RESUMO
Treatment of human embryonic cell line CLV 102 with N-methyl-N'-nitro-N-nitrosoguanidine or bleomycin was used to induce DNA repair synthesis. Both the repair synthesis induced by these agents and reactivation of UV-damaged pseudorabies virus were inhibited by aphidicolin.
Assuntos
Afidicolina/farmacologia , Bleomicina/farmacologia , Reparo do DNA/efeitos dos fármacos , DNA Polimerase Dirigida por DNA/fisiologia , Metilnitronitrosoguanidina/farmacologia , Ativação Viral/efeitos dos fármacos , Linhagem Celular , DNA Polimerase Dirigida por DNA/efeitos dos fármacos , Herpesvirus Suídeo 1/crescimento & desenvolvimento , Herpesvirus Suídeo 1/efeitos da radiação , Humanos , Raios UltravioletaRESUMO
Mouse embryo cells nonproductively infected with human cytomegalovirus differed from noninfected cells by the impaired ability to grow in the medium containing homocysteine instead of methionine. Virus infection of mouse embryo cells grown in both kinds of media resulted in the increase of protein synthesis. In the infected cells grown on homocysteine this increase was followed by a quick decrease. The effects of homocysteine substitution could be abolished by the addition of low amounts of methionine (0.1 mM). Methionine uptake in the infected cells grown on homocysteine for 48 h was significantly higher than that in the noninfected cells.
