Unacclimated activated sludge improved nitrate reduction and N2 selectivity in iron filling/biochar systems.

Iron (Fe)-based denitrification is a proven technology for removing nitrate from water, yet challenges such as limited pH preference range and low N2 selectivity (reduction of nitrate to N2) persist. Adding biochar (BC) can improve the pH preference range but not N2 selectivity. This study aimed to improve nitrate reduction and N2 selectivity in iron filling/biochar (Fe/BC) systems with a simplified approach by coupling unacclimated microbes (M) in the system. Factors such as initial pH, Fe/BC ratio, and Fe/BC dosage on nitrate removal efficiency and N2 selectivity were evaluated. Results show that the introduction of microbes significantly enhanced nitrate removal and N2 selectivity, achieving 100 % nitrate removal and 79 % N2 selectivity. The Fe/BC/M system exhibited efficient nitrate reduction at pH of 2-10. Moreover, the Fe/BC/M system demonstrated an improved electrochemical active surface area (ECSA), lower electron transfer resistance and lower corrosion potential, leading to enhanced nitrate reduction. The high i0 value in Fe/BC/M system means more Hads could be generated, thus improving the N2 selectivity. This study provides valuable insights into a novel approach for effective nitrate removal, offering a potential solution to the environmental challenges posed by excessive nitrate in wastewater, surface water and ground water.

Integration of anaerobic digestion and electrodialysis for methane yield promotion and in-situ ammonium recovery.

Ammonia inhibition is a common issue encountered in anaerobic digestion (AD) when treating nitrogen-rich substrates. This study proposed a novel approach, the electrodialysis-integrated AD (ADED) system, for in-situ recovery of ammonium (NH4+) while simultaneously enhancing AD performance. The ADED reactor was operated at two different NH4+-N concentrations (5,000 mg/L and 10,000 mg/L) to evaluate its performance against a conventional AD reactor. The results indicate that the ADED technology effectively reduced the NH4+-N concentration to below 2,000 mg/L, achieving this with a competitive energy consumption. Moreover, the ADED reactor demonstrated a 1.43-fold improvement in methane production when the influent NH4+-N was 5,000 mg/L, and it effectively prevented complete inhibition of methane production at the influent NH4+-N of 10,000 mg/L. The life cycle impact assessment reveals that ADED technology offers a more environmentally friendly alternative by recovering valuable fertilizer from the AD system.

Distinguishing responses of acetoclastic and hydrogenotrophic methanogens to ammonia stress in mesophilic mixed cultures.

A shift from the acetoclastic to the hydrogenotrophic pathway in methanogenesis under ammonia inhibition is a common observation in anaerobic digestion. However, there are still considerable knowledge gaps concerning the differential ammonia tolerance of acetoclastic and hydrogenotrophic methanogens (AMs and HMs), their responses to different ammonia species (NH4+, NH3), and their recoverability after ammonia inhibition. With the successful enrichment of mesophilic AMs and HMs cultures, this study aimed at addressing the above knowledge gaps through batch inhibition/recovery tests and kinetic modeling under varying total ammonia (TAN, 0.2-10 g N/L) and pH (7.0-8.5) conditions. The results showed that the tolerance level of HMs to free ammonia (FAN, IC50=1345 mg N/L) and NH4+ (IC50=6050 mg N/L) was nearly 11 times and 3 times those of AMs (NH3, IC50=123 mg N/L; NH4+, IC50=2133 mg N/L), respectively. Consistent with general belief, the AMs were more impacted by FAN. However, the HMs were more adversely affected by NH4+ when the pH was ≤8.0. A low TAN (1.0-4.0 g N/L) could cause irreversible inhibition of the AMs due to significant cell death, whereas the activity of HMs could be fully or even over recovered from severe ammonia stress (FAN≤ 0.9 g N/L or TAN≤10 g N/L; pH ≤8.0). The different tolerance responses of AMs and HMs might be associated with the cell morphology, multiple energy-converting systems, and Gibbs free energy from substrate-level phosphorylation.

Thermal crosslinking synthesis of ethylene-vinyl acetate copolymer supported floating TiO2 photocatalyst: characterization and photocatalytic performance.

Floating photocatalyst is of extensive interest due to easy recovery and efficient light harvest. Support materials largely determine the stability of floating photocatalysts and their synthesis complexity. Thus, finding proper floating supports is very important. Herein, ethylene-vinyl acetate copolymer (EVA) was investigated as a support to prepare floating TiO2/EVA using a simple thermal crosslinking procedure. Multiple characterization analyses demonstrated that TiO2 was anchored onto EVA surface evenly via hydrogen-bond-enhanced physical crosslinking and remained its virgin crystal structure. Photocatalytic experiments showed that the removal efficiency of Rhodamine B (RhB) by floating TiO2/EVA increased by 33.8% as compared to suspended particle TiO2. The h+ and ·O2- played dominant roles in TiO2/EVA-driven RhB degradation. A 30-day stability test demonstrated that TiO2/EVA had a high thermal, pH, and photo- stability. The three-run reuse test proved that TiO2/EVA exhibited satisfactory reusability. This study provides a new option for floating photocatalyst synthesis.

Photodegradation of roxarsone in the aquatic environment: influencing factors, mechanisms, and artificial neural network modeling.

Roxarsone (ROX), an organoarsenic feed additive, can be discharged into aquatic environment and photodegraded into more toxic inorganic arsenics. However, the photodegradation behavior of ROX in aquatic environment is still unclear. To better understand ROX photodegradation behavior, the influencing factors, photodegradation mechanism, and process modelling of ROX photodegradation were investigated in this study. The results showed that ROX in the aquatic environment was degraded to inorganic As(III) and As(V) under light irradiation. The degradation efficiency was enhanced by 25% with the increase of light intensity from 300 to 800 µW/cm2 via indirect photolysis. The photodegradation was temperature dependence, but was only slightly affected by pH. Nitrate ion (NO3-) had an obvious influence, but sulfate, carbonate, and chlorate ions had a negligible effect on ROX degradation. Dissolved organic matter (DOM) in the solution inhibited the photodegradation. ROX photodegradation was mainly mediated by reactive oxygen species (in the form of single oxygen 1O2) generated through ROX self-sensitization under irradiation. Based on the data of factors affecting ROX photodegradation, ROX photodegradation model was built and trained by an artificial neural network (ANN), and the predicted degradation rate was in good agreement with the real values with a root mean square error of 1.008. This study improved the understanding of ROX photodegradation behavior and provided a basis for controlling the pollution from ROX photodegradation.

Stimulatory effects of biochar addition on dry anaerobic co-digestion of pig manure and food waste under mesophilic conditions.

The stimulatory effect of biochar addition on dry anaerobic digestion (AD) has been rarely investigated. In this study, the effects of commonly used biochars (bamboo, rice husk, and pecan shell) on dry co-AD were investigated using mesophilic batch digesters fed with pig manure and food waste as substrates. The results show that the specific methane yield was mildly elevated with the addition of biochars by 7.9%, 9.4%, and 12.0% for bamboo, rice husk, and pecan shell-derived biochar additions, respectively. Biochar did facilitate the degradation of poorly biodegradable organics. In comparison, there was no significant effect on the peak methane production rate by the supplementation of the selected biochars. Among the three mechanisms of enhancing methanogenesis by biochar (buffering, providing supporting surface, and enhancing electron transfer), the first two mechanisms did not function significantly in dry co-AD, while the third mechanism (i.e., enhancing electron transfer) might play an important part in dry AD process. It is recommended that the utilization of biochar for the enhancement of biomethanation in dry AD should be more focused on mono digestion in future studies.