Isolation of bacterial skin flora of healthy sheep, with comparison between frequent and minimal human handling.

BACKGROUND: Few data are available regarding skin bacterial flora of healthy sheep and meticillin-resistant Staphylococcus carriage. HYPOTHESIS/OBJECTIVES: To compare skin, ear and mucosal bacterial populations between minimally and frequently handled sheep; to determine whether the frequency of meticillin-resistant Staphylococcus aureus varied between groups. ANIMALS: One hundred and three healthy feedlot and show sheep from eight farms. METHODS: Swabs were collected from the dorsum, right ear and right nostril of each sheep. Two groups from each farm were evaluated, except from one farm, which had only one group. Bacterial isolates were identified to the genus or species level using phenotypic analysis or matrix-associated laser desorption/ionization time-of-flight mass spectrometry. Antimicrobial susceptibility testing and spa typing were performed on isolates of S. aureus. RESULTS: Sixteen bacterial genera were identified and 11 staphylococcal species, including S. aureus. The skin and mucosal bacterial flora were compared between the groups. The only statistically significant difference in bacteria was Streptococcus spp. on the dorsum (P = 0.0088), with carriage being more common in frequently handled sheep. Antimicrobial susceptibility testing did not find meticillin-resistant S. aureus. There was no significant difference in S. aureus carriage in the ear (P = 0.33), nostril (P = 0.43) or dorsum (P = 0.053) between frequently and minimally handled sheep. The S. aureus isolates belonged to six different spa types. Three were of the ST398 lineage. CONCLUSIONS AND CLINICAL IMPORTANCE: Sheep are a potential source of livestock-associated meticillin-sensitive Staphylococcus aureus ST398.

Adverse events in 50 cats with allergic dermatitis receiving ciclosporin.

Ciclosporin is an immunosuppressive drug that has been used to treat allergies and other immune-mediated diseases in cats, dogs and humans. Information about the adverse effects of ciclosporin in cats has been limited to smaller studies and case reports. Adverse effects in dogs are mainly gastrointestinal in nature, but humans can also experience hypertension and altered renal function. The aim of this retrospective case series study was to document the occurrence and clinical appearance of adverse events in cats receiving ciclosporin to treat allergic skin disease. The medical records of 50 cats with allergic dermatitis treated with oral ciclosporin (1.9-7.3 mg/kg/day) were reviewed. Adverse events occurred in 66% (33 cats). Adverse events likely to be associated with ciclosporin included the following: vomiting or diarrhoea within 1-8 weeks of receiving ciclosporin (24%), weight loss (16%), anorexia and subsequent hepatic lipidosis (2%) and gingival hyperplasia (2%). Other adverse events less likely to be associated with ciclosporin therapy included the following: weight gain (14%), dental tartar and gingivitis (10%), otitis (4%), chronic diarrhoea (4%), inflammatory bowel disease with indolent gastrointestinal lymphoma (2%), urinary tract infection (2%), cataract (2%), elevated liver enzymes (2%), hyperthyroidism and renal failure (2%) and transient inappropriate urination (2%). Some cats experienced multiple adverse events. Case-control studies are needed to prove cause and effect of ciclosporin with regard to these adverse events.

Efficacy of tylosin tartrate on canine Staphylococcus intermedius isolates in vitro.

In the past 5 years, the incidence of canine skin infections caused by resistant strains of Staphylococcus (pseud)intermedius has increased. Many older antibiotics are used to treat these infections because the sensitivity can be demonstrated in vitro. Additionally, many of these older drugs are efficacious and unlikely to induce multidrug resistance. More than a decade ago, the antibiotic tylosin tartrate was reported to be efficacious in vitro and in vivo against Staphylococcus intermedius. The purpose of this study was to determine whether S. (pseud)intermedius isolated from untreated pyoderma cases at veterinary referral centers across the United States are sensitive in vitro to this antibiotic. Minimum inhibitory concentrations for tylosin tartrate and other commonly used antibiotics were determined for 103 isolates. Most (82.61%) of the isolates not exposed to antibiotics in the 3 months before submission were sensitive to tylosin tartrate. These findings suggest that tylosin tartrate warrants further study as a first-line option for the treatment of dogs initially presenting with pyoderma.

Hypothyroidism in a dog associated with trimethoprimsulphadiazine therapy.

