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1.
J Appl Microbiol ; 125(2): 457-467, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29729069

RESUMO

AIM: Thiss study was conducted to investigate the possible role of a compatible solute from radio-halophilic bacterium against desiccation and ultra-violet radiation-induced oxidative stress. METHODS AND RESULTS: Nine different radio-resistant bacteria were isolated from desert soil, where strain WMA-LM19 was chosen for detailed studies on the basis of its high tolerance to ultraviolet radiation among all these isolates. Here, 16S rRNA gene sequencing indicated the bacterium was closely related to Stenotrophomonas sp. (KT008383). A bacterial milking strategy was applied for extraction of intracellular compatible solutes in 70% (v/v) ethanol, which were purified by High Performance Liquid Chromatography (HPLC). The compound was characterized as ectoine by 1 H and 13 C Nuclear Magnetic Resonance (NMR), and Mass Spectrometry (MS). Ectoine inhibited oxidative damage to proteins and lipids in comparison to the standard ascorbic acid. It also demonstrated more efficient prevention (54·80%) against lysis to erythrocytes membrane by surface active agents than lecithin. Furthermore, a high level of ectoine-mediated protection of bovine serum albumin against ionizing radiation (1 500-2 000Jm-2 ) was observed, as indicated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. CONCLUSION: The results indicated that ectoine from Stenotrophomonas sp. WMA-LM19 can be used as a potential mitigator and radio-protective agent to overcome radiation- and salinity-mediated oxidative damages in extreme environment. SIGNIFICANCE AND IMPACT OF THE STUDY: Due to its anti-oxidant properties, ectoine from a radio-halophilic bacterium might be used in sunscreen formulation for protection against UV-induced oxidative stress.


Assuntos
Diamino Aminoácidos/farmacologia , Protetores contra Radiação/farmacologia , Stenotrophomonas/química , Raios Ultravioleta/efeitos adversos , Animais , Membrana Eritrocítica/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Microbiologia do Solo , Stenotrophomonas/genética
2.
J Nat Prod ; 64(9): 1183-90, 2001 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-11575953

RESUMO

Extracts of the endemic nudibranch Leminda millecra collected in Algoa Bay, South Africa, yielded four known metabolites, millecrones A (1) and B (2), isofuranodiene (5), and (+)-8-hydroxycalamenene (9), and nine new compounds, algoafuran (7), cubebenone (8), and a series of seven triprenylquinones and hydroquinones (21-27). A subsequent GC-MS survey of extracts of 21 of L. millecra's possible octocoral prey species in Algoa Bay identified the soft coral Alcyonium fauri as the source of 1 and the gorgonian Leptogorgia palma as the species producing 2 and 8.


Assuntos
Cnidários/química , Furanos/isolamento & purificação , Moluscos/química , Sesquiterpenos/isolamento & purificação , Animais , Cromatografia Líquida de Alta Pressão , Furanos/química , Cromatografia Gasosa-Espectrometria de Massas , Espectroscopia de Ressonância Magnética , Conformação Molecular , Estrutura Molecular , Quinonas/química , Quinonas/isolamento & purificação , Sesquiterpenos/química , África do Sul , Espectrofotometria Infravermelho , Estereoisomerismo
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