Visceral leishmaniasis and HIV coinfection in Brazil: epidemiological profile and spatial patterns.

BACKGROUND: Geographical overlap of human visceral leishmaniasis (HVL) and HIV infection favors occurrences of HVL/HIV coinfection. The increasing numbers of cases of HVL/HIV coinfection are a worldwide concern and knowledge of the factors involved can help in developing preventive measures. METHODS: We aimed to identify spatial patterns and describe the epidemiological profile of HVL/HIV coinfection in Brazil from 2007 to 2015. This was an ecological study, in which thematic maps were created through geoprocessing tools, based on secondary data obtained from open-access platforms, to identify priority areas for interventions for controlling HLV/HIV coinfection, using the TerraView 4.2.2 software. RESULTS: We found spatial autocorrelations between the HVL/HIV rates of neighboring municipalities according to the global Moran index (0.10; p<0.01). The highest HVL/HIV rate was in the central-western region. Among the epidemiological characteristics according to the regions, an increasing trend in the annual variation rate was observed, with positive values over the years and statistical significance (p<0.05) in the North with 1.62 (95% CI 0.57 to 2.69; p=0.02) and Northeast with 6.41 (95% CI 2.60 to 10.37; p=0.006). Similarly, increasing trends were observed in the states of Maranhão with 21.34 (95% CI 13.99 to 9.16; p<0.001) and Sergipe with 5.44 (95% CI 0.61 to 10.50; p=0.04). CONCLUSIONS: Our results showed spatial overlap between occurrences of HVL and HIV with spatial patterns of HVL/HIV coinfection, thus revealing that the numbers of cases reported are increasing. Identifying areas with higher coinfection indices contributes to applying interventions and control measures among targeted populations, to prevent new cases.

Situational profile of equidae breeders in the municipality of Soure, Pará: an approach to glanders and adoption of relevant sanitary measures / Perfil situacional dos criadores de equídeos do município de Soure, Pará: uma abordagem quanto ao mormo e a adoção das medidas sanitárias pertinentes

A survey was conducted on the knowledge, attitudes, and behaviors of Equidae breeders in the municipality of Soure, Marajó Island, Pará, regarding animal health problems in the properties, especially glanders and adoption of sanitary measures that are pertinent to their combat, to elaborate on educational technical material with the appropriate methodology. The study included 50 interviewees from urban and rural areas of this municipality. Regarding data collection, structured interviews containing 22 multiple choice questions on the socioeconomic characteristics of breeders and/or owners of Equidae, breeding habits and models, and conceptions about sanitary control and their relationship with the animal health defense agency were conducted. From the qualitative analysis of data, 66% (33/50) interviewees were identified as literate, which facilitates the choice of the best language to use for communication. Regarding the choice of the best language for the transfer of information, 62% (31/50) interviewees said that a mobile phone is currently the best tool for transmitting information. Further, 40% (20/50) interviewees breed their Equidae extensively, raising an alert for epidemiological surveillance, and only 10% (5/50) interviewees turned to ADEPARÁ when the animals were sick. Based on the study results, Equidae breeders need guidance regarding the actions of the animal health defense services, and sanitary education is a fundamental measure for raising awareness and clarification of glanders to mitigate damage to public health and animal welfare, avoiding its spread in the State.(AU)

