Overexpression of ETV2 in BMSCs promoted wound healing in cutaneous wound mice by triggering the differentiation of BMSCs into endothelial cells and modulating the transformation of M1 phenotype macrophages to M2 phenotype macrophages.

This study aimed to investigate the effects of E26-transformation-specific variant-2 (ETV2) overexpression on wound healing in a cutaneous wound (CW) model and clarify associated mechanisms. pLVX-ETV2 lentivirus expressing ETV2 was constructed and infected into BMSCs to generate ETV2-overexpressed BMSCs (BMSCs+pLVX+ETV2). The RT-PCR assay was applied to amplify ETV2, VE-cadherin, vWF, ARG-1, IL-6, iNOS, TGF-ß, IL-10, TNF-α. Western blot was used to determine expression of VE-cadherin and vWF. ETV2 induced differentiation of BMSCs into ECs by increasing CDH5/CD31, triggering tube-like structures, inducing Dil-Ac-LDL positive BMSCs. ETV2 overexpression increased the gene transcription and expression of VE-cadherin and vWF (P<0.01). Transcription of M1 phenotype specific iNOS gene was lower and transcription of M2 phenotype specific ARG-1 gene was higher in the RAW264.7+BMSCs+ETV2 group compared to the RAW264.7+BMSCs+pLVX group (P<0.01). ETV2 overexpression (RAW264.7+BMSCs+ETV2) downregulated IL-6 and TNF-α, and upregulated IL-10 and TGF-ß gene transcription compared to RAW264.7+BMSCs+pLVX group (P<0.01). ETV2-overexpressed BMSCs promoted wound healing in CW mice and triggered the migration of BMSCs to the wound region and macrophage activation. ETV2-overexpressed BMSCs promoted collagen fibers and blood vessel formation in the wound region of CW mice. In conclusion, this study revealed a novel biofunction of ETV2 molecule in the wound healing process. ETV2 overexpression in BMSCs promoted wound healing in CW mice by triggering BMSCs differentiation into endothelial cells and modulating the transformation of M1 pro-inflammatory and M2 anti-inflammatory macrophages in vitro and in vivo.

On-orbit space optical communication demonstration with a 22†s acquisition time.

The inherent small divergence angle of space optical communication poses significant challenges to establishing communication links. Therefore, laser link acquisition becomes a crucial technology in optical communication, with a primary focus on reducing the acquisition time effectively. Conventional optical communication acquisition techniques fail to address the error effects caused by satellite insertion into the Earth orbit, thus unable to meet the rapid establishment demands of laser links in satellite optical communication networks. In this regard, we propose a novel, to the best of our knowledge, on-orbit calibration method for laser communication terminals to our knowledge. Leveraging stellar observations, this method achieves efficient on-orbit calibration with only a single observation. Theoretical analysis and on-orbit experimental results demonstrate that this approach effectively enhances an open-loop pointing accuracy, offering promising prospects for achieving a rapid establishment of optical communication links in ultra-long-distance optical communication missions.

Suppurative tenosynovitis with chronic carpal tunnel syndrome due to corticosteroid injections: A case report.

INTRODUCTION: Steroid hormone blocking is a common treatment for tenosynovitis. However, local steroid hormone blocking requires careful attention to the local inflammatory response, as infection can cause severe local soft tissue inflammation and damage. PRESENTATION OF CASE: A 46-year-old female patient received local steroid hormone blocking treatment for tenosynovitis of the right thumb at another hospital 3 months earlier. Five days later, the patient gradually experienced redness, swelling, and pain in her right hand. By day 7, the symptoms worsened with increased swelling, wrist pain, and finger numbness. After the carpal tunnel incision, the patient's symptoms improved upon admission to the local hospital's emergency department. However, 2 weeks post-operation, she experienced recurring numbness, pain, discomfort, and local sinus exudation in her right hand, which worsened over 2 months, prompting the patient to seek outpatient treatment at our hospital. DISCUSSION: An increase in local content within the wrist joint content is a major cause of carpal tunnel syndrome. Repeated stimulation of the inflamed tissues often leads to the development of granulomatous hyperplasia. Hyperplastic granulomas often produce local compression. If located in the peripheral nerve duct, it may cause nerve entrapment and lead to peripheral nerve injury. Surgery is often required to excise the hyperplastic tissue and release the entrapped nerve. CONCLUSION: Prompt and thorough debridement is necessary for addressing local soft tissue infections caused by suppurative tenosynovitis. Failure to do so may result in recurrent local granuloma hyperplasia and the development of local compression diseases, especially in wrist median nerve compression cases.

