RESUMO
Using voltage-clamp technique, the involvement of sigma-1 receptors in the regulation of Na+ transport in frog skin by the immunomodulatory drug glutoxim was investigated. We have shown for the first time that preincubation of the frog skin with the sigma-1 receptor antagonists haloperidol and chlorpromazine attenuates the stimulatory effect of glutoxim on the Na+ transport. The results suggest the possible involvement of the sigma-1 receptors in the regulation of Na+ transport in frog skin epithelium by glutoxim.
Assuntos
Proteínas de Anfíbios/antagonistas & inibidores , Clorpromazina/farmacologia , Haloperidol/farmacologia , Oligopeptídeos/farmacologia , Receptores sigma/antagonistas & inibidores , Pele/metabolismo , Sódio/metabolismo , Proteínas de Anfíbios/metabolismo , Animais , Transporte de Íons/efeitos dos fármacos , Rana temporaria , Receptores sigma/metabolismo , Receptor Sigma-1RESUMO
Using voltage-clamp technique, the involvement of epoxygenases in immunomodulatory drug glutoxim regulation of Na+ transport in frog skin was investigated. We have shown for the first time that preincubation of the frog skin with epoxygenase inhibitors econazole or proadifen almost completely inhibits the stimulatory effect of glutoxim on Na+ transport. The data suggest the involvement of the enzymes and/or products of epoxygenase oxidation pathway of arachidonic acid metabolism in glutoxim effect on Na+ transport in frog skin epithelium.
Assuntos
Econazol/farmacologia , Inibidores Enzimáticos/farmacologia , Oligopeptídeos , Oxirredutases/antagonistas & inibidores , Pele/metabolismo , Sódio/metabolismo , Animais , Transporte de Íons/efeitos dos fármacos , Oligopeptídeos/farmacocinética , Oligopeptídeos/farmacologia , Rana temporariaRESUMO
Using voltage-clamp technique, the involvement of lipoxygenases in the effect of immunomodulatory drug glutoxim on Na+ transport in frog skin was investigated. It was shown for the first time that preincubation of the skin with lipoxygenase inhibitors caffeic acid, baicalein, and nordihydroguaiaretic acid significantly decreases the stimulatory effect of glutoxim on Na+ transport. The data suggest the involvement of lipoxygenase oxidation pathway of arachidonic acid metabolism in the glutoxim effect on Na+ transport in frog skin epithelium.
Assuntos
Lipoxigenases/metabolismo , Oligopeptídeos/farmacologia , Pele/efeitos dos fármacos , Pele/metabolismo , Sódio/metabolismo , Animais , Anuros , Transporte Biológico/efeitos dos fármacos , Epitélio/efeitos dos fármacos , Epitélio/metabolismo , CinéticaRESUMO
Using voltage-clamp technique, the involvement of WASP proteins and Arp2/3 complex in the effect of immunomodulator drug glutoxim on Na(+) transport in frog skin was investigated. It was shown for the first time that preincubation of the skin with the N-WASP inhibitor wiskostatin or the Arp2/3 complex inhibitor CK-0944666 significantly decreases the stimulatory effect of glutoxim on Na(+) transport. The data suggest the involvement of actin filament polymerization and branching in the glutoxim effect on Na(+) transport in frog skin.
