Multi-class, multi-residue determination of 132 veterinary drugs in milk by magnetic solid-phase extraction based on magnetic hypercrosslinked polystyrene prior to their determination by high-performance liquid chromatography-tandem mass spectrometry.

A quantitative multi-class multi-residue analytical method was developed for the determination of veterinary drugs in milk by high-performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS). A total of 132 veterinary drugs investigated belonged to almost 15 classes including sulfonamides, ß-lactams, tetracyclines, quinolones, macrolides, nitrofurans, nitroimidazoles, phenicols, lincosamides, pleuromutilins, macrocyclic lactones, quinoxaline antibiotics, benzimidazoles, anthelmintics, coccidiostats and some others. A magnetic solid-phase extraction procedure was developed using magnetic hypercrosslinked polystyrene (HCP/Fe3O4) for the sample preparation prior to HPLC-MS/MS without deproteinization step. The results indicated recoveries of 85-107% for 14 sulfonamides, 85-120% for 13 ß-lactams, 89-115% for 4 tetracyclines, 82-119% for 14 quinolones, 82-115% for 8 macrolides, 97-109% for 4 nitrofurans, 84-115% for 10 nitroimidazoles, 89-114% for 3 phenicols, 86-111% for 3 lincosamides, 97-102% for 2 pleuromutilins, 72-88% for 4 macrocyclic lactones, 87-104% for 4 quinoxaline antibiotics, 76-119% for 21 benzimidazoles, 79-115% for 12 anthelmintics, 81-118% for 12 coccidiostats and 75-119 % for 5 unclassified drugs, with relative standard deviations (RSDs) of less than 20%, and the LOQs ranged from 0.05 to 1 µg kg-1. This methodology was then applied to field-collected real milk samples and trace levels of some veterinary drugs were detected.

Effect of amines on formation of gold/polyurethane foam nanocomposites and its sensing opportunities.

Effect of amines on formation of gold nanoparticles (AuNPs)/polymer nanocomposites has been observed and studied. Nanocomposites based on polyurethane foam and AuNPs were synthesized by interaction between the polymer modified with sodium borohydride and aqueous solution of tetrachloroauric acid. It has been shown that some amines cause a remarkable decrease of the surface plasmon resonance band of AuNPs in the nanocomposite material. Both aliphatic and aromatic amines as well as amines containing several amino groups were studied. A possible mechanism of the effect is discussed. It is probably based on stabilization of AuNPs with an amine that entails a decrease in the degree of their adsorption on PUF and appearance of the stabilized AuNPs in solution. The decrease of the nanocomposite surface plasmon resonance band is proportional to the concentration of amine in the solution. Based on this effect, a method for the determination of cetylamine, ß-naphthylamine and neomycin in water and medical formulations using a monitor calibrator as a portable household tool is proposed. Under the selected conditions, the detection limits for amines were in the range of 0.7-1.5 µM, the determination ranges were approximately an order of magnitude. The observed color change of the nanocomposite samples also provides a good basis for semiquantitative determinations.