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1.
Environ Pollut ; 235: 1006-1014, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29751396

RESUMO

The problem of effective assessment of risk posed by complex mixtures of toxic chemicals in the environment is a major challenge for government regulators and industry. The biological effect of the individual contaminants, where these are known, can be measured; but the problem lies in relating toxicity to the multiple constituents of contaminant cocktails. The objective of this study was to test the hypothesis that diverse contaminant mixtures may cause a greater toxicity than the sum of their individual parts, due to synergistic interactions between contaminants with different intracellular targets. Lysosomal membrane stability in hemocytes from marine mussels was used for in vitro toxicity tests; and was coupled with analysis using the isobole method and a linear additive statistical model. The findings from both methods have shown significant emergent synergistic interactions between environmentally relevant chemicals (i.e., polycyclic aromatic hydrocarbons, pesticides, biocides and a surfactant) when exposed to isolated hemocytes as a mixture of 3 & 7 constituents. The results support the complexity-based hypothesis that emergent toxicity occurs with increasing contaminant diversity, and raises questions about the validity of estimating toxicity of contaminant mixtures based on the additive toxicity of single components. Further experimentation is required to investigate the potential for interactive effects in mixtures with more constituents (e.g., 50-100) at more environmentally realistic concentrations in order to test other regions of the model, namely, very low concentrations and high diversity. Estimated toxicant diversity coupled with tests for lysosomal damage may provide a potential tool for determining the toxicity of estuarine sediments, dredge spoil or contaminated soil.


Assuntos
Bivalves/fisiologia , Poluentes Ambientais/toxicidade , Hemócitos/efeitos dos fármacos , Testes de Toxicidade/métodos , Animais , Hemócitos/fisiologia , Compostos Orgânicos/toxicidade , Praguicidas , Hidrocarbonetos Policíclicos Aromáticos/toxicidade
2.
Mar Environ Res ; 54(3-5): 627-31, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12408628

RESUMO

There is a growing body of evidence to suggest that certain polycyclic aromatic hydrocarbons (PAHs) pose a greater hazard to aquatic organisms than previously demonstrated, due to their potential to cause photo-induced toxicity when exposed to ultraviolet (UV) radiation. The consequences of photo-induced toxicity are reported here for embryo-larval stages of the pacific oyster Crassostrea gigas, following exposure to pyrene and benzo[a]pyrene. During laboratory investigations, significant increases in toxicity were observed in the presence of environmentally attainable levels of UV-radiation, compared with embryos exposed to PAH alone, at levels previously deemed to have little acute biological effect. The phototoxicity of pyrene and benzo[a]pyrene completely inhibited the development to the D-shell larval stage when embryos were simultaneously exposed to 5 microg l(-1) PAH and ultraviolet light (UVB = 6.3 +/- 0.1 microW/cm2 and UVA = 456.2 +/- 55 microW/cm2). A linear relationship was also demonstrated for benzo[a]pyrene phototoxicity with decreasing UV light intensity.


Assuntos
Benzo(a)pireno/toxicidade , Corantes Fluorescentes/toxicidade , Mutagênicos/toxicidade , Ostreidae/embriologia , Ostreidae/crescimento & desenvolvimento , Pirenos/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Embrião não Mamífero/efeitos dos fármacos , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Dose Letal Mediana , Fotoquímica , Raios Ultravioleta/efeitos adversos
3.
Mar Environ Res ; 50(1-5): 273-7, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-11460703

RESUMO

Larval stages of two economically important flatfish, the sole (Solea solea) and turbot (Scophthalmus maximus) were exposed to ambient and elevated levels of UV-B. Sole larvae, which naturally occur in the plankton in early spring, demonstrated skin lesions at elevated levels of UV-B. Histopathology of the sole revealed cellular changes in the integument, characteristic of sunburn damage, with a reduction in the size of mucus-secreting cells and an increased epidermal thickening, especially at the highest doses of UV-B (2.15 KJ bio eff/m2). Pigmentation in the sole is restricted to a few isolated melanocytes. The integrity of the heavily pigmented skin of turbot appeared to be unaffected by comparable doses of UV-B. Both species have protective mechanisms, which minimize the effects of naturally-occurring levels of UV-B. However, sole appear to be poorly adapted to accommodate any further increase in solar radiation.


Assuntos
Linguados/embriologia , Pele/efeitos da radiação , Raios Ultravioleta , Adaptação Fisiológica , Animais , Larva/efeitos da radiação , Pele/patologia
4.
Mar Environ Res ; 50(1-5): 319-23, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-11460711

RESUMO

Cells exhibiting the multidrug resistance (MDR) phenotype demonstrate a decreased intracellular drug accumulation due to an active outward transport and decreased intracellular flux. This study demonstrates the inhibition of MDR in mussel (Mytilus edulis) embryos and larvae based on a simple bioassay. The development of embryos was assessed and abnormalities identified at key stages of development, including gastrulation, trochophore and prodissoconch stages. The incidence of developmental abnormalities was significantly increased in the presence of vinblastine, MMS, chloroquine, mitomycin-C, cadmium chloride and colchicine, compared to clean seawater. Consistently, there was a further increase in the number and severity of deformities observed when each toxin was added in the presence of verapamil. Larval growth was also significantly impaired in the presence of verapamil. Increased accumulation of fluorescent MDR dyes, such as rhodamine B, has been measured and shown to be verapamil sensitive. This bioassay encompasses a period of intense cellular activity during which the impairment of a number of critical processes results in abnormal growth and development.


Assuntos
Bivalves/efeitos dos fármacos , Bivalves/embriologia , Poluentes Químicos da Água/toxicidade , Animais , Bioensaio/métodos , Bioensaio/veterinária , Cloreto de Cádmio/toxicidade , Cloroquina/toxicidade , Colchicina/toxicidade , Resistência a Múltiplos Medicamentos , Sinergismo Farmacológico , Larva/efeitos dos fármacos , Metanossulfonato de Metila/toxicidade , Mitomicina/toxicidade , Fenótipo , Água do Mar , Verapamil/toxicidade , Vimblastina/toxicidade
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