Valorization of biowastes as fermentative substrate for production of Exiguobacterium sp. GM010 pigment and toxicity effect in rats.

Bioconversion of biowastes chicken feather (CF), prawn carapace (PC), fish scale (FS), and corncob (CC) were used for Exiguobacterium sp. GM010 pigment production to reduce environmental pollution. Maximum pigment was produced in 4 % PC hydrolysate medium at pH 8 and 30 °C (0.831 Absorption Unit-AUmL-1) compared to other hydrolysate. Biomass (1061.19 ± 26.14 mg/100 mL) and pigment yield (34.26 ± 0.62 mg/100 mL) were higher in PC medium. In CF + PC hydrolysate combination, biomass and pigment yield was 890.58 ± 11.5 mg/100 mL and 13.94 ± 0.17 mg/100 mL, respectively. Carbon and nitrogen ratio in the medium influenced pigment production. The UV-visible spectrum showed absorption peak at 357, 466, and 491 nm. Further hue angle (77-72) and chroma values (8.68-11.38) distributed over yellowish-orange region of CIELAB spectrum indicated carotenoid like characteristics. Wistar rats fed with pigment (2000 mg/kg bw) did not show sign of toxicity in haematological, biochemical and histopathological analysis. Therefore, pigment produced by recycling the biowastes promotes sustainable bioprocess and circular bioeconomy.

Dual Mode of Action of Talaromyces purpureogenus CFRM02 Pigment to Ameliorate Alcohol Induced Liver Toxicity in Rats.

Talaromyces purpureogenus CFRM02 pigment exhibited antioxidant activity by scavenging free radicals. The alcohol feeding leads to free radical generation causing pathophysiological processes of alcoholic liver disease (ALD) and alcoholic hepatitis. T. purpureogenus CFRM02 pigment administered to rats ameliorated the ALD by scavenging ROS. The haematological analysis revealed the increased neutrophil circulation. The neutrophil infiltration was observed in the hepatocytes of the rats fed with pigment (600 mg/kg body weight). The increase in the number of neutrophils helps in liver regeneration caused by alcoholic hepatitis. The dual mechanism of action of pigment, antioxidant and liver regeneration through neutrophil production is attributed to alleviate the ALD. These results suggested that T. purpureogenus CFRM02 pigment represents the protective and therapeutic strategy against ALD.