Scandium, yttrium, and lanthanide occurrence in Cantharellus cibarius and C. minor mushrooms.

There is a dearth of data on rare earth elements (REE), yttrium and scandium in foods which extends also to baseline datasets for edible wild mushrooms, though this has started to change in the last decade. Concentrations and shale normalized patterns of REE and Y (REY) were studied by using inductively coupled plasma-quadrupole mass spectrometer in 22 pools (2235 specimens) of Cantharellus cibarius (Golden Chanterelle) collected in Poland and also a pool of C. minor (Small Chanterelle) (153 specimens) from Yunnan (Chinese Province). The total REY plus Sc varied in C. cibarius from 10 to 593 µg kg-1 dw whereas that for the Yunnan's C. minor was 2072 µg kg-1 dw. C. minor from Yunnan has higher REY and Sc compared to the C. cibarius. Sc concentrations in twenty C. cibarius pools were below 1 µg kg-1 dw, but 17 and 27 µg kg-1 dw were detected at the other two sites and 66 µg kg-1 dw was detected in C. minor. The median Y content of C. cibarius and C. minor was 22 µg kg-1 dw and 200 µg kg-1 dw. The difference in REY and Sc concentrations and shale normalized patterns between mushrooms from Poland and Yunnan seems to reflect the regional difference in concentration and composition of these elements in the soil bedrock.

Occurrence, bio-concentration and distribution of rare earth elements in wild mushrooms.

Using validated methodology, this study explores the bioconcentration potential and status of rare earth elements (REE) and yttrium (Y) in wild mushrooms collected from Belarus, China and Poland and in the associated forest topsoil. Baseline data for REE and Y distributions in the morphological parts of the fruiting bodies of Caloboletus calopus, Cantharellus cibarius, Craterellus cornucopioides, Imleria badia, Laccaria amethystina, Lactifluus piperatus, Leccinum scabrum and Suillus grevillei are presented. REE were in the range of 14 to 42 mg kg-1 dw in forest topsoil and from 35 to 48 mg kg-1 dw in profiled soil layers from the Sobowidz site in Poland. Forest topsoil sampled in Belarus contained 67 mg kg-1 dw. Yttrium concentrations in soil ranged from 2.9 to 10 mg kg-1 dw. The median REE concentration in wild mushrooms was around 200 µg kg-1 dw (20 µg kg-1 fresh weight). This implies negligible dietary intake even for high level consumers. The bioconcentration factors (BCF) of individual REE and Y ranged from 0.0002 to 0.0229, showing bio-exclusion. The BCF tended to be similar for groups of REE (La to Tb and Dy to Lu) depending on the mushroom species and site. REE from Dy to Lu were better bioconcentrated than those from La to Tb. The similarity of the BCFs of individual REE by species at a given site implies the same absorption pathway, although a lower concentration in the topsoil favoured bioconcentration. REE and Y concentrations varied between species as well as within the same species between sites. Their accumulation in mushrooms appears to reflect condition at the site of collection, and may also be species-specific but confirming this would require further investigation of different species, topsoils and sites.

Rare Earth Elements in Boletus edulis (King Bolete) Mushrooms from Lowland and Montane Areas in Poland.

Mining/exploitation and commercial applications of the rare-earth elements (REEs: La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, and Lu) in the past 3 decades have raised concerns about their emissions to the environment, possible accumulation in food webs, and occupational/environmental health effects. The occurrence and distribution of REEs Y and Sc in the fruitbodies of Boletus edulis collected from geographically diverse regions in Poland were studied in 14 composite samples that were derived from 261 whole fruiting bodies. Individual REE median concentrations ranged from 0.4-95 µg kg-1 dry weight (dw). The summed REE concentrations varied widely, with a median value of 310 µg kg-1 dw and a range of 87 to 758 µg kg-1. The Sc and Y median concentrations (dw) were 35 and 42 µg kg-1, respectively. Ce, La, and Nd, with median values of 95, 51, and 32 µg kg-1, respectively, showed the highest occurrence. B. edulis collected from a forested area formerly used as a military shooting range-possibly a historically contaminated site-had an elevated summed REE content of 1796 µg kg-1. REE concentrations were generally low in Polish King Bolete. Dietary intake from a mushroom meal was negligible, posing no health risk to consumers.

Mercury in traditionally foraged species of fungi (macromycetes) from the karst area across Yunnan province in China.