Foi realizado um levantamento sobre conhecimentos, atitudes e comportamentos dos criadores de equídeos do município de Soure, na Ilha do Marajó, Pará, quanto aos problemas sanitários nas propriedades, em especial, ao mormo e a adoção de medidas sanitárias pertinentes ao seu combate - elaboração de material técnico educativo com metodologia ade-quada ao público-alvo trabalhado. A pesquisa contou com 50 entrevistados pertencentes às zonas urbana e rural desse muni-cípio. Quanto a coleta de dados, foram realizadas entrevistas estruturadas contendo 22 questões de múltipla escolha referentes às características socioeconômicas dos criadores e/ou proprietários de equídeos; aos hábitos e aos modos de criação; às con-cepções sobre o controle sanitário e a relação destes com o órgão de defesa sanitária animal. Da análise qualitativa dos dados, observou-se que 66% (33/50) dos entrevistados são alfabetizados; o que facilita a escolha da melhor linguagem para o repasse de informações; 62% (31/50) elencou que o celular, atualmente, é a melhor ferramenta para a transmissão de informações; 40% (20/50) cria seus equídeos de maneira extensiva, representando um alerta à vigilância epidemiológica e apenas 10% (5/50) recorre à ADEPARÁ quando os animais adoecem. Com base no estudo, foi possível concluir que os criadores de equídeos necessitam de orientações quanto às ações do serviço de defesa sanitária animal, sendo a educação sanitária medida fundamen-tal para conscientização e esclarecimento deles sobre o mormo, de maneira a mitigar seus danos à saúde pública e ao bem-estar dos animais, evitando sua propagação no Estado.(AU)

Evaluation of teaser buffaloes subjected to surgical technique for fixation of the caudal curvature of the sigmoid flexure with myectomy of the penis retractor muscle / Avaliação de rufiões bubalinos submetidos à técnica cirúrgica de fixação da curvatura caudal da flexura sigmóide com miectomia do músculo retrator do pênis

The objective of this work was to evaluate the efficiency technique of fixation of the caudal curvature of the sigmoid flexure with myectomy of the penis retractor muscle and the performance of teaser bulls after surgery, through the detection of females in estrus, without performing the copulation. 12 teaser buffaloes were used without defined breed, with an average age of 20 months, weighing on average 200 kg. The animals were subjected to food and water fasting for 12 hours to provide tranquilization in advance. The surgical procedure consisted of the exteriorization of the sigmoid flexure, myectomy of  penis retractor muscle and fixation of the caudal curvature of the penis sigmoid flexure. Of the 12 evaluated teaser buffaloes in the libido test, 100% performed  the  flehmen reflex, mount attempt, incomplete and complete jump  with total inability of  the penis exposure.    It was concluded that this technique can be used safely, having  simplicity in its execution, effective results and mainly low cost, not interfering in the animals libido. The animals when tested as teaser buffaloes kept the libido during the experimental period with total inability of exposure of the penis.(AU)

O objetivo do presente trabalho foi avaliar a técnica de fixação da curvatura caudal da flexura sigmóide com miectomia do músculo retratordo pênis e o desempenho de rufiões bubalinos após cirurgia, através da detecção de fêmeas em estro, sem executar a cópula. Foram utilizados 12 bubalinos sem raça definida, com idade média de 20 meses, pesando em média 200 kg. Os animais foram submetidos ajejum alimentar e hídrico de 12 horas para tranquilização prévia. O procedimento cirúrgico constou naexteriorização da flexura sigmóide, miectomia do músculo retrator do pênis e fixação da curvatura caudal da flexura sigmóide do pênis. Dos 12 rufiões avaliados no teste de libido, 100% realizaram reflexode flehmen, tentativa de monta, salto incompleto e completo com total incapacidade de exposição do pênis. Conclui-se que esta técnica pode ser utilizada com segurança, apresentando simplicidade na sua execução, eficácia nos resultados e principalmente baixo custo, não interferindo na libidodos animais. Os animais quando testados como rufiões mantiveram a libido durante o período experimental com total incapacidade de exposição do pênis.(AU)

Evaluation of teaser buffaloes subjected to surgical technique for fixation of the caudal curvature of the sigmoid flexure with myectomy of the penis retractor muscle / Avaliação de rufiões bubalinos submetidos à técnica cirúrgica de fixação da curvatura caudal da flexura sigmóide com miectomia do músculo retrator do pênis

