Complete mitochondrial characteristics and phylogenetic analysis of Deilephila elpenor (Linnaeus, 1758) (Lepidoptera: Sphingidae).

Deilephila elpenor is widely distributed in countries of Asia and Central Europe, and the larva is recognized as significant agriculture pest. The complete mitochondrial genome of D. elpenor is 15,372 bp in length. It contains 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a control region. TAA is utilized as the termination codon for most PCGs, however, cox1 and cox2 use the incomplete termination codon T, and nad3 uses TAG as the termination codon. UUA (Leu) is the most frequently used codon, GCG (Ala) and CCG (Pro) are the least frequently used codons. In addition, we selected 15 species sequences closest to this species from NCBI, and used Manduca quinquemaculata and Manduca sexta (Lepidoptera: Smerinthinae) as the outgroup. Phylogenetic analysis suggested that D. elpenor was the most closely related to genus Theretra, genus Rhagastis and Cechenena minor.

Characterization of the complete mitochondrial genome sequence of Smerinthus caecus (Lepidoptera: Sphingidae).

In this study, we sequenced and analyzed the complete mitogenome of Smerinthus caecus Ménétriés, 1857. The mitogenome of S. caecus is a circular structure, and 15,363 bp long in size and encodes 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and a control region (CR). An extremely high AT bias of 79.2% was found in the nucleotide composition of mitogenome. Most of the PCGs used ATN as the start codon and TAA or TAG as the stop codon, which is similar to most other insect mitogenomes, except cox1, which starts with CGA. The phylogeny of Smerinthinae was reconstructed using a maximum-likelihood method, a total of 33 mitogenomes were sampled for phylogenetic analyses. The subfamily Langiinae was selected as outgroup. The results confirmed the position of S. caecus in the Smerinthinae, in which Smerinthus caecus was placed as the sister taxon to Smerinthus planus, then to Laothoe amurensis.

The first complete mitochondrial genome of the hawkmoth Marumba saishiuana (Lepidoptera: Sphingidae) and insights into its phylogenetic position.

In this study, we sequenced and analyzed the complete mitogenome of Marumba saishiuana Okamoto, 1924. The complete mitogenome sequence of M. saishiuana is circular, 15,662 bp in size and encodes 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and a control region (CR). Nucleotide composition is highly biased toward A + T nucleotides (81.2%). Most of 13 PCGs initiate with the standard start codon of ATN, except cox1, which starts with CGA. Phylogenetic analyses were performed using nucleotide sequences. A total of 32 Smerinthinae species were selected. The topology based on mitogenome showed that M. saishiuana, M. gaschkewitschii, and M. sperchius formed a clade, and this indicated that M. saishiuana was a member of genus Marumba. Polyptychus trilineatus was the most closely related to genus Marumba on the phylogenetic tree reconstructed by mitogenomes.

Next-generation sequencing yields the complete mitochondrial genome of Rhodoprasina callantha (Lepidoptera: Sphingidae) and its evolutionary status.

In this study, we sequenced and analyzed the complete mitochondrial genome of Rhodoprasina callantha Jordan, 1929. The complete mitochondrial genome sequence of R. callantha was 15,343 bp in size and encoded 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNA), 22 transfer RNA genes (tRNA) and one control region (CR). The nucleotide composition of mitogenome was highly biased to A and T. Most protein-coding genes (PCGs) initiate with the standard start codon of ATN and terminate with the typical stop codon TAA/TAG. The phylogeny of Sphingidae based on nucleotide sequences of PCGs recovered the monophyly of subfamilies of Sphingidae with high support values. Langinae was the first subfamily diverged in Sphingidae, which was in accordance with previous study. R. callantha was the member of subfamily Smerinthinae, and closely related to the genus Marumba.

Phylogenetic relationship and characterization of the complete mitochondrial genome sequence of Smerinthus planus (Lepidoptera: Sphingidae).

In this study, the complete mitochondrial genome of Smerinthus planus Walker, 1856 was sequenced and analyzed. This mitochondrial genome is circular, 15,375 bp long, and includes 37 typical metazoan mitochondrial genes (13 protein-coding genes (PCGs), two ribosomal RNA genes, and 22 transfer RNA genes) and an A + T-rich region. Nucleotide composition is highly biased toward A + T nucleotides (80.1%). All 13 PCGs initiate with the standard start codon of ATN and terminate with the typical stop codon TAA/TAG. Phylogenetic analyses were performed using amino acids of 13 PCGs which shows that S. planus is closely related to Barbourion lemaii.

The complete mitochondrial genome sequence of the hawkmoth, Ambulyx tobii (Lepidoptera: Sphingidae) and phylogenetic analysis.

In this study, we sequenced and analyzed the complete mitochondrial genome of Ambulyx tobii to compare mitochondrial genome structures and reconstruct phylogenetic relationships. The complete mitochondrial genome sequence of A. tobii is circular, 15,343 bp in size and encodes 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and a control region (CR). Nucleotide composition is highly biased toward A + T nucleotides (81.2%). Most PCGs initiate with the standard start codon of ATN and terminate with the typical stop codon TAA/TAG. Phylogenetic analyses were performed using both 13 PCGs and whole mitochondrial genomes showed that A. tobii is closely related to A. substrigilis.