RESUMO
Amoebic gill disease in teleost fish is caused by the marine parasite Neoparamoeba perurans. To date, the role of antimicrobial peptides ß-defensins and cathelicidins in this infection have not been explored. Using a high-throughput microfluidics quantitative polymerase chain reaction system (Biomark HD™ by Fluidigm), this study aimed to: firstly, to investigate organ-specific expression of antimicrobial peptide genes ß-defensin-1, -3 and -4 and cathelicidin 2 in healthy Atlantic salmon; secondly, to compare the expression of these antimicrobial peptide genes in healthy versus asymptomatic Atlantic salmon seven days post-challenge with Neoparamoeba perurans. Results from this study indicate expression of the ß-defensin and cathelicidin genes in the selected organs from healthy Atlantic salmon. Furthermore, a statistically significant upregulation of ß-defensins -3 and -4 and cathelicidin 2 was detected in gill of parasite-challenged salmon. The upregulated cathelicidin and ß-defensin genes in gill could indicate novel potential roles in innate immune responses to Neoparamoeba perurans.
Assuntos
Amebíase , Doenças dos Peixes , Salmo salar , Animais , Peptídeos Antimicrobianos , Expressão Gênica , Brânquias , Salmo salar/genéticaRESUMO
Of 150 Escherichia coli strains we cultured from specimens taken from cattle in Europe, 3 had elevated MICs against colistin. We assessed all 3 strains for the presence of the plasmid-mediated mcr-1 gene and identified 1 isolate as mcr-1-positive and co-resistant to ß-lactam, florfenicol, and fluoroquinolone antimicrobial compounds.
