RESUMO
The mdm2 oncogene is amplified and overexpressed in a variety of human tumours and the oncogenic potential of MDM2 is partly due to its ability to inactivate tumour suppressor p53 function. In the present communication we describe the cloning, sequence analysis and expression of the complete wildtype canine and equine mdm2 cDNAs. The encoded full-length canine and equine cDNAs show strong sequence homology with MDM2 proteins from other species and both cDNAs generate recombinant proteins of approximately 90 kDa. These data will allow for the role of this oncogene to be established in companion animal oncology.
Assuntos
Proteínas Nucleares , Proteínas Proto-Oncogênicas/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , DNA Complementar , Cães , Cavalos , Camundongos , Dados de Sequência Molecular , Proto-Oncogene Mas , Proteínas Proto-Oncogênicas/metabolismo , Proteínas Proto-Oncogênicas c-mdm2 , Proteínas Recombinantes/biossíntese , Análise de Sequência de DNA , Homologia de Sequência de AminoácidosRESUMO
An evolutionary conserved region of the canine tumour suppressor gene, p53, was PCR amplified and its DNA sequence determined. The 1003 bp fragment consisted of exons 5 to 8 and the intervening introns. A high level of sequence homology was demonstrated with human sequences, with the evolutionary conserved domains II, III, IV and V being identical.
Assuntos
Genes p53 , Sequência de Aminoácidos , Animais , Sequência de Bases , Sequência Conservada , Cães , Éxons , Humanos , Dados de Sequência Molecular , Análise de Sequência de DNA , Homologia de Sequência do Ácido NucleicoRESUMO
Papillomaviral DNA has been identified in peripheral blood cells of both cattle and humans with and without associated disease and it has been suggested that such cells may act as sites of viral latency. In order to investigate the possibility of latent papillomaviral infection in the aetiopathogenesis of the equine sarcoid, peripheral blood derived DNA samples from 20 healthy and 34 sarcoid-affected donkeys were subject to polymerase chain reaction (PCR) using papillomaviral specific primers. Analysis of blood derived DNA samples failed to demonstrate the presence of papillomaviral DNA in any animal. Screening of 37 matched sarcoid derived DNA samples confirmed the presence of BPV in 34 diseased donkeys. This study supports the hypothesis of BPV as an aetiological agent in the equine sarcoid and suggests that latent virus in circulating peripheral blood cells does not play a role in the pathogenesis and epidemiology of the equine sarcoid.
