Blue Photons from Broad-Spectrum LEDs Control Growth, Morphology, and Coloration of Indoor Hydroponic Red-Leaf Lettuce.

For indoor crop production, blue + red light-emitting diodes (LEDs) have high photosynthetic efficacy but create pink or purple hues unsuitable for workers to inspect crops. Adding green light to blue + red light forms a broad spectrum (white light), which is created by: phosphor-converted blue LEDs that cast photons with longer wavelengths, or a combination of blue, green, and red LEDs. A broad spectrum typically has a lower energy efficiency than dichromatic blue + red light but increases color rendering and creates a visually pleasing work environment. Lettuce growth depends on the interactions of blue and green light, but it is not clear how phosphor-converted broad spectra, with or without supplemental blue and red light, influence crop growth and quality. We grew red-leaf lettuce 'Rouxai' in an indoor deep-flow hydroponic system at 22 °C air temperature and ambient CO2. Upon germination, plants received six LED treatments delivering different blue fractions (from 7% to 35%) but the same total photon flux density (400 to 799 nm) of 180 µmol·m-2·s-1 under a 20 h photoperiod. The six LED treatments were: (1) warm white (WW180); (2) mint white (MW180); (3) MW100 + blue10 + red70; (4) blue20 + green60 + red100; (5) MW100 + blue50 + red30; and (6) blue60 + green60 + red60. Subscripts denote photon flux densities in µmol·m-2·s-1. Treatments 3 and 4 had similar blue, green, and red photon flux densities, as did treatments 5 and 6. At the harvest of mature plants, lettuce biomass, morphology, and color were similar under WW180 and MW180, which had different green and red fractions but similar blue fractions. As the blue fraction in broad spectra increased, shoot fresh mass, shoot dry mass, leaf number, leaf size, and plant diameter generally decreased and red leaf coloration intensified. Compared to blue + green + red LEDs, white LEDs supplemented with blue + red LEDs had similar effects on lettuce when they delivered similar blue, green, and red photon flux densities. We conclude that the blue photon flux density in broad spectra predominantly controls lettuce biomass, morphology, and coloration.

Bispecific antibody targeting TROP2xCD3 suppresses tumor growth of triple negative breast cancer.

BACKGROUND: Triple negative breast cancer (TNBC) is a subtype of breast cancers with poor prognosis and targeted drug therapies are limited. To develop novel and efficacious therapies for TNBC, we developed a bispecific antibody F7AK3 that recognizes both trophoblast cell surface antigen 2 (TROP2) and CD3 and evaluated its antitumor activities both in vitro and in vivo. METHODS: The binding affinities of F7AK3 to the two targets, TROP2 and CD3, were evaluated by surface plasmon resonance. Binding of F7AK3 to TNBC cells and T cells were evaluated by flow cytometry. Immunofluorescent staining was performed to demonstrate the interactions between T cells with TNBC cells. The cytotoxicity of T cells against TNBC cell lines and primary tumor cells mediated by F7AK3 were determined in vitro. In vivo antitumor activity of F7AK3 was investigated in a xenograft TNBC tumor model, using immunodeficient mice that were reconstituted with human peripheral blood mononuclear cells. RESULTS: We demonstrated that F7AK3 binds specifically to human TROP2 and CD3 antigens, as well as TNBC cell lines and primary tumor cells. Human T cells can only be activated by F7AK3 in the presence of target tumor cells. F7AK3 recruits T cells to TROP2+ tumor cells in vitro and into tumor tissues in vivo. Antitumor growth activity of F7AK3 is observed in a xenograft TNBC tumor model. CONCLUSION: This study showed the antitumor potential of an anti-TROP2xCD3 bispecific antibody F7AK3 to TNBC tumor cells both in vitro and in vivo. These data demonstrate that F7AK3 has the potential to treat TNBC patients, which warrants further preclinical and clinical evaluation of the F7AK3 in advanced or metastatic TNBC patients.

Growth Responses of Red-Leaf Lettuce to Temporal Spectral Changes.

