Different factors limit early- and late-season windows of opportunity for monarch development.

Seasonal windows of opportunity are intervals within a year that provide improved prospects for growth, survival, or reproduction. However, few studies have sufficient temporal resolution to examine how multiple factors combine to constrain the seasonal timing and extent of developmental opportunities. Here, we document seasonal changes in milkweed (Asclepias fascicularis)-monarch (Danaus plexippus) interactions with high resolution throughout the last three breeding seasons prior to a precipitous single-year decline in the western monarch population. Our results show early- and late-season windows of opportunity for monarch recruitment that were constrained by different combinations of factors. Early-season windows of opportunity were characterized by high egg densities and low survival on a select subset of host plants, consistent with the hypothesis that early-spring migrant female monarchs select earlier-emerging plants to balance a seasonal trade-off between increasing host plant quantity and decreasing host plant quality. Late-season windows of opportunity were coincident with the initiation of host plant senescence, and caterpillar success was negatively correlated with heatwave exposure, consistent with the hypothesis that late-season windows were constrained by plant defense traits and thermal stress. Throughout this study, climatic and microclimatic variations played a foundational role in the timing and success of monarch developmental windows by affecting bottom-up, top-down, and abiotic limitations. More exposed microclimates were associated with higher developmental success during cooler conditions, and more shaded microclimates were associated with higher developmental success during warmer conditions, suggesting that habitat heterogeneity could buffer the effects of climatic variation. Together, these findings show an important dimension of seasonal change in milkweed-monarch interactions and illustrate how different biotic and abiotic factors can limit the developmental success of monarchs across the breeding season. These results also suggest the potential for seasonal sequences of favorable or unfavorable conditions across the breeding range to strongly affect monarch population dynamics.

Thermal sensitivity and seasonal change in the gut microbiome of a desert ant, Cephalotes rohweri.

Microorganisms within ectotherms must withstand the variable body temperatures of their hosts. Shifts in host body temperature resulting from climate change have the potential to shape ectotherm microbiome composition. Microbiome compositional changes occurring in response to temperature in nature have not been frequently examined, restricting our ability to predict microbe-mediated ectotherm responses to climate change. In a set of field-based observations, we characterized gut bacterial communities and thermal exposure across a population of desert arboreal ants (Cephalotes rohweri). In a paired growth chamber experiment, we exposed ant colonies to variable temperature regimes differing by 5°C for three months. We found that the abundance and composition of ant-associated bacteria were sensitive to elevated temperatures in both field and laboratory experiments. We observed a subset of taxa that responded similarly to temperature in the experimental and observational study, suggesting a role of seasonal temperature and local temperature differences amongst nests in shaping microbiomes within the ant population. Bacterial mutualists in the genus Cephaloticoccus (Opitutales: Opitutaceae) were especially sensitive to change in temperature-decreasing in abundance in naturally warm summer nests and warm growth chambers. We also report the discovery of a member of the Candidate Phlya Radiation (Phylum: Gracilibacteria), a suspected epibiont, found in low abundance within the guts of this ant species.

Predicted Asymmetrical Effects of Warming on Nocturnal and Diurnal Soil-Dwelling Ectotherms.

AbstractClimate is expected to have broad effects on ecological communities, but this occurs in the context of significant daily temperature variation in many localities. Because many ectotherms can restrict activity to thermally suitable places and times, daily temperature variation offers the potential to buffer impacts of warming. Using thermal activity data from a montane ground-nesting ant community, we explore how a simulated increase in temperature is expected to alter the duration of suitable activity windows. Counterintuitively, we found that simulated warming lengthens activity times for cold-active species and shortens activity times for warm-active species. We explain this result through a simulation model in which time elapsed within a range of suitable temperatures is considered as an additive resource. Fundamentally, our model results rely on the fact that the mathematical function that relates time to temperature through a day (the Parton-Logan function) is concave before and after noon and convex through the night. These properties are common across terrestrial environments with characteristic deceleration in temperature near both the daily maximum and the daily minimum. Our results suggest that the time of day during which an animal's activity temperatures occur may be an important but rarely considered feature of natural history that contributes to the predicted impact of climate change. Thermally restricted diurnal species may need to compensate for shortened daily activity windows through means such as seasonal shifts or expansions, broadened activity temperatures, or range shifts.

Culturable bacteria are more common than fungi in floral nectar and are more easily dispersed by thrips, a ubiquitous flower visitor.

