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Objective To investigate the efficacy and safety of short-term intensive insulin aspart injection (Novo-rapid) therapy hepatogenic diabetes with liver decompensation patients. Mothods A two weeks randomized,open-la-bel, confrolled trial comparing Novolin 30R with short-term intensive Novorapid was performed. 100 cases were ran-domly divided into treatment group and control group,50 cases for each. The two groups were given traditional medical treatment, while the control group received Novolin 30R twice per day,the treatment group received Novorapid thrice per-day. The indicators such as FBG,2hPG,HbA1c, Ins,C-P,hypoglycemia incidence and liver function (ALT, AST, ALB and TBIL)were evaluated,β-cell function(HOMA-β)and insulin resistance index(HOMA-IR) were calculated. The changes of above indicators before and after therapy were compared between two groups. Results After the treat-ment,the FBG,2hPG,HbA1c,HOMA-IR and liver function of two groups were respectively lower than those before(P<0.05),hypoglycemia incidence of the treatment group were lower than those of the control group(P<0.05),while the differences between two groups was significant(P<0.05). Conclusion Short-term intensive Novorapid therapy for hep-atogenic diabetes with liver decompensation can reduce FBG and 2hPG,reduce the incidence of hypoglycemia, im-prove liver function,and also reduce insulin resistance. Meanwhile,the therapy is a more effective and safer therapy.
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Objective To investigate the relationship between HBV antigen variationandthe cause of deathin the HBV-related acute-on-chronic liver failure (ACLF). Methods Total of 127 HBV-ACLF patients were divided into survival group and death group, the cause of death, the constitution ratio of mutant rate pre-C region, C gene region and BCP region of two groups were analyzed than before, then MELD and Child-Pugh score system were compared through mul-tivariate Logistic regression analysis to discuss the risk degree of different mutants. Results In 127 cases of HBV-A-CLF patients, 329 mutants were detected between the survival group (n=76) and the death group (n=51). The differ-ences of A1762T/G1764A, G1896A and L60V were significant in the death group than in the survival group according to the results of multivariate Logistic regression analysis (P<0.05), and the scores of MELD were significant difference than the scores of Child-Pugh in the two groups(P<0.05). Conclusion The mutants of A1762T/G1764A, G1896A and L60V in HBV-ACLF are independent risk factors lead to its death, MELD score over matches Child-Pugh score,and MELD score for judging the prognosis and outcome of HBV-ACLF has important significance.
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Fumonisin B1 (FB1) is a carcinogenic mycotoxin found in commodities such as corn and corn-originated products. An aptamer-based method for detection of FB1 was developed using the fluorescent dye PicoGreen, which can recognize and bind double-stranded DNA. A peak fluorescence of PicoGreen was obtained in 15 min in the presence of FB1 aptamer, which formed a double-stranded hybridizer DNA with its complementary strand. The excitation and emission wavelengths for PicoGreen detection were 480 nm and 520 nm, respectively. The sensitivity of this aptamer/PicoGreen-based method was 0.1 μg/L. This method showed a good linearity for FB1 concentration ranging from 0.1 to 1 μg/L. The entire detection procedure for FB1 could be completed within 40 min. No cross reactions were observed with any other mycotoxins against aflatoxin B1, ochratoxin A, citrinin and zearalenone, demonstrating high specificity towards FB1 aptamer. Agreement between commercial, antibody-based enzyme-linked immunosorbent assay (ELISA) kit and aptamer method was excellent with a kappa value of 0.857. Taken together, this aptamer/PicoGreen-based method is more cost-effective, time-saving and useful than ELISA for detection of FB1.
