RESUMO
The interaction of Pt(II)(dppf)-complex, namely [Pt(dppf)(H(2)O)(2)](2+) with DNA was investigated by DPV and (1)H-NMR techniques. The results showed that the interaction process has been characterized by changes in the electrochemical parameters of both compounds and the formation of a new anodic current peak close to the anodic current peak of the [Pt(dppf)(H(2)O)(2)](2+). In addition, the (1)H-NMR spectra show that the coordination of Pt(II)(dppf)-complex to dsDNA occurs via N(7) of guanine. Others parameters like pH and ionic strength that affect the interaction process were also investigated.
Assuntos
Antineoplásicos/farmacologia , Cisplatino/farmacologia , DNA/química , Eletroquímica/métodos , Platina/química , Espectrofotometria/métodos , Antineoplásicos/química , Soluções Tampão , Cisplatino/química , Guanidina/química , Guanina/química , Concentração de Íons de Hidrogênio , Espectroscopia de Ressonância Magnética/métodos , Metais/química , Modelos Químicos , Ligação ProteicaRESUMO
In this work the interaction of DNA and the flavonoid-transition metal complex (Cu(II)-naringin complex) is characterized. The interaction was evaluated by using electrochemical ssDNA- and dsDNA-based biosensors and the results were supported by UV, CD and (1)H NMR data. In the electrochemical method, changes in the oxidation peak of the guanine and adenine bases obtained by square wave voltammetry (SWV) showed evidence of the interaction. The variations of the spectroscopic characteristics of DNA and Cu(II)-naringin complex in aqueous medium demonstrated that the predominant interaction mode may be by intercalation. Cu(II)-naringin complex interacts to dsDNA probably via N(7) of guanine site.
