Foliage of Tropical Trees and Shrubs and Their Secondary Metabolites Modify In Vitro Ruminal Fermentation, Methane and Gas Production without a Tight Correlation with the Microbiota.

Ruminants, mainly cattle, contribute to greenhouse gases (GHG) emissions as methane (CH4) is produced by ruminal fermentation. Hence, various anti-methanogenic feed strategies have been studied, including the use of plants with secondary metabolites. This study evaluated in vitro ruminal fermentation metrics, microbial composition by digital droplet PCR (ddPCR) and the CH4 production of the foliage of several tropical trees and shrubs: Leucaena leucocephala, Moringa oleifera, Albizia lebbeck, Enterolobium cyclocarpum, Piscidia piscipula, Brosimum alicastrum, Lysiloma latisiliquum, Guazuma ulmifolia, Cnidoscolus aconitifolius, Gliricidia sepium and Bursera simaruba, using Cynodon plectostachyus grass as control. The results showed a wide variation in the chemical composition of the foliage, as well as in the ruminal microbiota. The crude protein (CP) content ranged from 11 to 25%, whereas the content of condensed tannins (CT) and saponins (S) was from 0.02 to 7%, and 3.2 to 6.6%, respectively. The greatest dry matter degradability (DMD) after 72 h was 69% and the least 35%, the latter coinciding with the least gas production (GP). A negative correlation was found between the CT and CH4 production, also between protozoa and fungi with the SGMT group of archaea. We concluded that the foliage of some tropical trees and shrubs has a high nutritional value and the potential to decrease CH4 production due to its CT content.

Acetylcholinesterase inhibitory activity from Amaryllis belladonna growing in Chile: enzymatic and molecular docking studies.

Alkaloid profiles from Amaryllis belladonna plants collected in Chile were examined by GC-MS to assess their inhibitory activity on acetylcholinesterase (AChE) using in vitro and in silico methodologies. The alkaloid extract was roughly separated by column chromatography on silica gel. AChE inhibitory activities from extracts and purified alkaloids were tested by the Ellman method and a molecular docking study was performed to assess the interaction between AChE and purified alkaloids. Sixteen alkaloids were found from hexane and chloroform extracts, and three were isolated and identified as buphanidrine, acetylcaranine and lycorine. Chloroform extract showed the greatest AChE inhibitory activity with IC50 value 8.89 µg/mL, whereas buphanidrine exhibited the highest inhibitory activity, with IC50 value 17.56 µg/mL. Inhibition kinetics showed that buphanidrine acts as a mixed inhibitor and molecular docking supports this inhibition mechanism. Overall, our study supports the potential use of A. belladonna as an alkaloid source with AChE inhibitory activity.[Formula: see text].

Pumpkin Waste as Livestock Feed: Impact on Nutrition and Animal Health and on Quality of Meat, Milk, and Egg.

Meat, milk, and egg contribute positively to the nutrition and health of humans; however, livestock requires a large number of resources, including land for fodder and grains. Worldwide millions of tons of vegetable waste are produced without any further processing, causing pollution and health risks. Properly managed vegetable waste could provide a source of feed for livestock, thus reducing feeding costs. In this regard, pumpkin waste (Cucurbita sp.) is an alternative. Research on pumpkin waste on animal nutrition is scarce, however, it has potential as animal feed not only for its nutritional value but also for its antioxidants, pigments, and polysaccharides content that could enhance quality of meat, milk, and egg, as well animal health. In this review, we describe the environmental impact of livestock as a result of greater demand for food of animal origin, including the importance of the consumption of animal foods in human nutrition and health. Moreover, we emphasize the potential of plant residues and, particularly, on the characteristics of pumpkins and how their use as feedstuff for livestock could improve productivity and modify the composition of meat, milk, and egg.

Considerations on the use of exogenous fibrolytic enzymes to improve forage utilization.

Digestion of cell wall fractions of forage in the rumen is incomplete due to the complex links which limit their degradation. It is therefore necessary to find options to optimize the use of forages in ruminant production systems. One alternative is to use exogenous enzymes. Exogenous fibrolytic enzymes are of fungal or bacterial origin and increase nutrient availability from the cell wall, which consists of three fractions in different proportions depending on the species of forage: digestible, potentially digestible, and indigestible. The response to addition of exogenous enzymes varies with the type of forage; many researchers infer that there are enzyme-forage interactions but fail to explain the biological mechanism. We hypothesize that the response is related to the proportion of the potentially digestible fraction. The exogenous enzyme activity depends on several factors but if the general conditions for enzyme action are available, the potentially digestible fraction may determine the magnitude of the response. Results of experiments with exogenous fibrolytic enzymes in domestic ruminants are inconsistent. This, coupled with their high cost, has made their use unattractive to farmers. Development of cheaper products exploring other microorganisms with fibrolytic activity, such as Fomes fomentarius or Cellulomonas flavigena, is required.

Effect of vitamin E on milk composition of grazing dairy cows supplemented with microencapsulated conjugated linoleic acid.