The objective of this study is to better quantify the occurrence, intake, and potential risk from Hg in fungi traditionally foraged in SW China. The concentrations and intakes of Hg were measured from 42 species including a "hard" flesh type polypore fungi and a" soft" flesh type edible species that are used in traditional herbal medicine, collected during the period 2011-2017. Three profiles of forest topsoil from the Zhenyuan site in 2015 and Changning and Dulong sites in 2016 were also investigated. The concentrations of Hg in composite samples of polypore fungi were usually below 0.1 mg kg-1 dry weight (dw) but higher levels, 0.11 ± 0.01 and 0.24 ± 0.00 mg kg-1 dw, were noted in Ganoderma applanatum and Amauroderma niger respectively, both from the Nujiang site near the town of Lanping in NW Yunnan. Hg concentrations in Boletaceae species were usually well above 1.0 mg kg-1 dw and as high as 10 mg kg-1 dw. The quality of the mushrooms in this study in view of contamination with Hg showed a complex picture. The "worst case" estimations showed probable intake of Hg from 0.006 µg kg-1 body mass (bm) ("hard" type flesh) to 0.25 µg kg-1 bm ("soft" flesh) on a daily basis for capsulated products, from 17 to 83 µg kg-1 bm ("soft" flesh) in a meal ("hard" type flesh mushrooms are not cooked while used in traditional herbal medicine after processing), and from 0.042 to 1.7 and 120 to 580 µg kg-1 bm on a weekly basis, respectively. KEY POINTS: • Polypore species were slightly contaminated with Hg. • Hg maximal content in the polypore was < 0.25 mg kg-1 dry weight. • Many species from Boletaceae family in Yunnan showed elevated Hg. • Locals who often eat Boletus may take Hg at a dose above the daily reference dose.

Accumulation of Minerals by Leccinum scabrum from Two Large Forested Areas in Central Europe: Notecka Wilderness and Tuchola Forest (Pinewoods).

Leccinum scabrum sporocarps and associated topsoils from two areas in Poland have been characterized for contents and bioconcentration potential of Ag, Al, Ba, Ca, Cd, Co, Cu, Fe, Hg, K, Mg, Mn, Na, Ni, P, Pb, Rb, Sr and Zn. Topsoil and fruitbody element composition varied between the two study sites, most likely as a result of local soil geochemistry. Element content of the labile fraction in topsoil from both sites followed the 'pseudo-total' fraction and median values (mg kg-1 dry matter) were: K 380 and 340, Mg 760 and 840, P 1100 and 920, Al 3800 and 8100, Ag 0.31 and 0.28, Ba 28 and 37, Ca 920 and 790, Cd 0.23 and 0.23, Co 2.0 and 1.7, Cu 3.2 and 3.6, Fe 2800 and 6300, Mn 280 and 180, Na 99 and 110, Ni 7.8 and 8.8, Pb 12 and 18, Rb 1.3 and 2.1, Sr 4.8 and 4.0 and Zn 22 and 19, respectively. Only for some elements such as K, Mg, Al, Ag, Ca, Co, Mn, Na, Ni, Sr and Zn we found concentration differences between the two study sites for the caps of sporocarps. With the exception of Al, Mn, Na and Pb, stipes showed a similar tendency. Caps had a higher concentration of K, Rb, P, Mg, Al, Ag, Cu, Fe, Zn, Cd, Pb and Ni compared to stipes, while Na, Ba and Sr contents were higher in stipes. The comparison of soil and fruitbody concentrations indicates that L. scabrum bioconcentrate some elements while others are bioexcluded.

Inorganic elemental concentrations in birch bolete mushroom (Leccinum scabrum) and top soil: contamination profiles, bioconcentation and annual variations.