Abstract Hypothyroidism and a neuromuscular disorder developed in a 4-year-old Golden Retriever after it received potentiated sulphonamide and metronidazole for 18 and 14 weeks, respectively. Serum total T4 concentrations were non-detectable before and 6 h after exogenous administration of 4IU bovine thyroidstimulating hormone. Thyroid gland biopsy revealed changes consistent with diffuse hyperplastic goitre. Serum T4 concentrations were normal 7 days after discontinuation of therapy. The long-term trimethoprim-sulphadiazine therapy was considered the most likely cause of this dog's hyperplastic goitre. The cause of the neuromuscular disorder was not determined. It is recommended that discontinuation of potentiated sulphonamide takes place at least 7 days prior to any assessment of thyroid function. Résumé- Une hypothyroïdie et des troubles neuromusculaires sont observés sur un Golden Retriever de 4 ans, après une thérapeutique à base de sulfonamides potentialisés et de métronidazole, respectivement de 18 et 14 semaines. Des concentrations sériques de T4 totale ne sont pas détectables avant et après 6 heures d'une stimulation à la TSH bovine (4 UI). Des biopsies de la thyroïde montrent un goitre hyperplasique diffus. Les concentrations sériques de T4 sont de nouveau normales 7 jours après l'arrêt du traitement. L'administration à long terme de triméthoprim-sulphadiazine semble être la cause la plus vraisemblable du goitre hyperplasique. La cause des troubles neuromusculaires n'a pas été déterminée. Il est recommandé d'arrêter l'administration de sulfonamides potentialisés au moins 7 jours avant une exploration de la function thyroïdienne. [Torres, S.M.F. Hypothyroidism in a dog associated with triméthoprim-sulphadiazine therapy (Hypothyroi'die en relation avec un traitement triméthoprim-sulphadiazine chez un chien). Veterinary Dermatology 1996; 7: 105-108.] Resumen Un perro Golden Retriever de 4 años desarrolló hipotiroidismo y una afección neuromuscular después de recibir un tratamiento con sulfonamidas potenciadas y metronidazol 18 y 14 semanas, respectivamente. Las concentraciones séricas totales de T4 eran indetectables antes y a las 6 horas después de la administración exógena de 4 UI de TSH bovina. La biopsia de tiroides mostró alteraciones indicativas de gota hiperplásica difusa. Las concentraciones séricas de T4 fueron normales a los 7 dias de retirar la terapia. La terapia prolongada con trimetoprim-sulfadiazina fue considerada la causa más probable de gota hiperplásica en este perro. No se determinó la causa del cuadro neuromuscular. Se recomienda retirar la terapia con sulfonamida potenciada al menos 7 días antes de la evaluación de la función tiroidea. [Torres, S.M.F. Hypothyroidism in a dog associated with trimethoprim-sulphadiazine therapy (Hipotiroidismo en un perro asociado a la terapia con trimetoprim-sulfadiazina). Veterinary Dermatology 1996; 7: 105-108.] Zusammenfassung- Hypothyreose und eine neuromuskuläre Störung entwickelten sich bei einem 4 Jahre alten Golden Retriever, nachdem er potenzierte Sulfonamide und Metronidazol über 18 beziehungsweise 14 Wochen erhalten hatte. Die Gesamt T4-Konzentrationen waren vor und 6 Stunden nach exogener Verabreichung von 4 IE bovinen TSH nicht meßbar. Eine Biopsie der Schilddrüse zeigte Veränderungen, die parallel mit diffusem hyperplasischem Kropf auftreten. Die Serum T4-Konzentrationen waren 7 Tage nach Abbruch der Therapie wieder normal. Die Langzeit-Trimethoprim-Sulfadiazin-Therapie wurde als wahr-scheinlichste Ursache dieses hyperplastischen Kropfes beim Hund angesehen. Die Ursache der neuro-muskulären Störung konnte nicht festgestellt werden. Es wird empfohlen, potenzierte Sulfonamide mindestens 7 Tage vor einer überprüfung der Schilddrüsenfunktion abzusetzen. [Torres, S. M. F. Hypothyroidism in a dog associated with trimethoprim-sulphadiazine therapy (Hyperthyreose bei einem Hund in Verbindung mit einer Trimethoprim-Sulfadiazin-Therapie). Veterinary Dermatology 1996; 7: 105-108.].