculated and tested for similarities in response to environmental gradients using correlation analysis. Our study showed different taxonomic composition of morphological and molecular datasets for both macrofauna and meiobenthos. Some taxonomic groups while abundant in metabarcoding data were almost absent in morphology-based inventory and vice versa. In general, species richness and diversity measures in macrofauna morphological data were higher than in metabarcoding, and similar for the meiofauna. Both methodological approaches showed different patterns of response to the glacially mediated disturbance for the macrofauna and the meiobenthos. Macrofauna showed an evident distinction in taxonomic composition and a dramatic cline in alpha diversity indices between the outer and inner parts of fjord, while the meiobenthos showed a gradual change and more subtle responses to environmental changes along the fjord axis. The two methods can be seen as complementing rather than replacing each other. Morphological approach provides more accurate inventory of larger size species and more reliable quantitative data, while metabarcoding allows identification of inconspicuous taxa that are overlooked in morphology-based studies. As different taxa may show different sensitivities to environmental changes, both methods shall be used to monitor marine biodiversity in Arctic ecosystems and its response to dramatically changing environmental conditions.

Isolation and identification of microplastics in infant formulas - A potential health risk for children.

Microplastics (MPs) are plastic particles between 0.1 and 5,000 µm in size that can contaminate food. Unfortunately, to date, little attention has been paid to analyzing the presence of such particles in baby foods. The present study aimed to determine the degree of contamination of infant formula with MPs. A total of thirty products were subjected to analysis. The research methodology used included the isolation of plastic particles, identification and characterization of MPs using advanced microscopic and spectroscopic techniques. Microplastics were detected in all tested samples. The most frequently identified polymers were polyamide, polyethylene, polypropylene, and poly(ethylene terephthalate). The particles exhibited diverse forms, including fibers, fragments, and films, displaying a range of colors such as colorless, black, and brown particles. Furthermore, the daily intake of MPs by children fed exclusively infant formula was estimated to be approximately 49 ± 32 MPs. This poses a potential health risk for the youngest.

Airborne and marine microplastics from an oceanographic survey at the Baltic Sea: An emerging role of air-sea interaction?

Microplastics (MPs) pollution is one of the most important problems of the Earth. They have been found in all the natural environments, including oceans and the atmosphere. In this study, the concentrations of both atmospheric and marine MPs were measured over the Baltic along a research cruise that started in the Gdansk harbour, till the Gotland island, and the way back. A deposition box (based on a combination of active/passive sampling) was used to collect airborne MPs while, marine MPs concentrations were investigated during the cruise using a dedicated net. Ancillary data were obtained using a combination of particle counters (OPC, LAS and CPC), Aethalometer (AE33 Magee Scientific), spectrofluorometer (sea surface samples, Varian Cary Eclipse), and meteorological sensors. Results showed airborne microplastics average concentrations higher in the Gdansk harbour (161 ± 75 m-3) compared to the open Baltic Sea and to the Gotland island (24 ± 9 and 45 ± 20 m-3). These latter values are closer to the ones measured in the sea (79 ± 18 m-3). The MPs composition was investigated using µ-Raman (for the airborne ones) and FTIR (for marine ones); similar results (e.g. polyethylene, polyethylene terephthalates, polyurethane) were found in the two environmental compartments. The concentrations and similar composition in air and sea suggested a linkage between the two compartments. For this purpose, the atmospheric MPs' equivalent aerodynamic diameter was calculated (28 ± 3 µm) first showing the capability of atmospheric MPs to remain suspended in the air. At the same time, the computed turnover times (0.3-90 h; depending on MPs size) limited the transport distance range. The estimated MPs sea emission fluxes (4-18 ∗ 106 µm3 m-2 s-1 range) finally showed the contemporary presence of atmospheric transport together with a continuous emission from the sea surface enabling a grasshopper long-range transport of microplastics across the sea.

PCBs, HCB and PAHs in the seawater of Arctic fjords - Distribution, sources and risk assessment.

In the present study, we examine contamination with PCBs, HCB and PAHs in the seawater of Arctic fjords (Hornsund, Kongsfjorden and Adventfjorden) which differ in environmental conditions and are particularly sensitive to climate change. We also investigate how the melting glaciers and ocean currents may affect the distribution and fate of target compounds in the seawater column in the fjords. The ∑7 PCB, HCB and ∑12 PAH concentrations in seawater ranged from, respectively: 0.002 to 41.2 ng/L; from LOQ to 233 ng/L; and from 0.196 to 311 ng/L. The research indicates that the concentrations of contaminants detected in Arctic fjords depend on the physicochemical properties of these compounds, local human activity and occurrence of glacier meltwaters. Detected HCB and PAH concentrations in most of the seawater samples were at levels classified as harmless, however in 30 out of 80 analysed suspended particulate matter samples some compounds were present at toxic levels.

Latitudinal consistency of biomass size spectra - benthic resilience despite environmental, taxonomic and functional trait variability.

Global warming is expected to cause reductions in organism body size, a fundamental biological unit important in determining biological processes. Possible effects of increasing temperature on biomass size spectra in coastal benthic communities were investigated. We hypothesized higher proportions of smaller size classes in warmer conditions. Soft bottom infauna samples were collected in six Norwegian and Svalbard fjords, spanning wide latitudinal (60-81°N) and bottom water temperature gradients (from -2 to 8 °C). Investigated fjords differed in terms of environmental settings (e.g., pigments or organic carbon in sediments). The slopes of normalised biomass size spectra (NBSS) did not differ among the fjords, while the benthic biomass and NBSS intercepts varied and were related to chlorophyll a and δ13C in sediments. The size spectra based on both abundance and biomass remained consistent, regardless of the strong variability in macrofauna taxonomic and functional trait composition. Variable relationships between temperature and body size were noted for particular taxa. Our results indicate that while benthic biomass depends on the nutritional quality of organic matter, its partitioning among size classes is consistent and independent of environmental and biological variability. The observed size structure remains a persistent feature of studied communities and may be resilient to major climatic changes.