[Serological method of detecting the antigens of the causative agent of Sonne dysentery in lysates of the fecal inoculates from patients]. / Serologicheskii metod obnaruzhenii antigenov vozbuditelia dizenterii Zonne v lizatakh posevov isprazhenii bol'nykh.

Serological method of detection of Sh. sonnei antigens in the lysates of the patients, fecal cultures is suggested and approved. In the majority of cases of the results of bacteriological and serological methods of study of the feces coincided. Data confirming the specificity of the antibody neutralization test (ANT) in Sonne dysentery are presented. In connection with detection of the screening action of the Vi-antigen of typhoid bacilli there were elaborated additional methods for verifying the specificity of the ANT results. It is recommended to keep agar plates after selection of suspicious colonies during the bacteriological test; the lysate of the microbial crop should be additionally subjected to the ANT, this considerably increasing the percentage of laboratory confirmations of dysentery caused by Sh. sonnei.

[Comparative study of the virulence of the Shigella isolated from bacterial carriers and from acute dysentery patients]. / Sravnitel'noe izuchenie virulentnosti shigell, vydelennykh ot bakterionositelei i bol'nykh ostroi dizenteriei.

The authors present the results of study of the virulence of shigellae isolated from carriers and patients suffering from acute dysentery, on a continuous culture of E1 and Hep-2 cells. The virulence of shigellae isolated from carriers displayed no significant difference from the virulence of shigellae isolated from patients with a mild and moderately severe forms of dysentery. In the patient's organism shigellae were capable of retaining the initial virulence for a long time, despite the treatment and the influence of the macroorganism's protective factors. The authors believe that this was connected with the capacity of Shigellae to parasitic life in the cells of human intestinal epithelium, and, apparently, played a definite role in the formation of carrier state.

[Microflora of the intestine and biliary tracts in patients with viral hepatitis]. / Mikroflora kishechnika i zhelchevyvodiashchikh putei u bol'nykh virusnym gepatitom

The authors present the results of studying the microflora if the intestine and of the biliary tracts in patients with viral hepatitis; dysbacteriosis was revealed in 53% of the cases. This percentage proved to decrease with clinical recovery. Bile proved to be often contaminated with microbes -- 77% of the samples; this was particularly true in cases with cholestatic variant of the disease and in cases of moderate severity. The fact of prolonged habitation of the microorganisms in the bile system is of importance -- 68% of the patients still had microbial flora in the gall bladder and the biliary tracts by the time of discharge from the hospital.

[Study of the antigenic structure of Salmonella by means of immunoelectrophoresis]. / Izuchenie antigennoi struktury sal'monell metodom immunoélektroforeza

The method of immunoelectrophoretic analysis was applied to the study of the antigenic structure of 180 freshly-isolated and laboratory cultures of salmonellae belonging to 29 serological types. A complicated set of specific and common antigens of bacteria was revealed in direct and cross experiments with the use of homologous and heterogenous antisera; immunophoregrams were drawn for each of the serological type under study. General regularities in immunophoretic, diffuse and serological characteristics of the individual antigens of salmonellae were established. It was shown that different serological types of salmonellae possessed surface K antigens along with the O and H antigens. On immunophoregrams K-antigens of salmonellae were revealed in the form of a bright precipitation arch in the central or the anode zone; O antigens gave a characteristic two-component precipitation line in the cathode zone of the phoregram.