Lighting is typically static for indoor production of leafy greens. However, temporal spectrum differentiation for distinct growth phases can potentially control age-specific desirable traits. Spectral effects can be persistent yet dynamic as plants mature, necessitating characterization of time-dependent responses. We grew red-leaf lettuce (Lactuca sativa L.) "Rouxai" in a growth room at 23°C and under a 20-h photoperiod created by warm-white (WW), blue (B; peak = 449 nm), green (G; peak = 526 nm), red (R; peak = 664 nm), and/or far-red (FR; peak = 733 nm) light-emitting diodes. From day 0 to 11, plants received six static lighting treatments with the same total photon flux density (400-800 nm): WW180, R180, B20R160, B20G60R100, B20R100FR60, or B180 (subscripts denote photon flux densities in µmol⋅m-2⋅s-1). On day 11, plants grown under each of the six treatments were transferred to all treatments, which created 36 temporal spectrum alternations. Plant growth, morphology, and coloration were measured on days 11 and 25. Increasing B radiation from 0 to 100% in static treatments decreased shoot fresh and dry weights and increased foliage redness of seedlings and mature plants. Compared to B20R160, B20R100FR60 increased shoot fresh weight, but not dry weight, on both days. However, other phenotypic responses under static treatments changed over time. For example, leaf length under B180 was 35% lower on day 11 but similar on day 25 compared to that under R180. In the B20 background, substituting G60 for R radiation did not influence shoot weight on day 11 but decreased it by 19% on day 25. When plants were switched from one treatment to another on day 11, the treatments applied before day 11 influenced final shoot weight and, to a lesser extent, leaf length and foliage coloration on day 25. In comparison, effects of the treatments applied after day 11 were more pronounced. We conclude some phenotypic responses to light quality depend on time and sequential light quality treatments had cumulative effects on lettuce growth. The temporal complexity of spectral responses is critical in photobiological research and creates opportunities for time-specific spectrum delivery to optimize crop characteristics.

An alternative for the anode materials of nickel metal hydride batteries: an AB3-type La0.6Gd0.2Mg0.2Ni2.6Co0.3Al0.1 hydrogen storage alloy.

In order to satisfy the demand for the cyclic stability of commercial Ni-MH anodes, a PuNi3-type La0.6Gd0.2Mg0.2Ni2.6Co0.3Al0.1 alloy with excellent overall electrochemical properties was prepared by annealing the as-cast alloy sample at different temperatures for a week. The alloy had the highest PuNi3-type content of 86.9 wt% (1073 K), which offered a capacity retention of 69.6% after 100 cycles. However, 23.7 wt% PuNi3 type phase of the alloy constantly converted into the Ce2Ni7 type phase within a temperature increase of 50 °C, which improved the capacity retention by 12.1% under the same discharge capacity. We found that the addition of Gd did not change the stacked [LaMgNi4]/[LaNi5] superlattice and it maintained the structural stability of the crystal as well as its anti-corrosion, which is also a key factor to improve cyclic stability. These findings imply that alloys with both PuNi3-type and Ce2Ni7-type multiphase structures can be considered as a new choice for hydrogen storage.

Regulation of flowering by green light depends on its photon flux density and involves cryptochromes.

Photoperiodic lighting can promote flowering of long-day plants (LDPs) and inhibit flowering of short-day plants (SDPs). Red (R) and far-red (FR) light regulate flowering through phytochromes, whereas blue light does so primarily through cryptochromes. In contrast, the role of green light in photoperiodic regulation of flowering has been inconsistent in previous studies. We grew four LDP species (two petunia cultivars, ageratum, snapdragon and Arabidopsis) and two SDP species (three chrysanthemum cultivars and marigold) in a greenhouse under truncated 9-h short days with or without 7-h day-extension lighting from green light (peak = 521 nm) at 0, 2, 13 or 25 µmol m-2  s-1 or R + white (W) + FR light at 2 µmol m-2  s-1 . Increasing the green photon flux density from 0 to 25 µmol m-2  s-1 accelerated flowering of all LDPs and delayed flowering of all SDPs. Petunia flowered similarly fast under R + W + FR light and moderate green light but was shorter and developed more branches under green light. To be as effective as R + W + FR light, saturation green photon flux densities were 2 µmol m-2  s-1 for LDP ageratum and SDP marigold and 13 µmol m-2  s-1 for LDP petunia. Snapdragon was the least sensitive to green light. In Arabidopsis, cryptochrome 2 mediated promotion of flowering under moderate green light, whereas both phytochrome B and cryptochrome 2 mediated that under R + W + FR light. We conclude that 7-h day-extension lighting from green light-emitting diodes can control flowering of photoperiodic ornamentals and that in Arabidopsis, cryptochrome 2 mediates promotion of flowering under green light.