RESUMO
A method utilizing immobilized major group rhinoviruses and biotinylated soluble intercellular adhesion molecule 1 has been developed for the detection of capsid binders. Binding measurements indicate a loss in relative affinity of biotinylated soluble intercellular adhesion molecule 1 for rhinoviruses by capsid binders. This method and a new class of capsid binders are discussed.
Assuntos
Capsídeo/metabolismo , Moléculas de Adesão Celular/metabolismo , Picornaviridae/metabolismo , Rhinovirus/metabolismo , Tiazóis/análise , Tiazóis/metabolismo , Biotina/metabolismo , Ensaio de Imunoadsorção Enzimática , Células HeLa , Humanos , Molécula 1 de Adesão Intercelular , SolubilidadeRESUMO
Soluble intercellular adhesion molecule-1 (sICAM-1) was shown to be the receptor for the major subgroup of rhinoviruses. It was demonstrated that this molecule can inhibit the binding and subsequent infection of target cells by rhinoviruses belonging to the major but not the minor subgroup. The data reported now describe an ELISA-based system utilizing biotinylated sICAM-1 as a means of detecting rhinoviruses belonging to the major subgroup.