On-orbit demonstration of inter-satellite free-space optical stable communication enabled by integrated optical amplification of HPA and LNA.

Satellite free-space optical (FSO) communication is very promising in improving the bandwidth and capacity of space information networks in the future. However, the inter-satellite transmission distance of over 1000 km leads to unstable optical beam pointing, acquisition, and tracking and then generates optical power jitter by a large margin before detection-demodulation. Therefore, it is difficult to realize high-stability and long-time FSO communication between satellites due to the generated bit error rate (BER) by jitter. In this paper, we report an autonomously self-designed and high-integration laser communication payload (LCP) and on-orbit-demonstrated inter-satellite 145 min, zero-BER FSO stable communication with a line rate of 2.8 Gbps. Moreover, based on the inter-satellite laser communication link, a video phone was clearly implemented for more than 10 min, and authentic user data transmitted 459,149 packets, achieving results of zero-packet loss. Summarily, this on-orbit experiment demonstrated an excellent performance of the LCP owing to the distinctive design of integrating a high-power amplifier and low-noise amplifier optical amplification function. Our space mission was successfully completed, and the on-orbit demonstration results may offer a significant reference for the field of satellite laser communication and space information networks.

Beam Scanning and Capture of Micro Laser Communication Terminal Based on MEMS Micromirrors.

With the development of space laser communication and the planned deployment of satellite Internet constellations, there is a growing demand for microminiature laser communication terminals. To meet the requirements of size, weight and power (SWaP), miniaturized terminals require smaller drive components to complete on-orbit scanning and capture, which must be fast and efficient to enable satellite laser communication networks. These miniaturized laser communication terminals are highly susceptible to the impact of the initial pointing accuracy of the laser beam and microvibrations of the satellite platform. Therefore, this paper proposes a laser scanning-capture model based on a Micro-electromechanical Systems (MEMS) micromirror that can provide a fast, large-scale scanning analysis. A scanning overlap factor is introduced to improve the capture probability under the influence of microvibrations. Finally, experimental analysis was carried out to verify the effectiveness of the proposed model, which can establish a theoretical basis for future ultra-long-distance microspace laser communication.

Predictive value of bone morphogenetic protein-7, thromboxane A2, and osteoprotegerin for prognosis of patients with distal radius fractures.

OBJECTIVES: This study aims to investigate the predictive value of bone morphogenetic protein-7 (BMP-7), thromboxane A2 (TXA2), and osteoprotegerin (OPG) for the prognosis of patients with distal radius fractures. PATIENTS AND METHODS: Between January 2021 and January 2022, a total of 124 patients (71 males, 53 females; mean age: 49.8±5.1 years; range, 34 to 68 years) with distal radius fractures were included in the fracture group. Healthy volunteers receiving physical examination in our hospital in the same period were included in the control group (n=50; 29 males, 21 females; mean age: 50.1±5.4 years; range, 35 to 68 years). The expressions of BMP-7, TXA2, and OPG in the peripheral blood were detected. In the fracture group, 124 patients underwent internal fixation after inclusion and followed for six months. The prognosis was evaluated based on the Gartland & Werley scoring system for wrist joint function. The factors influencing prognosis were analyzed, and the predictive values of BMP-7, TXA2, and OPG were calculated. RESULTS: Age, fracture classification, early loss of palmar tilt, late loss of palmar tilt, time to return to exercise after surgery, BMP-7, TXA2, and OPG were all factors influencing the prognosis (p<0.05). For predicting the prognosis, the area under the ROC curve of BMP-7 + TXA2 + OPG (0.928) was significantly larger than those of BMP-7 (0.810), TXA2 (0.856) and OPG (0.823) alone, and BMP-7 + TXA2 + OPG had the highest predictive efficiency. The BMP-7 was negatively correlated with TXA2 (r=-0.471), but positively correlated with OPG (r=0.437). CONCLUSION: The combined detection of BMP-7, OPG, and TXA2 is highly valuable for predicting the prognosis of patients with distal radius fractures.

