Plant and microbial communities responded to copper and/or tetracyclines in mycorrhizal enhanced vertical flow constructed wetlands microcosms with Canna indica L.

Arbuscular mycorrhizal fungi (AMF) play a significant role in pollutants removal in constructed wetlands (CWs). However, the purification effects of AMF on combined copper (Cu) and tetracycline (TC) pollution in CWs remains unknown. This study investigated the growth, physiological characteristics and AMF colonization of Canna indica L. living in vertical flow CWs (VFCWs) treated for Cu and/or TC pollution, the purification effects of AMF enhanced VFCWs on Cu and TC, and the microbial community structures. The results showed that (1) Cu and TC inhibited plant growth and decreased AMF colonization; (2) the removal rates of TC and Cu by VFCWs were 99.13-99.80% and 93.17-99.64%, respectively; (3) the growth, Cu and TC uptakes of C. indica and Cu removal rates were enhanced by AMF inoculation; (4) TC and Cu stresses reduced and AMF inoculation increased bacterial operational taxonomic units (OTUs) in the VFCWs, Proteobacteria, Bacteroidetes, Firmicutes and Acidobacteria were the dominant bacteria, and AMF inoculation decreased the relative abundance of Novosphingobium and Cupriavidus. Therefore, AMF could enhance the pollutants purification in VFCWs by promoting plant growth and altering the microbial community structures.

The positive effects of inoculation using arbuscular mycorrhizal fungi and/or dark septate endophytes on the purification efficiency of CuO-nanoparticles-polluted wastewater in constructed wetland.

The extent to which, and mechanisms by which, arbuscular mycorrhizal fungi (AMF) and dark septate endophytes (DSE) purify wetlands polluted by metallic nanoparticles (metallic NPs) are not well understood. In this study, micro-vertical flow constructed wetlands (MVFCWs) with the Phragmites australis (reeds)-AMF/DSE symbiont were used to treat CuO nanoparticles (CuO-NPs)-polluted wastewater. The results showed that (1) the removal efficiencies of chemical oxygen demand (COD), total nitrogen (TN), and CuO-NPs in three inoculated groups significantly exceeded those in the control check (CK) groups by 28.94-98.72%, 16.63-47.66%, and 0.53-19.12%, respectively; (2) inoculation with AMF and/or DSE significantly promoted the growth, nutrient content, and photosynthesis of reeds, increased the osmoregulation substance content and antioxidant enzyme activities, and decreased the malondialdehyde and reactive oxygen species contents of reeds under CuO-NPs stress; (3) higher Cu accumulation and smaller transport coefficients were found in the inoculated groups than in the CK group; (4) inoculation with AMF and/or DSE changed the subcellular structure distribution and chemical form of Cu in reeds. We therefore conclude that inoculation with AMF and/or DSE in MVFCWs improves the purification of CuO-NPs-polluted wastewater, and the MVFCW-reeds-AMF/DSE associations exhibit great potential for application in remediation of metallic-NPs-polluted wastewater.

Effects of Aeration on the Formation of Arbuscular Mycorrhiza under a Flooded State and Copper Oxide Nanoparticle Removal in Vertical Flow Constructed Wetlands.

In this study, six vertical flow constructed wetlands (VFCWs) planted with Phragmites australis were operated at different aeration times (4 h day-1 and 8 h day-1), aeration modes (continuous and intermittent), and arbuscular mycorrhizal fungi (AMF) inoculation treatments (inoculation with Rhizophagus intraradices and no inoculation) to explore the effects of different aeration strategies on the formation of arbuscular mycorrhiza under a flooded state in VFCWs. In addition, these VFCWs were further used to treat copper oxide nanoparticle (CuO-NP) wastewater to evaluate the correlations among aeration, colonization, growth, and CuO-NP removal. The highest AMF 28S copy number (1.99×105) and colonization in reed roots, with values of 67%, 21%, and 1% for frequency (F%), intensity (M%), and arbuscule abundance (A%), were observed in the treatment with intermittent aeration for 4 h day-1. Aeration significantly increased the dissolved oxygen (DO) concentration and AMF colonization in VFCWs, thereby promoting plant growth and the purification of the CuO-NPs. However, excessive and continuous aeration had little positive effect on AMF colonization. This study provides a theoretical basis for the application of AMF for improving pollutant removal performance in constructed wetlands.