RESUMO
Treatment with richlocaine alone and, especially, in combination with antihypoxant energostim decreased the total content of hydroxyproline in the ischemic skin flap on day 3 after excision. Combination therapy with richlocaine and energostim normalized the redox potential in the energy supply system, improved antioxidant protection, and promoted the recovery of a balance between various components in the antioxidant system. These changes were not accompanied enhanced production of malonic dialdehyde. Our results suggest that combination therapy with richlocaine and energostim maintains the adaptive reserves of detoxifying systems in keratinocytes and prevents endotoxemia. Richlocaine primarily stimulates glycolytic synthesis of ATP, activates nonmitochondrial antioxidant enzymes, and increases RNase activity in lysosomes.
Assuntos
Antioxidantes/farmacologia , Hipóxia , Isquemia , Queratinócitos/efeitos dos fármacos , Queratinócitos/patologia , Malondialdeído/análogos & derivados , Piperidinas/farmacologia , Trifosfato de Adenosina/química , Animais , Antioxidantes/química , Transporte de Elétrons , Endotoxemia/metabolismo , Glicólise , Queratinócitos/metabolismo , Lisossomos/metabolismo , Masculino , Malondialdeído/metabolismo , Mitocôndrias/patologia , Oxirredução , Ratos , Ribonucleases/metabolismo , Pele/patologia , Fatores de TempoRESUMO
The local anesthetic richlocaine decreased the area of necrosis in the skin flap under conditions of reduced blood flow by 29.5%. Improved survival of skin flap after richlocaine treatment alleviated endogenous intoxication, reduced secondary inflammatory reaction, improved liver function, and normalized the ratio between vasoconstricting and vasodilating prostaglandins. This effect was most pronounced after combination therapy with richlocaine and direct-action antihypoxant energostim.
Assuntos
Antioxidantes/farmacologia , Citocromos c/farmacologia , Endotoxemia/tratamento farmacológico , Inosina/farmacologia , NAD/farmacologia , Piperidinas/farmacologia , Pele/efeitos dos fármacos , Pele/patologia , Alanina Transaminase/metabolismo , Animais , Aspartato Aminotransferases/metabolismo , Sobrevivência Celular , Combinação de Medicamentos , Endotoxemia/metabolismo , Eritrócitos/metabolismo , Histamina/metabolismo , Hidroxiprolina/química , Hipóxia , Inflamação , Queratinócitos/metabolismo , Lactatos/metabolismo , Masculino , Necrose , Ratos , Fluxo Sanguíneo Regional , Serotonina/metabolismo , Retalhos Cirúrgicos/patologia , Fatores de Tempo , Vasodilatadores/farmacologiaRESUMO
We studied morphological characteristics of the regenerative process in gingival tissues during therapy of chronic periodontitis. Energostim stimulated macrophageal reaction and promoted neoangiogenesis in the inflammatory infiltrate. It was not observed after traditional drug therapy. Energostim promoted vascularization in regenerating tissues, normalized the structure at a greater area of the lamina propria of the gingiva, and prevented fibrous and sclerotic changes. The volume of histiolymphocytic infiltrates in regenerating gingival tissues decreased after application of intradental splints.