SOX9 Inhibits the Progression of Osteonecrosis of the Femoral Head via the Activation of the Wnt/Beta-Catenin Pathway.

Background: In this study, we aimed to explore whether the SRY-box transcription factor 9 (SOX9) can play protective roles against the occurrence and development of osteonecrosis of the femoral head (ONFH) by regulating the proliferation, apoptosis, and osteogenic differentiation of human bone marrow stromal cells (hBMSCs) via the Wnt/ß-catenin pathway.Methods: We used 1600 mg of glucocorticoid (GC) to induce hBMSCs to establish an ONFH cell model and performed various experiments. Reverse transcription-quantitative polymerase chain reaction and western blotting assays were used to determine the expression levels of SOX9 and osteoblast markers, such as the RUNX family transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osterix, Wnt3a, and ß-catenin. An ALP detection kit was used to measure the ALP activity. Flow cytometry and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays were performed to determine the cell viability.Results: GC treatment decreased the expression levels of RUNX2, ALP, and osterix, suppressed ALP activity, and inhibited SOX9 expression. SOX9 overexpression promoted GC-induced cell proliferation and decreased cell apoptosis. Additionally, hBMSCs were transfected with SOX9-small interfering RNA in GC treatment, and SOX9 knockdown was found to suppress the osteogenic differentiation of cells and decrease their viability.Conclusion: Our results revealed that SOX9 is related to the Wnt/ß-catenin pathway in ONFH. Moreover, SOX9 participated in ONFH development by activating the Wnt/ß-catenin pathway.

A novel modified Winograd surgical tricks and tips with a "bird flap" for the treatment of ingrown toenails.

BACKGROUND: The Winograd technique is the most commonly used surgical treatment for ingrown toenails. We describe a novel modified approach, more effective and simpler to perform with a better cosmetic outcome. METHODS: We retrospectively included 45 and 39 patients with 67 and 58 ingrown toenails who underwent our modified Winograd technique and the Winograd technique, respectively, from July 2017 to June 2020, and obtained data after 3, 6, and 12 months of follow-up. RESULTS: No significant differences in the postoperative time taken to return to regular activities in the modified Winograd and traditional Winograd groups (p = 0.103) and regarding the recurrence in both groups (p = 0.055) were found. The extent of proximal germinal matrix exposure with the modified Winograd technique was significantly more clearly revealed than in the traditional Winograd method contextually (p < 0.05). The postoperative appearance satisfaction rate was significantly higher in the modified Winograd group than in the traditional Winograd group (p = 0.029). CONCLUSION: The modified Winograd technique is effective in treating ingrown toenails.

Artificial Neural Network Model for the Prediction of Thermal Conductivity of Saturated Liquid Refrigerants and n-Alkanes.

In this paper, a feed-forward back-propagation artificial neural network (ANN) is proposed to correlate and predict the thermal conductivity from the triple point temperature up to 0.98 times critical temperature (T c) for 23 refrigerants and 11 n-alkanes. It requires the temperature (T) as well as the molecular mass (M), acentric factor (ω), critical temperature, and critical pressure (P c) as input variables. The optimal ANN model is obtained by a trial-and-error procedure and consists of the input layer and the output layer together with one hidden layer with seven neurons. This ANN model can not only correlate the thermal conductivity but also accurately predict the thermal conductivity of refrigerants and n-alkanes. The correlation coefficients (R) in the training and testing phases are 0.9994 and 0.9993, respectively. Furthermore, the average absolute deviation (AAD) values are less than 1% for 14 out of 34 fluids, less than 2% for 28 fluids, and less than 4.5% for all the considered fluids.

