RESUMO
AIM: Comparative evaluation of species identification of microorganisms by MALDI-TOF mass-spectrometry and automatic biochemical analyzer VITEK2 Compact 30. MATERIALS AND METHODS: Species identification of 18,400 isolates of microorganisms (staphylococci, streptococci, enterococci, enterobacteria, nonfermenting gram-negative bacteria, lactobacilli, anaerobes, yeast fungi, neisseriae), isolated from vagina of pregnant and non-pregnant women and from newborns, was carried out. Identification of the isolated microorganisms was carried out by automatic bacteriologic analyzer VITEK2 Compact30 (BioMerieuX, France) and MALDI-TOF-MS analysis method on AutofleXIII (Bruker Daltonics, Germany) mass-spectrometer. RESULTS: Comparative identification of 2005 isolates of microorganisms was carried out. Sequencing of ribosomal RNA was used as a reference method. Authenticity of species identification my MALDI-TOF-MS analysis method was: for staphylococci (95.8%), enterococci (97.5%), enterobacteria (98.4%), nonfermenting gram-negative bacteria (93.6%), ß-hemolytiC staphylococci (93.8%), lactobacilli (92.8%), yeast fungi (99.9%). CONCLUSION: Introduction of MALDI-TOF-MS analysis technology into practical work of microbiological laboratories exceeds previously used methods of microbiological testing in terms of speed; cost and authenticity of identification of a wide spectrum of microorganisms.
Assuntos
Centros Médicos Acadêmicos/métodos , Bactérias/classificação , Técnicas de Tipagem Bacteriana/instrumentação , Fungos/classificação , Técnicas de Tipagem Micológica/instrumentação , RNA Ribossômico/genética , Bactérias/genética , Bactérias/isolamento & purificação , Feminino , Fungos/genética , Fungos/isolamento & purificação , Ginecologia , Humanos , Recém-Nascido , Obstetrícia , Perinatologia , Gravidez , RNA Ribossômico/isolamento & purificação , Análise de Sequência de RNA , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Vagina/microbiologiaRESUMO
In the end of XX century real revolution occurred in field of methods, which can be applied for identification of agents of infections including bacteria. Alongside with cultural diagnostic entered an arsenal of molecular genetic methods and proteomic analysis based on application of physical technologies including matrix activated laser desorption/ionization time-of-flight mass spectrometry - MALDI-ToF MS as well. The international experience of application of MALDI-ToF MS as species identification of microorganisms isolated from clinical samples substantiates high importance of method. The potential possibility of implementing direct indication of bacteria in clinical samples significantly shorten period of implementation of analysis and reveals new resources for implementing in various algorithms of microbiological diagnostic.
RESUMO
We analyzed sensitivity of 123 vaginal lactobacillus strains to antibacterial substances. All lactobacillus strains were sensitive to ampicillin, cefazolin, cefotaxime, and vancomycin, and insensitive to metronidazole, trimethoprim/sulfamethoxazole, and levofloxacin. Lactobacillus strains demonstrated different sensitivity to gentamycin, clindamycin, erythromycin, ciprofloxacin, and tetracycline. The phenomenon of preferential selective influence of antibacterial drugs on the composition of lactobacilli of the vaginal microbiota, in which some lactobacilli survive as part of the vaginal microbiota and have a selective advantage over other types of lactobacilli, should be taken into account during treatment of vaginal infections and dysbiosis.