RESUMO
A Gram-negative, microaerophilic slender rod, measuring approximately 10 mum long and approximately 1 microm wide, isolated from the gastric mucosa of a cat and designated strain M50(T), was subjected to a polyphasic taxonomic study. Despite its apparent lack of helical coils, the organism showed a corkscrew-like motion by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril coiled around the body. Strain M50(T) grew preferably on biphasic culture plates or on very moist agar. Coccoid forms predominated in cultures older than 4 days as well as in growth obtained on dry agar plates. The strain grew at 37 degrees C, but not at 25 or 42 degrees C and exhibited urease, oxidase and catalase activities. On the basis of 16S rRNA gene sequence analysis, the novel isolate was identified as a member of the genus Helicobacter and showed about 98 to 99 % sequence similarity to Helicobacter felis, Helicobacter bizzozeronii, Helicobacter salomonis, Helicobacter cynogastricus and 'Candidatus Helicobacter heilmannii', five highly related species previously detected in the feline or canine gastric mucosa. Protein profiling of strain M50(T) using SDS-PAGE revealed a pattern different from those of other Helicobacter species of mammalian gastric origin. Additionally, the urease and HSP60 gene sequences of strain M50(T) were different from those of H. felis, H. bizzozeronii, H. salomonis, H. cynogastricus and 'Ca. H. heilmannii'. It is thus proposed that strain M50(T) (=LMG 23839(T)=CCUG 53816(T)) represents a novel species within this genus, for which the name Helicobacter baculiformis sp. nov. is proposed.
Assuntos
Doenças do Gato/microbiologia , Mucosa Gástrica/microbiologia , Infecções por Helicobacter/veterinária , Helicobacter/classificação , Animais , Técnicas de Tipagem Bacteriana , Gatos , Chaperonina 60/genética , Chaperonina 60/metabolismo , DNA Bacteriano/análise , Genes de RNAr , Helicobacter/genética , Helicobacter/isolamento & purificação , Infecções por Helicobacter/microbiologia , Dados de Sequência Molecular , Fenótipo , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Especificidade da Espécie , Urease/genética , Urease/metabolismoRESUMO
Listeria monocytogenes strains possess short repetitive extragenic palindromic (REP) elements and enterobacterial repetitive intergenic consensus (ERIC) sequences. We used repetitive element sequence-based PCR (rep-PCR) to evaluate the potential of REP and ERIC elements for typing L. monocytogenes strains isolated from humans, animals, and foods. On the basis of rep-PCR fingerprints, L. monocytogenes strains were divided into four major clusters matching origin of isolation. rep-PCR fingerprints of human and animal isolates were different from those of food isolates. Computer evaluation of rep-PCR fingerprints allowed discrimination among the tested serotypes 1/2a, 1/2b, 1/2c, 3b, and 4b within each major cluster. The index of discrimination calculated for 52 epidemiologically unrelated isolates of L. monocytogenes was 0.98 for REP- and ERIC-PCR. Our results suggest that rep-PCR can provide an alternative method for L. monocytogenes typing.
Assuntos
Técnicas de Tipagem Bacteriana , DNA Bacteriano/análise , Listeria monocytogenes/classificação , Reação em Cadeia da Polimerase/métodos , Sequências Repetitivas de Ácido Nucleico/genética , Animais , Impressões Digitais de DNA , DNA Bacteriano/genética , Estudos de Avaliação como Assunto , Microbiologia de Alimentos , Humanos , Listeria monocytogenes/genética , Listeria monocytogenes/isolamento & purificação , FilogeniaRESUMO
This paper deals with a Brucella infection in a laboratory worker and his wife. The diagnosis of brucellosis was proved by isolating Brucella melitensis, biotype 2, from blood cultures and by the standard serum agglutination test. In the absence of any alternative epidemiologic information, the authors consider this case to be an example of interhuman infection.
Assuntos
Brucelose/transmissão , Adulto , Testes de Aglutinação , Anticorpos Antibacterianos/análise , Brucella/imunologia , Brucella/isolamento & purificação , Brucelose/diagnóstico , Brucelose/genética , Feminino , Humanos , Masculino , Especificidade da EspécieRESUMO
Two cases of infection with Campylobacter fetus subspecies intestinalis are reported. In a 46-year-old farmer the development of septicaemic campylobacteriosis with acute gastroenterocolitis was favoured by malnutrition due to chronic alcoholism. After treatment with erythromycin the patient recovered completely. The second patient, a 40-year-old worker with meningitis, recovered after combined streptomycin-erytro-mycin therapy. In both patients antibodies against homologous and reference strains of Campylobycter fetus subspecies intestinalis, serotype 1 (Mitscherlich) were demonstrated in titres ranging from 1:16 to 1:64 by type the complement-fixation test.