Analysis of inorganic and organic contaminants in foodstuffs aids in understanding the human exposure to these compounds via consumption. In this study, an edible mushroom species (Leccinum scabrum) and top soil samples were analysed for essential and toxic substances including phosphorus and inorganic elements over a period of three fruiting seasons. Analysis of silver (Ag), aluminium (Al), barium (Ba), calcium (Ca), cadmium (Cd), cobalt (Co), copper (Cu), iron (Fe), mercury (Hg), potassium (K), magnesium (Mg), manganese (Mn), sodium (Na), nickel (Ni), phosphorus (P), lead (Pb), rubidium (Rb), strontium (Sr) and zinc (Zn) in mushrooms and topsoil were performed using inductively coupled plasma optical emission spectroscopy (ICP-OES) with ultrasonic cross flow nebulizer. Total mercury was determined by cold-vapour atomic absorption spectroscopy (CV-AAS). The results exhibited wide variation in concentrations of metals between soil and mushroom (cap and stipes) during three fruiting seasons. Positive bioconcentration factors (BFCs) indicate on bioaccumulation of several metals including, Cd, Cu, Hg, K, Mg, Na, P, Rb and Zn in caps and stipes of fruitbodies of this mushroom, while other metals such as Al, Ba, Ca, Co, Fe, Mn, Ni, Pb and Sr were not exhibiting significant positive BFCs. Over a period studied, the caps were characterised by different (p < 0.05) concentrations of Al, Co, Cu, Hg, Mn, Ni, P, Pb and Sr. Contamination profiles, temporal fluctuations, BCFs should be taken into consideration when assessing the nutritional value of this mushroom.

Bio-concentration potential and associations of heavy metals in Amanita muscaria (L.) Lam. from northern regions of Poland.

Fruiting bodies of Amanita muscaria and topsoil beneath from six background areas in northern regions of Poland were investigated for the concentration levels of Ag, Al, Ba, Ca, Cd, Co, Cu, Fe, Hg, K, Mg, Mn, Na, Rb, Sr, and Zn. In addition, the bioconcentration factors (BCF values) were studied for each of these metallic elements. Similar to studies from other basidiomycetes, A. muscaria showed species-specific affinities to some elements, resulting in their bioconcentration in mycelium and fruiting bodies. This mushroom growing in soils with different levels of the geogenic metallic elements (Ag, Al, Ba, Ca, Co, Cu, Fe, Hg, K, Mg, Mn, Na, Rb, Sr, and Zn) showed signs of homeostatic accumulation in fruiting bodies of several of these elements, while Cd appeared to be accumulated at a rate dependent of the concentration level in the soil substrate. This species is an efficient bio-concentrator of K, Mg, Cd, Cu, Hg, Rb, and Zn and hence also contributes to the natural cycling of these metallic elements in forest ecosystems.

Bio- and toxic elements in mushrooms from the city of Umeå and outskirts, Sweden.

Edible mushrooms (Albatrellus ovinus, Boletus edulis, Clitocybe odora, Gomphidius glutinosus, Leccinum scabrum, Leccinum versipelle, Lycoperdon perlatum, Suillus bovinus, Suillus luteus, and Xerocomus subtomentosus) collected from unpolluted areas of the city of Umeå and its outskirts in the northern part of Sweden were examined for contents of toxic metallic elements (Cd, Pb, and Ag) and essential macro- and microelements (K, Na, Ca, Mg, Cu, Fe, Mn, and Zn) using a validated method and a final measurement by flame atomic absorption spectroscopy (F-AAS). The median values of the toxic metallic element concentrations (in mg kg-1 dry biomass, db) ranged from: 0.12-3.9, 0.46-5.1, and 0.91-6.2 for Ag, Cd and Pb, respectively. For the essential metallic elements, the median values of concentrations ranged from: 24000-58000, 15-2000, 59-610, 520-1900, 2.0-97, 16-150, 15-120, and 4.3-26 mg kg-1 db for K, Na, Ca, Mg, Cu, Zn, Fe, and Mn, respectively. The baseline concentrations of the metallic elements determined in mushrooms were mainly affected by the fungal species. The assessed probable maximal dietary intake of Cd (0.002 mg kg-1 body mass) solely from a mushroom meal was only slightly below a revised value of the tolerable weekly intake for this element, while for Pb (0.003 mg kg-1 body mass) it was tenfold below the provisionally tolerable weekly intake.

Analysis of some metallic elements and metalloids composition and relationships in parasol mushroom Macrolepiota procera.

The aim of the study was to characterise the multi-elemental composition and associations between a group of 32 elements and 16 rare earth elements collected by mycelium from growing substrates and accumulated in fruiting bodies of Macrolepiota procera from 16 sites from the lowland areas of Poland. The elements were quantified by inductively coupled plasma quadrupole mass spectrometry using validated method. The correlation matrix obtained from a possible 48 × 16 data matrix has been used to examine if any association exits between 48 elements in mushrooms foraged from 16 sampling localizations by multivariate approach using principal component (PC) analysis. The model could explain up to 93% variability by eight factors for which an eigenvalue value was ≥1. Absolute values of the correlation coefficient were above 0.72 (significance at p < 0.05) for 43 elements. From a point of view by consumer, the absolute content of Cd, Hg, Pb in caps of M. procera collected from background (unpolluted) areas could be considered elevated while sporadic/occasional ingestion of this mushroom is considered safe. The multivariate functional analysis revealed on associated accumulation of many elements in this mushroom. M. procera seem to possess some features of a bio-indicative species for anthropogenic Pb but also for some geogenic metals.