Exhausting circ_0136474 and Restoring miR-766-3p Attenuate Chondrocyte Oxidative Injury in IL-1ß-Induced Osteoarthritis Progression Through Regulating DNMT3A.

Circular RNA circ_0136474 is a new contributor of human osteoarthritis (OA) by suppressing chondrocyte proliferation. However, its role and mechanism in OA chondrocyte injury remain ill defined. Herein, we performed real-time quantitative PCR to detect RNA expression of circ_0136474, microRNA (miR)-766-3p, and DNA methyltransferase 3A (DNMT3A) and utilized Western blotting to measure protein expression of DNMT3A, matrix metalloproteinase-1 (MMP1), MMP13, collagen II, proliferating cell nuclear antigen (PCNA) and B cell lymphoma (Bcl)-2, and Bcl-2-associated X protein (Bax). Direct interaction between miR-766-3p and circ_0136474 or DNMT3A was confirmed by bioinformatics algorithms, dual-luciferase reporter assay, and RNA immunoprecipitation. Functional experiments including cell counting kit-8 assay, flow cytometry, and special assay kits were employed to measure oxidative injury in interleukin (IL)-1ß-induced OA-like chondrocytes. First, IL-1ß administration induced cell viability inhibition, collagen II suppression, and promotion of MMP1 and MMP13 in human chondrocyte CHON-001 cells. Expression of circ_0136474 and DNMT3A was upregulated, and miR-766-3p was downregulated in human OA cartilages and IL-1ß-induced CHON-001 cells. Functionally, both blocking circ_0136474 and upregulating miR-766-3p could rescue cell viability and levels of PCNA, Bcl-2, reduced glutathione (GSH), and total superoxide dismutase (SOD), and attenuate apoptosis rate and levels of Bax, reactive oxygen species (ROS), and lipid peroxidation malondialdehyde (MDA). Mechanically, circ_0136474 served as miR-766-3p sponge to govern miR-766-3p-targeted DNMT3A expression. Accidently, restoring DNMT3A counteracted the miR-766-3p upregulation role, and silencing miR-766-3p weakened circ_0136474 knockdown effect in IL-1ß-induced CHON-001 cells. In conclusion, exhausting circ_0136474 could mitigate OA chondrocyte oxidative injury through regulating miR-766-3p/DNMT3A axis.

An experimental investigation of the dynamic fracture behavior of 3D printed nacre-like composites.

The dynamic fracture behavior of 3D printed nacre-like composites was fully characterized by performing three-point bending dynamic fracture experiments. Nacre-like specimens with brick and mortar microstructures were fabricated with dual-material 3D printing technology. A brittle rigid material and a rubber-like material were selected for the brick layers and adhesive of the composite, respectively. First, two crack propagation paths of specimens under impact loading were observed. The dynamic behavior of the specimen under three-point bending impact loading showed a sensitivity to the impact velocity. Then, the influences of the brick aspect ratio and adhesive thickness on the fracture failure were determined. The results showed that for the case of brick aspect ratio of 1, the peak force and peak effective surface energy increase with increasing adhesive thickness, meaning that the specimen with a larger adhesive thickness had a greater bearing capacity. For the case of brick aspect ratio of 3, when the adhesive thickness was small, the nacre-like composites exhibited a lower bearing capacity than the rigid bulk material under impact loading. However, as the adhesive thickness increased, the bearing capacity and fracture toughness of the composite improved and ultimately exceeded those of the rigid bulk material. For the specimens with a small brick aspect ratio, the cracks propagated primarily along the soft adhesive, whereas for the specimens with a large brick aspect ratio, the cracks were more likely to propagate through the hard brick. Additionally, a plateau appeared in the load-displacement curves of the specimens with a large brick aspect ratio.