The objective of this work was to evaluate the efficiency technique of fixation of the caudal curvature of the sigmoid flexure with myectomy of the penis retractor muscle and the performance of teaser bulls after surgery, through the detection of females in estrus, without performing the copulation. 12 teaser buffaloes were used without defined breed, with an average age of 20 months, weighing on average 200 kg. The animals were subjected to food and water fasting for 12 hours to provide tranquilization in advance. The surgical procedure consisted of the exteriorization of the sigmoid flexure, myectomy of  penis retractor muscle and fixation of the caudal curvature of the penis sigmoid flexure. Of the 12 evaluated teaser buffaloes in the libido test, 100% performed  the  flehmen reflex, mount attempt, incomplete and complete jump  with total inability of  the penis exposure.    It was concluded that this technique can be used safely, having  simplicity in its execution, effective results and mainly low cost, not interfering in the animals libido. The animals when tested as teaser buffaloes kept the libido during the experimental period with total inability of exposure of the penis.

O objetivo do presente trabalho foi avaliar a técnica de fixação da curvatura caudal da flexura sigmóide com miectomia do músculo retratordo pênis e o desempenho de rufiões bubalinos após cirurgia, através da detecção de fêmeas em estro, sem executar a cópula. Foram utilizados 12 bubalinos sem raça definida, com idade média de 20 meses, pesando em média 200 kg. Os animais foram submetidos ajejum alimentar e hídrico de 12 horas para tranquilização prévia. O procedimento cirúrgico constou naexteriorização da flexura sigmóide, miectomia do músculo retrator do pênis e fixação da curvatura caudal da flexura sigmóide do pênis. Dos 12 rufiões avaliados no teste de libido, 100% realizaram reflexode flehmen, tentativa de monta, salto incompleto e completo com total incapacidade de exposição do pênis. Conclui-se que esta técnica pode ser utilizada com segurança, apresentando simplicidade na sua execução, eficácia nos resultados e principalmente baixo custo, não interferindo na libidodos animais. Os animais quando testados como rufiões mantiveram a libido durante o período experimental com total incapacidade de exposição do pênis.

Parasitological and histological aspects of Holochilus sciureus naturally infected by Schistosoma mansoni / Aspectos parasitológicos e histológicos de Holochilus sciureus naturalmente infectados por Schistosoma mansoni

Abstract Schistosomiasis is a neglected disease that affects millions of people around the world, being common in the state of Maranhão. A total of 225 rodents of the Holochilus sciureus species from the Western Lowland Maranhão were studied, of which 144 animals (64%) exhibited Schistosoma eggs in their feces samples. Macroscopic lesions characterized as well-defined whitish areas on the liver and spleen surfaces were observed. Histopathological examination revealed multifocal granulomas in the esophagus, liver, spleen, pancreas and duodenum, with structures compatible with Schistosoma mansoni eggs, as well as severe hepatic micro-vacuolar degeneration, multifocal and coalescent, with proliferation of random bile ducts and associated epithelial hyperplasia to areas of fibrosis. Adult forms of the parasite were observed in the blood vessels of the portal space. The lungs exhibited moderate and diffuse interstitial pneumonia with intralesional S. mansoni eggs. In the kidneys, hyaline cylinders were observed in the pelvis and diffuse hemorrhage. In conclusion, H. sciureus displays a pathological picture similar to human being. This rodent plays a role as sentinel in Baixada Maranhense.