Variation in dispersal ability among taxa affects community assembly and biodiversity maintenance within metacommunities. Although fungi and bacteria frequently coexist, their relative dispersal abilities are poorly understood. Nectar-inhabiting microbial communities affect plant reproduction and pollinator behavior, and are excellent models for studying dispersal of bacteria and fungi in a metacommunity framework. Here, we assay dispersal ability of common nectar bacteria and fungi in an insect-based dispersal experiment. We then compare these results with the incidence and abundance of culturable flower-inhabiting bacteria and fungi within naturally occurring flowers across two coflowering communities in California across two flowering seasons. Our microbial dispersal experiment demonstrates that bacteria disperse via thrips among artificial habitat patches more readily than fungi. In the field, incidence and abundance of culturable bacteria and fungi were positively correlated, but bacteria were much more widespread. These patterns suggest shared dispersal routes or habitat requirements among culturable bacteria and fungi, but differences in dispersal or colonization frequency by thrips, common flower visitors. The finding that culturable bacteria are more common among nectar sampled here, in part due to superior thrips-mediated dispersal, may have relevance for microbial life history, community assembly of microbes, and plant-pollinator interactions.

Predator Diversity and Thermal Niche Complementarity Attenuate Indirect Effects of Warming on Prey Survival.

AbstractClimate warming has broad-reaching effects on communities. Although much research has focused on direct abiotic effects, indirect effects of warming mediated through biotic interactions can be of equal or greater magnitude. A body of theoretical and empirical work has developed examining the effects of climate warming on predator-prey interactions, but most studies have focused on single predator and prey species. We develop a model with multiple predator species using simulated and measured realized thermal niches from a community of ants to examine the influence of predator diversity and other community thermal traits on the indirect effects of climate warming on prey survival probability. We find that predator diversity attenuates the indirect effect of climate warming on prey survival probability and that sufficient variation of predator thermal optima, closer prey and mean predator thermal optima, and higher predator niche complementarity increases the attenuation effect of predator diversity. We therefore predict that more diverse and complementary communities are likely more affected by direct versus indirect effects of climate warming, and vice versa for less diverse and complementary communities. If general, these predictions could lessen the difficulty of predicting the effects of climate warming on a focal species of interest.

Artificial Light Increases Local Predator Abundance, Predation Rates, and Herbivory.

Human activity is rapidly increasing the radiance and geographic extent of artificial light at night (ALAN) leading to alterations in the development, behavior, and physiological state of many organisms. A limited number of community-scale studies investigating the effects of ALAN have allowed for spatial aggregation through positive phototaxis, the commonly observed phenomenon of arthropod movement toward light. We performed an open field study (without restricted arthropod access) to determine the effects of ALAN on local arthropod community composition, plant traits, and local herbivory and predation rates. We found strong positive phototaxis in 10 orders of arthropods, with increased (159% higher) overall arthropod abundance under ALAN compared to unlit controls. The arthropod community under ALAN was more diverse and contained a higher proportion of predaceous arthropods (15% vs 8%). Predation of immobilized flies occurred 3.6 times faster under ALAN; this effect was not observed during the day. Contrary to expectations, we also observed a 6% increase in herbivory under ALAN. Our results highlight the importance of open experimental field studies in determining community-level effects of ALAN.

The timing of leaf damage affects future herbivory in mountain sagebrush (Artemisia tridentata).

Many plants respond to herbivory by increasing expression of defensive traits. The defensive response of plants can vary depending on plant condition, seasonality, and time of day. Due to a lack of field-based studies, it is unclear how temporal variability in defensive response may alter future rates of herbivory within ecological communities. In a series of simulated herbivory experiments, I quantified how the timing of leaf damage in mountain sagebrush (Artemisia tridentata ssp. vaseyana) affects future herbivory. An identical leaf damage treatment was applied across 12 time windows to test how the effectiveness of response to herbivore damage changes along three interacting temporal scales: diel, seasonal, and annual. In contrast to several studies demonstrating induced resistance to herbivory in sagebrush, prevention of future herbivory was only detected following summer afternoon leaf damage in one of three years. These findings suggest that the timing of experimental leaf damage is one of many factors contributing to variability in field-based plant defensive induction studies.

Predicting species-specific responses of fungi to climatic variation using historical records.

Although striking changes have been documented in plant and animal phenology over the past century, less is known about how the fungal kingdom's phenology has been changing. A few recent studies have documented changes in fungal fruiting in Europe in the last few decades, but the geographic and taxonomic extent of these changes, the mechanisms behind these changes, and their relationships to climate are not well understood. Here, we analyzed herbarium data of 274 species of fungi from Michigan to test the hypotheses that fruiting times of fungi depend on annual climate and that responses depend on taxonomic and functional groups. We show that the fungal community overall fruits later in warmer and drier years, which has led to a shift toward later fruiting dates for autumn-fruiting species, consistent with existing evidence. However, we also show that these effects are highly variable among species and are partly explained by basic life-history characteristics. Resulting differences in climate sensitivities are expected to affect community structure as climate changes. This study provides a unique picture of the climate dependence of fungal phenology in North America and an approach for quantifying how individual species and broader fungal communities will respond to ongoing climate change.