Specific accumulation of cadmium and other trace elements in Sarcodon imbricatus using ICP-MS with a chemometric approach.

Fungi can effectively accumulate various metallic elements, metalloids and non-metals in fruiting bodies. This study provides information on the accumulation of Ag, As, Ba, Cd, Co, Cs, Cu, Cr, Li, Mn, Ni, Pb, Rb, Sr, V, Tl, U and Zn in the edible mushroom Sarcodon imbricatus (L.) P. Karst. using the technique of inductively coupled plasma - mass spectrometry with a dynamic reaction cell mode. Mushrooms were foraged from four regions in Poland. Baseline concentrations of minerals, expressed in mg kg-1 dry biomass (db), were in the composite samples of caps in the range: for Ag (0.27-0.29), As (1.0-1.9), Ba (0.31-0.45), Cd (4.5-6.3), Co (0.23-1.9), Cu (28-35), Cr (0.19-0.29), Cs (20-38), Li (0.013-0.020), Mn (5.9-8.8), Ni (0.81-1.4), Pb (0.94-1.6), Rb (490-700), Sr (0.14-0.19), Tl (0.058-0.11), U (0.002-0.002), V (0.044-0.054) and Zn (140-160). Concentration levels of Ag, As, Cd, Cs, Pb and Zn were higher in caps than in stipes of S. imbricatus, whereas for other elements the distribution between caps and stipes was nearly equal or for some differed depending on the location. Certainly, the content of toxic Cd in S. imbricatus was elevated (0.45-0.63 mg kg-1 in fresh caps) and therefore eating this mushroom could increase exposure to Cd. In addition, the content of toxic As in S. imbricatus was elevated.

Rare earth elements in parasol mushroom Macrolepiota procera.

This study aimed to investigate occurrence and distribution of 16 rare earth elements (REEs) in edible saprobic mushroom Macrolepiota procera, and to estimate possible intake and risk to human consumer. Mushrooms samples were collected from sixteen geographically diverse sites in the northern regions of Poland. The results showed that for Ce as the most abundant among the RREs in edible caps, the mean concentration was at 0.18±0.29mgkg-1dry biomass. The mean concentration for Σ16 REEs determined in caps of fungus was 0.50mgkg-1dry biomass and in whole fruiting bodies was 0.75mgkg-1dry biomass. From a point of view by consumer, the amounts of REEs contained in edible caps of M. procera could be considered small. Hence, eating a tasty caps of this fungus would not result in a health risk for consumer because of exposure to the REEs.

Bio- and toxic elements in edible wild mushrooms from two regions of potentially different environmental conditions in eastern Poland.

In the present study, the composition of bio-elements (K, Na, Mg, Ca, Fe, Cu, Zn) and toxic elements (Ag, Cd) in seven edible mushrooms from the rural and woodland region of Morag (north-eastern Poland) and the rural and industrial region of the Tarnobrzeska Upland (south-eastern Poland) were investigated using a validated method. The species examined were Boletus edulis, Cantharellus cibarius, Leccinum aurantiacum, Leccinum versipelle, Lycoperdon perlatum, Suillus luteus, and Xerocomus subtomentosus. Final determination was carried out by flame atomic absorption spectroscopy (FAAS) after microwave-assisted decomposition of sample matrices with solutions of concentrated nitric acid in the pressurized polytetrafluoroethylene vessels. The contents of the alkali elements and alkali earth elements were determined in the species surveyed. The alkali elements, earth alkali elements, and transition metals (Ag, Cu, Zn, Fe, and Mn) were at typical concentrations as was determined for the same or similar species elsewhere in Poland and Europe. The results may suggest a lack of local and regional emissions of those metallic elements from industrialization of some sites in the Tarnobrzeska Plain. Cadmium was at elevated concentrations in L. versipelle from the Tarnobrzeska Plain but the reason-pollution or geogenic source-was unknown, while it was at typical concentrations in other species.