Resumo A esquistossomose é uma doença negligenciada que afeta milhões de pessoas em todo o mundo, sendo comum no estado do Maranhão. Um total de 225 roedores da espécie Holochilus sciureus da Planície Ocidental do Maranhão foram estudados, dos quais 144 animais (64%) apresentaram ovos de Schistosoma em suas fezes. Lesões macroscópicas caracterizadas como áreas esbranquiçadas bem definidas nas superfícies do fígado e baço foram observadas. O exame histopatológico revelou granulomas multifocais no esôfago, fígado, baço, pâncreas e duodeno, com estruturas compatíveis com ovos de Schistosoma mansoni, degeneração micro-vacuolar hepática grave, multifocal e coalescente, com proliferação de ductos biliares aleatórios e hiperplasia epitelial associada a áreas de fibrose. Formas adultas do parasito foram observadas nos vasos sanguíneos do espaço portal. Os pulmões exibiram pneumonia intersticial moderada e difusa com ovos de S. mansoni intralesionais. Nos rins, foram observados cilindros hialinos na pelve e hemorragia difusa. Em conclusão, H. sciureus apresenta um quadro patológico semelhante ao ser humano. Este roedor desempenha um papel de sentinela na Baixada Maranhense.

Parasitological and histological aspects of Holochilus sciureus naturally infected by Schistosoma mansoni.

Schistosomiasis is a neglected disease that affects millions of people around the world, being common in the state of Maranhão. A total of 225 rodents of the Holochilus sciureus species from the Western Lowland Maranhão were studied, of which 144 animals (64%) exhibited Schistosoma eggs in their feces samples. Macroscopic lesions characterized as well-defined whitish areas on the liver and spleen surfaces were observed. Histopathological examination revealed multifocal granulomas in the esophagus, liver, spleen, pancreas and duodenum, with structures compatible with Schistosoma mansoni eggs, as well as severe hepatic micro-vacuolar degeneration, multifocal and coalescent, with proliferation of random bile ducts and associated epithelial hyperplasia to areas of fibrosis. Adult forms of the parasite were observed in the blood vessels of the portal space. The lungs exhibited moderate and diffuse interstitial pneumonia with intralesional S. mansoni eggs. In the kidneys, hyaline cylinders were observed in the pelvis and diffuse hemorrhage. In conclusion, H. sciureus displays a pathological picture similar to human being. This rodent plays a role as sentinel in Baixada Maranhense.

Parasitological and histological aspects of Holochilus sciureus naturally infected by Schistosoma mansoni / Aspectos parasitológicos e histológicos de Holochilus sciureus naturalmente infectados por Schistosoma mansoni

Schistosomiasis is a neglected disease that affects millions of people around the world, being common in the state of Maranhão. A total of 225 rodents of the Holochilus sciureus species from the Western Lowland Maranhão were studied, of which 144 animals (64%) exhibited Schistosoma eggs in their feces samples. Macroscopic lesions characterized as well-defined whitish areas on the liver and spleen surfaces were observed. Histopathological examination revealed multifocal granulomas in the esophagus, liver, spleen, pancreas and duodenum, with structures compatible with Schistosoma mansoni eggs, as well as severe hepatic micro-vacuolar degeneration, multifocal and coalescent, with proliferation of random bile ducts and associated epithelial hyperplasia to areas of fibrosis. Adult forms of the parasite were observed in the blood vessels of the portal space. The lungs exhibited moderate and diffuse interstitial pneumonia with intralesional S. mansoni eggs. In the kidneys, hyaline cylinders were observed in the pelvis and diffuse hemorrhage. In conclusion, H. sciureus displays a pathological picture similar to human being. This rodent plays a role as sentinel in Baixada Maranhense.(AU)

A esquistossomose é uma doença negligenciada que afeta milhões de pessoas em todo o mundo, sendo comum no estado do Maranhão. Um total de 225 roedores da espécie Holochilus sciureus da Planície Ocidental do Maranhão foram estudados, dos quais 144 animais (64%) apresentaram ovos de Schistosoma em suas fezes. Lesões macroscópicas caracterizadas como áreas esbranquiçadas bem definidas nas superfícies do fígado e baço foram observadas. O exame histopatológico revelou granulomas multifocais no esôfago, fígado, baço, pâncreas e duodeno, com estruturas compatíveis com ovos de Schistosoma mansoni, degeneração micro-vacuolar hepática grave, multifocal e coalescente, com proliferação de ductos biliares aleatórios e hiperplasia epitelial associada a áreas de fibrose. Formas adultas do parasito foram observadas nos vasos sanguíneos do espaço portal. Os pulmões exibiram pneumonia intersticial moderada e difusa com ovos de S. mansoni intralesionais. Nos rins, foram observados cilindros hialinos na pelve e hemorragia difusa. Em conclusão, H. sciureus apresenta um quadro patológico semelhante ao ser humano. Este roedor desempenha um papel de sentinela na Baixada Maranhense.(AU)

