RESUMO
The yield of winter wheat in Shandong Province is of great significance for ensuring regional and national food security. To reduce the risk of production loss, the WOFOST model was used to simulate the winter wheat growth to obtain the quantitative and dynamic information. Based on the observational data, a moisture control experiment and the trial and error method, the applicability and drought simulation of the WOFOST model were evaluated for winter wheat growth. For the simulation of the seedling period, flowering period, and maturity period of winter wheat in Shandong Province, the R2 were 0.95, 0.69, and 0.68 respectively. The D-index were 0.99, 0.89, and 0.86 respectively. The mean absolute error (mAE) were 1.3, 4.3, and 4.1 respectively. And the nRMSE were 0.65%, 4.3%, and 3.2%, respectively. For the yield simulation, the R2, D-index, mean relative error (mRE), and nRMSE were 0.48, 0.82, 10.5% and 12.8%, respectively. For the yield simulation under drought stress, the R2, D-index, mRE, and nRMSE were 0.77, 0.93, 7.1%, and 7.4%, respectively. An evaluation index system was built through four different degrees of drought treatment between the jointing period and the flowering period. With the aggravation of drought, the growth indicators about the total above ground production (TAGP), maximum leaf area index (MAXLAI), total dry weight of leaves (TWLV), and total dry weight of stems (TWST) decreasing by 13.6-41.0%, 37.8-56.5%, 19.4-42.1%, and 20.3-51.2%, respectively. The results showed that this model could adequately simulate the formation process of yield under both normal and drought conditions.
RESUMO
OBJECTIVE：To study the prot ective effects of crocin （CR）against triptolide （TP）-induced visceral organ injury in mice，and to provide reference for the studying TP compatibility and detoxification. METHODS ：Fifty mice were randomly divided into normal group ，TP low-dose and high-dose groups （i.e. TP-L group ，TP-H group ，with 300，600 μg/kg），TP low-dose and high dose combined with CR groups （i.e. TP-L+CR group ，TP-H+CR group ，with 300 μg/kg TP+100 mg/kg CR ，600 μg/kg TP+ 100 mg/kg CR ），with 10 mice in each group. Except for normal group ，other groups were given relevant medicine intragastrically ， once a day ，for consecutive 7 d. The body weight of mice was weighted every day ，and their death was recorded. After last administration，the mice were sacrificed ，and the heart ，liver，kidney and testis were taken ，and the organ index was calculated ； serum levels of ALT ，AST，BUN and Scr ，the activity of T-SOD and the contents of MDA were all determined. The pathological changes of heart ，liver，kidney and testis were observed ；mRNA expression of Bcl- 2，Bax and caspase- 3 in liver tissue were determined. RESULTS ：Three，five，two and three mice in TP-L group ，TP-H group ，TP-L+CR group and TP-H+CR group died respectively，and the survival rates were 70％，50％，80％ and 70％，respectively. Compared with normal group ，the body weight （7th day of experiment ），heart index ，liver index ，kidney index （except for TP-L group ），testicular index ，T-SOD activity and mRNA expression of Bcl- 2 in liver tissue ，serum levels of ALT （except for TP-L group ），AST（except for TP-L group ），BUN and Scr，MDA content and mRNA expression of Bax ，mRNA expression of caspase- 3 in liver tissue were increased significantly （P＜ 0.05 or P＜0.01）. There were obvious pathological changes in heart ，liver，kidney and testis tissue. Compared with the same dose of TP alone group ，the above indexes of TP combined with CR group were improved in varying degrees. Except for the renal index and serum ALT level of TP-L+CR group ，there was statistical significance for all indexes （P＜0.05 or P＜0.01）；the pathological injuries of heart ，liver，kidney and testis were significantly improved. CONCLUSIONS ：CR can relieve the damage of heart ， liver，kidney and testis induced by TP ，which may be related to the antioxidant stress of CR.