The objective of this study was to evaluate the effect of vitamin E on the fat content and fatty acid profile of grazing dairy cows supplemented with microencapsulated conjugated linoleic acid. Eight New Zealand Holstein cows in a rotational grazing system were used, in a crossover design, randomly assigned to four treatments: control (base diet with microencapsulated conjugated linoleic acid) and three levels of vitamin E (control with 4,000; 8,000; and 12,000 IU/cow per day). All the cows received a supplement apportioning 5 g of cis-9, trans-11, and 5 g of trans-10, cis-12 of conjugated linoleic acid. Moreover, they each received 4-kg dry matter (DM) concentrate and 3.2-kg DM corn silage every day. There were no differences in dry matter intake, milk production, milk composition (fat, protein, and lactose), or fatty acid profile as an effect of vitamin E, and fat content remained under 3 % in all treatments. Therefore, under the conditions that this experiment was carried out, high concentrations of vitamin E in the diet of grazing dairy cows do not inhibit milk fat depression associated with conjugated linoleic acid. It also has no effect on the fatty acid profile of the milk.

Productive response of grazing dairy cows to fresh chopped maize supplementation under a small farming system in the Mexican Highlands.

The aim of this study was to evaluate the animal performance of late lactating grazing dairy cows in response to fresh chopped maize (FCM) supplementation under a small farming system. Twenty-four multiparous Holstein dairy cows were used in a rotational grazing on a mixed alfalfa-orchard grass sward. Three treatments were evaluated: 0, 4, and 8 kg dry matter (DM) of FCM cow(-1) day(-1). The experimental design was a crossover, with three periods lasting 19 days each. There were no differences on individual milk production nor on milk composition. Herbage DM intake decreased (P < 0.0001) from 8.4 to 5.2 and 3.1 kg DM when increasing FCM from 0 to 4 and 8 kg DM, respectively. Total DM intake increased (P < 0.0001) with 8 kg DM of FCM, averaging 14.8 kg. Substitution rate was 0.75 and 0.67 kg of pasture per kilogram of FCM with 4 and 8 kg DM of FCM, respectively. Consequently, stocking rate (STR) increased (P < 0.0001) from 3.8 to 6.2 and 8.8 cows ha(-1), and milk production per hectare increased (P < 0.0001) from 39.3 to 64.5 and 95.5 kg of milk, with 0, 4, and 8 kg DM of FCM, respectively. This means that STR increases 0.63 cows ha(-1), and 0.75 kg of milk ha(-1) when FCM is increased in 1 kg of DM. In conclusion, offering FCM as supplement to grazing dairy cows in their final lactation stage had no benefits in individual milk production or milk composition, but had benefits on milk production per hectare.

Evaluación de alimentos integrales para el engorde intensivo de ovinos / Evaluation of complete feeds for intensive fattening lambs

Se realizó una evaluación de 4 alimentos comerciales para el engorde intensivo de ovinos en México, con diferentes composiciones de nutrientes. Se determinó la concentración de almidón y se estimaron las fracciones de proteína digestible en intestino (PDI). Se usaron 20 borregos machos criollos x Suffolk, con peso vivo inicial de 25,28 ± 5,7 kg en jaulas metabólicas individuales para evaluar consumo de materia seca (CMS), ganancia de peso (GDP) y conversión (CA), durante 45 días. Los alimentos (tratamientos) evaluados fueron: T1=Albapesa; T2=Purina; T3=Union Tepexpan; y T4=Hacienda. No se encontraron diferencias (P>0,05) en el CMS g/d (T1 1316; T2 1201; T3, 1124; T4 1093), GDP g/d (T1 260; T2 216; T3 285; T4 202), y CA (T1 5,05; T2 5,56; T3 3,94; T4 5,39). La estimación de la ganancia de peso basado en la digestibilidad in situ de la MS y en las ecuaciones del NRC permitieron predecir en forma adecuada el comportamiento de ovinos (r²=0,79). A pesar de que no hubo diferencias en el comportamiento, los costos de alimentación y los beneficios económicos fueron diferentes.

An evaluation of 4 commercial concentrates for intensive fattening of sheep was conducted in Mexico. Starch content was determined and protein digestible in small intestine (PDI) fractions were estimated. Twenty male sheep (criollo x Suffolk) with initial weight 25.28 ± 5.7 kg were allotted in individual crates to evaluate dry matter intake (DMI), weight gain (ADG) and feed conversion ratio (FC), during 45 days. Feed (treatments) evaluated were: T1=Albapesa; T2=Purina; T3=Union Tepexpan; and T4=Hacienda. No differences were found (P>0.05) in DMI g/d (T1 1316; T2 1201; T3, 1124; T4 1093), AGD g/d (T1 260; T2 216; T3 285; T4 202), FC (T1 5.05; T2 5.56; T3 3.94; T4 5.39). Estimation of gain based on in situ DM digestion and NRC equations allow the adequate prediction of sheep performance (r²=0.79). Despite of the lack of difference in performance, feed costs and economic benefits were different.