Histopathological findings and immunohistochemical staining of Neospora caninum infection in placentas of animals for slaughter / Achados histopatológicos e marcação imuno-histoquímica da infecção por Neospora caninum em placentas de animais destinados ao abate

Bovine neosporosis is a parasitic disease caused by the protozoan Neospora caninum, which is one of the main pathogens that cause abortions in cattle worldwide. It is responsible for large losses in dairy and beef cattle, especially with regard to the expenses associated with decreased productivity and increased discard. In the State of Maranhão, the occurrence of this parasite has already been confirmed in the dairy cattle of three microregions that make up the state's dairy industry and in small ruminants of the municipalities of Amarante do Maranhão and Buritirana. This fact justifies the importance of determining the presence of N. caninum in cattle slaughtered in the slaughterhouses of the Island of São Luís, to help control the parasitic cycle in the cattle production system of the region under study, and the importance of its diagnosis. The objective of the research was to conduct a histopathological and immunohistochemical characterization of bovine placentas infected with N. caninum collected in slaughterhouses. Sixteen placenta samples from cows were randomly selected at the slaughterhouses of the city of São Luís for histological analysis and immunohistochemistry. Results showed that 31% of the placentas presented histopathological changes such as: inflammatory process, edema, hemorrhage and presence of hemosiderin. All slides with histopathological changes showed immunolabeling for N. caninum.(AU)

A neosporose bovina é uma doença parasitária causada pelo protozoário Neospora caninum, que é um dos principais patógenos causadores de aborto em bovinos no mundo. É responsável por grandes prejuízos nos rebanhos de leite e de corte, principalmente no que se refere aos gastos associados à diminuição da produtividade e aumento do descarte. No estado do Maranhão, a ocorrência deste parasito já foi confirmada em rebanhos de bovinos de leite de três microrregiões que compõem a bacia leiteira do estado e em pequenos ruminantes dos municípios de Amarante do Maranhão e Buritirana. Justificando desta forma a importância de determinar a presença de N. caninum nos bovinos abatidos nos matadouros da Ilha de São Luís, para auxiliar no controle do ciclo parasitário no sistema de produção de bovinos na região em estudo e a importância do seu diagnóstico. O objetivo da pesquisa foi realizar a caracterização histopatológica e imuno-histoquímica de placentas bovinas infectadas por N. caninum coletadas em matadouro. Foram selecionadas ao acaso 16 amostras de placenta de vacas, provenientes de abatedouros do município de São Luís para análise histológica e imuno-histoquímica. Os resultados evidenciaram que 31% das placentas apresentaram alterações histopatológicas, tais como: processo inflamatório, edema, hemorragia e presença de hemossiderina. Todas as lâminas com alterações histopatológicas apresentaram imunomarcação para N. caninum.(AU)

Bone alkaline phosphatase in donkeys obtained by the thermal inactivation method

The bone alkaline phosphatase has clinical importance in pathological orthopedic conditions as a marker of the osteoblastic activity, especially in malignant bone tumors and therapeutic monitoring of osteoporosis and other diseases. The reaction of Roy quantitative and qualitative technique of thermal inactivation was applied to obtain bone alkaline phosphatase isoenzyme of male and female donkeys, without defined breed (SRD) aged from seven to 14 months, subjected to the same diet, divided into two groups, composed of five animals each, being the group 1 (G1) kept in confinement and the Group 2 (G2) in traction activity. Blood samples were collected in eight times with an interval of 90 days, during 21 months. The percentage of thermostable isoenzyme was 35 for the G1 and 32 for the G2. It was concluded that the measurements of bone alkaline phosphatase by the thermal inactivation method showed no changes when compared among the groups, demonstrating that the activity of animal traction exerted by the donkeys do not interfere in bone metabolism of these animals.(AU)

Bone alkaline phosphatase in donkeys obtained by the thermal inactivation method

The bone alkaline phosphatase has clinical importance in pathological orthopedic conditions as a marker of the osteoblastic activity, especially in malignant bone tumors and therapeutic monitoring of osteoporosis and other diseases. The reaction of Roy quantitative and qualitative technique of thermal inactivation was applied to obtain bone alkaline phosphatase isoenzyme of male and female donkeys, without defined breed (SRD) aged from seven to 14 months, subjected to the same diet, divided into two groups, composed of five animals each, being the group 1 (G1) kept in confinement and the Group 2 (G2) in traction activity. Blood samples were collected in eight times with an interval of 90 days, during 21 months. The percentage of thermostable isoenzyme was 35 for the G1 and 32 for the G2. It was concluded that the measurements of bone alkaline phosphatase by the thermal inactivation method showed no changes when compared among the groups, demonstrating that the activity of animal traction exerted by the donkeys do not interfere in bone metabolism of these animals.

Histopathological findings and immunohistochemical staining of Neospora caninum infection in placentas of animals for slaughter / Achados histopatológicos e marcação imuno-histoquímica da infecção por Neospora caninum em placentas de animais destinados ao abate

Bovine neosporosis is a parasitic disease caused by the protozoan Neospora caninum, which is one of the main pathogens that cause abortions in cattle worldwide. It is responsible for large losses in dairy and beef cattle, especially with regard to the expenses associated with decreased productivity and increased discard. In the State of Maranhão, the occurrence of this parasite has already been confirmed in the dairy cattle of three microregions that make up the state's dairy industry and in small ruminants of the municipalities of Amarante do Maranhão and Buritirana. This fact justifies the importance of determining the presence of N. caninum in cattle slaughtered in the slaughterhouses of the Island of São Luís, to help control the parasitic cycle in the cattle production system of the region under study, and the importance of its diagnosis. The objective of the research was to conduct a histopathological and immunohistochemical characterization of bovine placentas infected with N. caninum collected in slaughterhouses. Sixteen placenta samples from cows were randomly selected at the slaughterhouses of the city of São Luís for histological analysis and immunohistochemistry. Results showed that 31% of the placentas presented histopathological changes such as: inflammatory process, edema, hemorrhage and presence of hemosiderin. All slides with histopathological changes showed immunolabeling for N. caninum.

A neosporose bovina é uma doença parasitária causada pelo protozoário Neospora caninum, que é um dos principais patógenos causadores de aborto em bovinos no mundo. É responsável por grandes prejuízos nos rebanhos de leite e de corte, principalmente no que se refere aos gastos associados à diminuição da produtividade e aumento do descarte. No estado do Maranhão, a ocorrência deste parasito já foi confirmada em rebanhos de bovinos de leite de três microrregiões que compõem a bacia leiteira do estado e em pequenos ruminantes dos municípios de Amarante do Maranhão e Buritirana. Justificando desta forma a importância de determinar a presença de N. caninum nos bovinos abatidos nos matadouros da Ilha de São Luís, para auxiliar no controle do ciclo parasitário no sistema de produção de bovinos na região em estudo e a importância do seu diagnóstico. O objetivo da pesquisa foi realizar a caracterização histopatológica e imuno-histoquímica de placentas bovinas infectadas por N. caninum coletadas em matadouro. Foram selecionadas ao acaso 16 amostras de placenta de vacas, provenientes de abatedouros do município de São Luís para análise histológica e imuno-histoquímica. Os resultados evidenciaram que 31% das placentas apresentaram alterações histopatológicas, tais como: processo inflamatório, edema, hemorragia e presença de hemossiderina. Todas as lâminas com alterações histopatológicas apresentaram imunomarcação para N. caninum.

Productive characteristics of meat quails reared in different environments / Características produtivas de codornas de corte mantidas em diferentes ambientes

The objective of this study was to evaluate the effects of ambient temperature and consumption on the performance, carcass yields and cuts, organs, and body temperatures of meat quails. The experiment was conducted at the Federal University of Maranhão, campus of Chapadinha-MA, during September to October 2013. From the 14th day of age, 450 meat quails, not sexed, with an average initial weight of 90.4 ± 12.9 g, were distributed in batteries and accommodated in either acclimatized or non-acclimatized rooms, until 42 days of age. The experimental design was completely randomized, with three treatments, and six replicates of 25 birds each. The treatments were CE (acclimatized environment at 26 °C); NE (natural environment), and CECC (acclimatized environment at 26 °C with controlled feed, to keep the same consumption level as that of the birds in NE). The evaluated variables at 28 and 42 days of age were as follows: (1) the intake of feed, weight gain, feed conversion, body weight, and energy efficiency to weight gain, (2) the carcass weight, carcass yields (%) for breast, thigh, drumstick, and wing, and (3) the relative weights (%) of the digestive tract, heart, liver, gizzard, and intestine. In addition, we evaluated the cloacal and surface temperatures (°C), and subsequently, calculated the average and surface body temperatures. The treatment means were compared using the Student-Newman-Keuls test, with 5% level of significance. Compared with the acclimatized ambient environment, the natural environment more favored the performance of quails up to 28 days, but then affected it negatively from that age onward. Carcass yield, organs, and internal temperature of the quails maintained in the acclimatized and natural environments were not influenced.(AU)

Objetivou-se avaliar os efeitos da temperatura ambiente sobre o desempenho, rendimentos de carcaça e cortes, órgãos e temperaturas corporais de codornas de corte. O experimento foi conduzido na Universidade Federal do Maranhão, campus de Chapadinha-MA, no período de setembro a outubro de 2014. A partir do 14º dia de idade, 450 codornas de corte, não sexadas, com o peso inicial médio de 90,4 ± 12,9g, foram distribuídas em baterias, acondicionadas em sala climatizada e não climatizada, até o 42° dia. O delineamento experimental foi o inteiramente casualizado, com três tratamentos e seis repetições de 25 aves. Os tratamentos consistiram em: AC (Ambiente climatizado com temperatura de 26°C); AN (Ambiente natural) e ACCC (Ambiente climatizado com temperatura de 26°C com alimentação controlada para manter o mesmo consumo das aves mantidas no AN). Foi avaliado o consumo de ração, ganho de peso, conversão alimentar, peso vivo, eficiência energética para ganho de peso; o peso de carcaça, rendimentos (%) de carcaça, peito, coxa, sobrecoxa e asa; os pesos relativos (%) do trato digestório, coração, fígado, moela e intestino aos 28 e aos 42 dias. Também foram avaliadas as temperaturas (°C) cloacais e superficiais, e posteriormente, calculadas a superficial média e corporal média. As médias dos tratamentos foram comparadas pelo teste SNK considerando o nível de 5% de significância. O ambiente natural melhorou o desempenho das codornas até os 28 dias, prejudicando a partir dessa idade em relação ao ambiente climatizado. Os rendimentos de carcaça, órgãos e a temperatura interna das codornas mantidas nos ambientes climatizado e natural, não foram influenciados.(AU)

Productive characteristics of meat quails reared in different environments / Características produtivas de codornas de corte mantidas em diferentes ambientes

The objective of this study was to evaluate the effects of ambient temperature and consumption on the performance, carcass yields and cuts, organs, and body temperatures of meat quails. The experiment was conducted at the Federal University of Maranhão, campus of Chapadinha-MA, during September to October 2013. From the 14th day of age, 450 meat quails, not sexed, with an average initial weight of 90.4 ± 12.9 g, were distributed in batteries and accommodated in either acclimatized or non-acclimatized rooms, until 42 days of age. The experimental design was completely randomized, with three treatments, and six replicates of 25 birds each. The treatments were CE (acclimatized environment at 26 °C); NE (natural environment), and CECC (acclimatized environment at 26 °C with controlled feed, to keep the same consumption level as that of the birds in NE). The evaluated variables at 28 and 42 days of age were as follows: (1) the intake of feed, weight gain, feed conversion, body weight, and energy efficiency to weight gain, (2) the carcass weight, carcass yields (%) for breast, thigh, drumstick, and wing, and (3) the relative weights (%) of the digestive tract, heart, liver, gizzard, and intestine. In addition, we evaluated the cloacal and surface temperatures (°C), and subsequently, calculated the average and surface body temperatures. The treatment means were compared using the Student-Newman-Keuls test, with 5% level of significance. Compared with the acclimatized ambient environment, the natural environment more favored the performance of quails up to 28 days, but then affected it negatively from that age onward. Carcass yield, organs, and internal temperature of the quails maintained in the acclimatized and natural environments were not influenced.

Objetivou-se avaliar os efeitos da temperatura ambiente sobre o desempenho, rendimentos de carcaça e cortes, órgãos e temperaturas corporais de codornas de corte. O experimento foi conduzido na Universidade Federal do Maranhão, campus de Chapadinha-MA, no período de setembro a outubro de 2014. A partir do 14º dia de idade, 450 codornas de corte, não sexadas, com o peso inicial médio de 90,4 ± 12,9g, foram distribuídas em baterias, acondicionadas em sala climatizada e não climatizada, até o 42° dia. O delineamento experimental foi o inteiramente casualizado, com três tratamentos e seis repetições de 25 aves. Os tratamentos consistiram em: AC (Ambiente climatizado com temperatura de 26°C); AN (Ambiente natural) e ACCC (Ambiente climatizado com temperatura de 26°C com alimentação controlada para manter o mesmo consumo das aves mantidas no AN). Foi avaliado o consumo de ração, ganho de peso, conversão alimentar, peso vivo, eficiência energética para ganho de peso; o peso de carcaça, rendimentos (%) de carcaça, peito, coxa, sobrecoxa e asa; os pesos relativos (%) do trato digestório, coração, fígado, moela e intestino aos 28 e aos 42 dias. Também foram avaliadas as temperaturas (°C) cloacais e superficiais, e posteriormente, calculadas a superficial média e corporal média. As médias dos tratamentos foram comparadas pelo teste SNK considerando o nível de 5% de significância. O ambiente natural melhorou o desempenho das codornas até os 28 dias, prejudicando a partir dessa idade em relação ao ambiente climatizado. Os rendimentos de carcaça, órgãos e a temperatura interna das codornas mantidas nos ambientes climatizado e natural, não foram influenciados.

Leishmania (Leishmania) amazonensis: experimental cutaneous leishmaniasis associated with systemic amyloidosis in mice.

We infected Swiss and C57BL/6 female mice in the left hind footpad with 10(4)Leishmania (L.) amazonensis promastigotes in stationary phase. The macroscopic examination showed a nodular non-ulcerated lesion at the site of inoculation and hepatic and spleenic enlargement. Histopathologically, the primary lesion showed an extensive liquefactive necrosis and inflammatory infiltrate, mainly consisting of macrophages filled with amastigotes, and rare lymphocytes. The inflammatory reaction in liver, spleen and kidney showed amyloid deposits. Additionally, C57BL/6 had accentuated amyloidosis in both ovarian cortical and medullar region and inflammatory infiltrates in the pancreas and adrenal gland.