Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
2.
FASEB J ; 27(3): 1153-66, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23195035

RESUMO

This study explored the novel use of iron oxide (IO) nanoparticles (<20 nm) as a vaccine delivery platform without additional adjuvants. A recombinant malaria vaccine antigen, the merozoite surface protein 1 (rMSP1), was conjugated to IO nanoparticles (rMSP1-IO). Immunizations in outbred mice with rMSP1-IO achieved 100% responsiveness with antibody titers comparable to those obtained with rMSP1 formulated with a clinically acceptable adjuvant, Montanide ISA51 (2.7×10 vs. 1.6×10; respectively). Only rMSP1-1O could induce significant levels (80%) of parasite inhibitory antibodies. The rMSP1-IO was highly stable at 4°C and was amenable to lyophilization, maintaining its antigenicity, immunogenicity, and ability to induce inhibitory antibodies. Further testing in nonhuman primates, Aotus monkeys, also elicited 100% immune responsiveness and high levels of parasite inhibitory antibodies (55-100% inhibition). No apparent local or systemic toxicity was associated with IO immunizations. Murine macrophages and dendritic cells efficiently (>90%) internalized IO nanoparticles, but only the latter were significantly activated, with elevated expression/secretion of CD86, cytokines (IL-6, TNF-α, IL1-b, IFN-γ, and IL-12), and chemokines (CXCL1, CXCL2, CCL2, CCL3, CCL4, and CXCL10). Thus, the IO nanoparticles is a novel, safe, and effective vaccine platform, with built-in adjuvancy, that is highly stable and field deployable for cost-effective vaccine delivery.


Assuntos
Portadores de Fármacos/farmacologia , Compostos Férricos/farmacologia , Vacinas Antimaláricas/farmacologia , Proteína 1 de Superfície de Merozoito/farmacologia , Nanopartículas , Animais , Aotidae , Citocinas/imunologia , Células Dendríticas/imunologia , Portadores de Fármacos/química , Compostos Férricos/química , Humanos , Imunização , Macrófagos/imunologia , Malária/imunologia , Malária/prevenção & controle , Vacinas Antimaláricas/imunologia , Proteína 1 de Superfície de Merozoito/imunologia , Camundongos , Vacinas Sintéticas/farmacologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA