Use of glucose or BTS™ combined with DMSO or methylglycol under two differentfreezing protocols for the cryopreservation of sperm from the common curimatã(Prochilodus brevis)

This study evaluated the effect of glucose orBeltsville Thawing Solution (BTS™) combined withdimethyl sulfoxide (DMSO) or methylglycol (MG)under two different freezing protocols on the kineticsand morphology of cryopreserved Prochilodus brevissperm. The semen samples were diluted using one of fourdifferent treatments (glucose+DMSO, glucose+MG,BTS™+DMSO, and BTS™+MG), loaded into 0.25-mlstraws and subjected to two different freezing processes(programmed freezing machine and dry shipper). After10 days, the semen samples were thawed, and the spermmorphology and kinetics were evaluated. Thephysicochemical parameters of the semen in naturawere similar to those observed in other studies ofCharaciformes, indicating the feasibility of semencryopreservation. Glucose, when used as a diluentwith the cryoprotectant MG (glucose+MG), yieldedhigher percentages of mobile spermatozoa afterfreezing in a dry shipper (76.88 ± 4.84%) and in aprogrammed freezing machine (70.95 ± 1.76%)compared with the combination of glucose and DMSO.Moreover, the glucose+MG treatment yielded a highersperm velocity (curvilinear velocity: 79.52 ± 2.88 µm s-1; straight-line velocity: 45.46 ± 3.01 µm s-1; averagepath velocity: 67.92 ± 3.08 µm s-1) than the otherstudied treatments, and a higher amount of normalsperm (74.56 ± 0.77%) was observed in the semensamples cryopreserved using a programmed freezingmachine. The sperm abnormalities observed included abent tail morphology. Therefore, the use ofglucose+MG in combination with either a dry shipper ora programmed freezing machine is recommended forthe cryopreservation of P. brevis sperm because thesemethods yielded high numbers of motile andmorphologically normal spermatozoa.

Use of glucose or BTS™ combined with DMSO or methylglycol under two differentfreezing protocols for the cryopreservation of sperm from the common curimatã(Prochilodus brevis)

This study evaluated the effect of glucose orBeltsville Thawing Solution (BTS™) combined withdimethyl sulfoxide (DMSO) or methylglycol (MG)under two different freezing protocols on the kineticsand morphology of cryopreserved Prochilodus brevissperm. The semen samples were diluted using one of fourdifferent treatments (glucose+DMSO, glucose+MG,BTS™+DMSO, and BTS™+MG), loaded into 0.25-mlstraws and subjected to two different freezing processes(programmed freezing machine and dry shipper). After10 days, the semen samples were thawed, and the spermmorphology and kinetics were evaluated. Thephysicochemical parameters of the semen in naturawere similar to those observed in other studies ofCharaciformes, indicating the feasibility of semencryopreservation. Glucose, when used as a diluentwith the cryoprotectant MG (glucose+MG), yieldedhigher percentages of mobile spermatozoa afterfreezing in a dry shipper (76.88 ± 4.84%) and in aprogrammed freezing machine (70.95 ± 1.76%)compared with the combination of glucose and DMSO.Moreover, the glucose+MG treatment yielded a highersperm velocity (curvilinear velocity: 79.52 ± 2.88 µm s-1; straight-line velocity: 45.46 ± 3.01 µm s-1; averagepath velocity: 67.92 ± 3.08 µm s-1) than the otherstudied treatments, and a higher amount of normalsperm (74.56 ± 0.77%) was observed in the semensamples cryopreserved using a programmed freezingmachine. The sperm abnormalities observed included abent tail morphology. Therefore, the use ofglucose+MG in combination with either a dry shipper ora programmed freezing machine is recommended forthe cryopreservation of P. brevis sperm because thesemethods yielded high numbers of motile andmorphologically normal spermatozoa.(AU)

Aloe vera na criopreservação do sêmen de tambaqui (Colossoma macropomum) / Aloe vera in the cryopreservation of tambaqui (Colossoma macropomum) sperm

This study aimed to evaluate the extract of Aloe vera (AV) associated or not with 10% Dimethylsulfoxide (DMSO) in cryopreservation of tambaqui semen. For the formation of the pools (n= 14), 30 males were hormonally induced twice. Each pool had the objective motility, curvilinear velocity, straight-line velocity, average path velocity and morphology analyzed before and after cryopreservation of semen. The means for cryopreservation were constituted of Powder Coconut Water-104 diluent added DMSO and/or AV (5 or 10%). After cryopreservation, motility, velocities and morphology were reduced significantly when compared to fresh semen. For sperm motility the best treatment was that using only DMSO (20,86±8,31) and DMSO + 5% AV (15.71±9.77). For the velocities, the worse treatment was DMSO+10% AV. Treatment with only the addition of DMSO had a significantly higher effect than others on percentage of morphologically normal sperm. The mean correlation found was between motilityand the rate of morphologically normal sperm (r = 0.687). In conclusion, the addition of AV does not provide greater protection for spermatozoa during cryopreservation.

Aloe vera na criopreservação do sêmen de tambaqui (Colossoma macropomum) / Aloe vera in the cryopreservation of tambaqui (Colossoma macropomum) sperm

This study aimed to evaluate the extract of Aloe vera (AV) associated or not with 10% Dimethylsulfoxide (DMSO) in cryopreservation of tambaqui semen. For the formation of the pools (n= 14), 30 males were hormonally induced twice. Each pool had the objective motility, curvilinear velocity, straight-line velocity, average path velocity and morphology analyzed before and after cryopreservation of semen. The means for cryopreservation were constituted of Powder Coconut Water-104 diluent added DMSO and/or AV (5 or 10%). After cryopreservation, motility, velocities and morphology were reduced significantly when compared to fresh semen. For sperm motility the best treatment was that using only DMSO (20,86±8,31) and DMSO + 5% AV (15.71±9.77). For the velocities, the worse treatment was DMSO+10% AV. Treatment with only the addition of DMSO had a significantly higher effect than others on percentage of morphologically normal sperm. The mean correlation found was between motilityand the rate of morphologically normal sperm (r = 0.687). In conclusion, the addition of AV does not provide greater protection for spermatozoa during cryopreservation(AU)